Trait stacking in transgenic crops: Challenges and opportunities
Abstract
In recent years, there has been a rapid increase in the planting of transgenic crops with stacked traits. Most of these products have been formed by conventional breeding, i.e. the crossing of transgenic plant (event) containing individual transgenes with other event(s) containing single or double transgenic traits. Many biotech companies are developing stacked trait products with increasing numbers of insect and herbicide tolerance genes for controlling a broad range of insect pests and weeds. There has also been an increase in development of technologies for molecular stacking of multiple traits in a single transgene locus. In this review we look at the status of stacked trait products, crop trait stacking technologies and the technical challenges we are facing. We also review recent progress in developing technology for assembling large transgene arrays in vitro (molecular stacks), their delivery to crop plants and issues they pose for transgene expression.
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Base editing has been utilized for single point mutations and targeted mutation induction for therapeutic corrections of diseases, mutational screening, and gene knockout.
“It has been utilized for several applications, including single point mutations and targeted mutation induction for therapeutic corrections of diseases (Musunuru et al., 2021), mutational screening, and gene knockout among the genomes (Hanna et al., 2021; Que et al., 2010).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Que et al., 2010
Pyramid events, current Bt varieties expressing two or more insecticidal genes, are more effective in controlling target pests and help to slow down the evolution of resistance.
“These so-called pyramid events are more effective in controlling the target pests and help to slow down the evolution of resistance (Gressel et al., 2017;Huang, 2015;Que et al., 2010;Zhao et al. 2005).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Que et al., 2010
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“It has been utilized for several applications, including single point mutations and targeted mutation induction for therapeutic corrections of diseases (Musunuru et al., 2021), mutational screening, and gene knockout among the genomes (Hanna et al., 2021; Que et al., 2010).”
Stacked Bt Proteins Pose No New Risks to Nontarget Arthropods
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- Authors
- Que, Q., Chilton, M.-D. M., de Fontes, C. M., He, C., Nuccio, M., Zhu, T., Wu, Y., Chen, J. S., & Shi, L.
- Affiliations
- Syngenta (United States) (US)
- Venue
- GM Crops
- Publisher
- Informa UK Limited
- Published
- 2010-07-28
- Volume
- 1
- Issue
- 4
- Pages
- 220-229
- ISSN
- 1938-1999 (ISSN-L)
- 1938-2006
- OpenAlex
- W1987126503
- Cited by
- 191
- References
- 0
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 8 · 2023: 8 · 2022: 14 · 2021: 16 · 2020: 18 · 2019: 9 · 2018: 8 · 2017: 18 · 2016: 21 · 2015: 18 · 2014: 13 · 2013: 14 · 2012: 10
- FWCI
- 10.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- GM Crops (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Keywords
- trait stacking, genetically modified crops, molecular stacking, insect tolerance, herbicide tolerance, conventional breeding, transgene expression, transgene delivery
- MeSH terms
- Animals, Breeding, Zea mays, Gossypium, Insecticide Resistance, Insecta, Crops, Agricultural, Transgenes, Plants, Genetically Modified, Disease Resistance
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