Anti-inflammatory effect of aged black garlic on 12-O-tetradecanoylphorbol-13-acetate-induced dermatitis in mice
Open accessAbstract
BACKGROUND/OBJECTIVES:Although aged black garlic has various biological activities such as anti-allergy, anti-inflammation and neuroprotection, effect of aged black garlic on chemically contact dermatitis is unclarified.MATERIALS/METHODS: To evaluate anti-dermatitic activity of aged black garlic extract, we investigated effects of a fraction of aged black garlic extract (BG10) on both in vivo and in vitro.RESULTS: BG10 almost inhibited formation of nitric monoxide and interleukin-6 (IL-6; IC50, 7.07 μg/mL) at 25 μg/mL, and dose-dependently reduced production of tumor necrosis factor-α (TNF-α; IC50, 52.07 μg/mL) and prostaglandin E2 (IC50, 38.46 μg/mL) in lipopolysaccharide-stimulated RAW264.7 cells.In addition, BG10 significantly inhibited the expression of inducible nitric oxide synthase, cyclooxygenase-2 and nuclear NF-κB, and improved that of cytosolic levels of NF-κB and IκBα in the cells.Consistent with in vitro studies, BG10 (0.5 mg/mL) not only reduced ear edema but also suppressed the formation of IL-6 and TNF-α induced by 12-O-tetradecanoylphorbol-13-acetate in ear tissues of mice.CONCLUSIONS: These findings suggest BG10 has anti-dermatitic activity through inhibiting activation of macrophages.Therefore, such effects of BG10 may provide information for the application of aged black garlic for prevention and therapy of contact dermatitis.
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The anti-inflammatory effect of ABG may be attributed to direct suppression of toll-like receptor 4 (TLR4) signaling cascade activation in macrophages, reducing nuclear NF-κB level and improving NF-κB and IκB cytosolic levels in LPS-activated RAW264.7 cells.
“You et al. [74] investigated the anti-inflammatory effect of ABG and they reported that this activity may be attributed to the direct suppression of toll-like receptor 4 (TLR4) signaling cascade activation in macrophages, reducing nuclear NF-κB level and improving the NF-κB and IκB cytosolic levels in LPS-activated RAW264.7 cells.”
From Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review (2020) · cites this paper as [74]
ABG extract may act by inhibiting iNOS and COX-2 expression, and thus prevented NO, interleukin-6 (IL-6) and TNF-α formation in LPS-activated RAW264.7 cells and TPA-mediated dermatitis in mice.
“Additionally, they revealed that ABG extract may act by another mechanism of action by inhibiting the iNOS and COX-2 expression, and thus, prevented the NO, interleukin-6 (IL-6) and TNF-α formation of in LPS-activated RAW264.7 cells and TPA-mediated dermatitis in mice.”
From Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review (2020) · cites this paper as [74]
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- Authors
- You, B. R., Yoo, J.-M., Baek, S. Y., & Kim, M. R.
- Affiliations
- Chungnam National University (KR)
- Korea Institute of Oriental Medicine (KR)
- Department of Food and Nutrition, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea.
- Corresponding author
- Kim, Mee Ree
- Venue
- Nutrition Research and Practice
- Publisher
- XMLink
- Published
- 2019
- Volume
- 13
- Issue
- 3
- Pages
- 189
- ISSN
- 1976-1457 (ISSN-L)
- 2005-6168
- PMC
- PMC6548713
- OpenAlex
- W2948627225
- Cited by
- 35
- References
- 30
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 5 · 2023: 5 · 2022: 8 · 2021: 5 · 2020: 4
- FWCI
- 3.13
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Nutrition Research and Practice (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- Chungnam National University
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Garlic and Onion Studies
- Health Sciences › Medicine › Dermatology › Contact Dermatitis and Allergies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
- Keywords
- contact dermatitis, phorbol myristate acetate, anti-inflammatory activity, macrophage activation, NF-κB signaling, inducible nitric oxide synthase, cyclooxygenase-2, TNF-α, interleukin-6, prostaglandin E2, nitric oxide, RAW 264.7 macrophages, ear edema, IκBα
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