Eugenol Induced Inhibition of Extracellular Enzyme Production by Bacillus subtilis
Open accessAbstract
Eugenol, the major essential oil of clove, in sublethal concentrations (0.02-0.03%, v/v) inhibited the production of alpha-amylase, protease, and subtilisin by Bacillus subtilis in laboratory media. Microscopic observations revealed that at these eugenol concentrations, B. subtilis cells appeared swollen and distorted and/or appeared as very long and thin filaments (> 100 μm). Of 20 amino acids investigated, only L-glutamic or L-aspartic acid (>5.0 mg/ml) prevented such morphogenic distortions when added to eugenol-containing media before inoculation. Addition of these amino acids also resulted in an increase in biomass and protease production. In contrast, the addition of serine (> 1.0 mg/ml) enhanced filamentous growth but reduced the production of protease and subtilisin.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Eugenol acts against different bacterial enzymes, including ATPase, histidine carboxylase, amylase and protease.
“It also acts against different bacterial enzymes, including ATPase, histidine carboxylase, amylase and protease [46,47].”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [46]
Cited by
Sorted by citations
Essential oils: Their antibacterial properties and potential applications in foods—A review
Burt, S. (2004) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.03.022 · Cited by 10,488 · see citing works
“…properties against food borne pathogens contain a high percentage of phenolic compounds such as carvacrol, eugenol (2-methoxy-4-(2-propenyl)phenol) and thymol (Farag et al., 1989; Thoroski et al., 1989; Cosentino et al., 1999; Dorman and Deans, 2000; Juliano et al., 2000; Lambert et al., 2001).”
BackgroundHyldgaard, M., Mygind, T., & Meyer, R. L. (2012) · Frontiers in Microbiology · doi:10.3389/fmicb.2012.00012 · Cited by 2,191 · see citing works
Effect of essential oils on pathogenic bacteria
Nazzaro, F., Fratianni, F., De Martino, L., Coppola, R., & De Feo, V. (2013) · Pharmaceuticals · doi:10.3390/ph6121451 · Cited by 2,186 · see citing works
Swamy, M. K., Akhtar, M. S., & Sinniah, U. R. (2016) · Evidence-Based Complementary and Alternative Medicine · doi:10.1155/2016/3012462 · Cited by 949 · see citing works
Ojagh, S. M., Rezaei, M., Razavi, S. H., & Hosseini, S. M. H. (2010) · Food Chemistry · doi:10.1016/j.foodchem.2010.02.033 · Cited by 866 · see citing works
Essential oils in poultry nutrition: Main effects and modes of action
Brenes, A., & Roura, E. (2010) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2010.03.007 · Cited by 825 · see citing works
Tariq, S., Wani, S., Rasool, W., Shafi, K., Bhat, M. A., Prabhakar, A., Shalla, A. H., & Rather, M. A. (2019) · Microbial Pathogenesis · doi:10.1016/j.micpath.2019.103580 · Cited by 715 · see citing works
Essential Oils as Antimicrobial Agents—Myth or Real Alternative?
Wińska, K., Mączka, W., Łyczko, J., Grabarczyk, M., Czubaszek, A., & Szumny, A. (2019) · Molecules · doi:10.3390/molecules24112130 · Cited by 591 · see citing works
“In another study, it was found that eugenol, the main component of the EO, can inhibit the production of α-amylase, protease, and subtilisin by Bacillus subtilis, with the ability to destroy the cell wall and cause lysis of cells [91] .”
BackgroundUse of Essential Oils in Bioactive Edible Coatings: A Review
Sánchez‐González, L., Vargas, M., González‐Martínez, C., Chiralt, A., & Cháfer, M. (2011) · Food Engineering Reviews · doi:10.1007/s12393-010-9031-3 · Cited by 493 · see citing works
Biological properties of medicinal plants: a review of their antimicrobial activity
Silva, N., & Júnior, A. F. (2010) · The Journal of venomous animals and toxins including tropical diseases · doi:10.1590/s1678-91992010000300006 · Cited by 462 · see citing works
Ksouri, R., Megdiche-Ksouri, W., Jallali, I., Debez, A., Magné, C., Isoda, H., & Abdelly, C. (2011) · Critical Reviews in Biotechnology · doi:10.3109/07388551.2011.630647 · Cited by 437 · see citing works
“Eugenol has been found to inhibit production of amylase and proteases by the sensitive bacteria and to deteriorate cell wall and cause a high degree of cell lysis (Thoroski et al., 1989).”
BackgroundHosseini, S. M. H., Razavi, S., & Mousavi, M. (2009) · Journal of Food Processing and Preservation · doi:10.1111/j.1745-4549.2008.00307.x · Cited by 401 · see citing works
Potential application of essential oils as antimicrobial preservatives in cheese
Khorshidian, N., Yousefi, M., Khanniri, E., & Mortazavian, A. M. (2017) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2017.09.020 · Cited by 262 · see citing works
Development of active packaging film made from poly (lactic acid) incorporated essential oil
Qin, Y., Li, W., Liu, D., et al. (2016) · Progress in Organic Coatings · doi:10.1016/j.porgcoat.2016.10.017 · Cited by 237 · see citing works
Cava-Roda, R. M., Nowak, E., Taboada‐Rodríguez, A., & Marín‐Iniesta, F. (2007) · Journal of Food Protection · doi:10.4315/0362-028x-70.12.2757 · Cited by 176 · see citing works
“Other action mechanisms of clove and cinnamon EOs are based on their capacity to inhibit the production of protease and amylase enzymes (17, 38), inhibit the uptake of glucose (21), and interfere with the proton motive force, electron flow, and active transport (15, 31).”
BackgroundLekjing, S. (2015) · Meat Science · doi:10.1016/j.meatsci.2015.10.003 · Cited by 146 · see citing works
Mittal, R. P., Rana, A., & Jaitak, V. (2018) · Current Drug Targets · doi:10.2174/1389450119666181031122917 · Cited by 149 · see citing works
Boussaada, O., Ammar, S., Saïdana, D., Chriaa, J., Chraif, I., Daami, M., Helal, A. N., & Mighri, Z. (2007) · Microbiological Research · doi:10.1016/j.micres.2007.02.010 · Cited by 131 · see citing works
Saïdana, D., Mahjoub, M. A., Boussaada, O., Chriaa, J., Chéraif, I., Daami, M., Mighri, Z., & Helal, A. N. (2007) · Microbiological Research · doi:10.1016/j.micres.2006.07.009 · Cited by 129 · see citing works
Mechanisms of bactericidal action of Eugenol against Escherichia coli
Jeyakumar, G. E., & Lawrence, R. (2020) · Journal of Herbal Medicine · doi:10.1016/j.hermed.2020.100406 · Cited by 121 · see citing works
Wang, C. Y., Wang, S. Y., Yin, J., Parry, J. W., & Yu, L. L. (2007) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf070429a · Cited by 115 · see citing works
Atanda, O., Akpan, I., & Oluwafemi, F. (2006) · Food Control · doi:10.1016/j.foodcont.2006.02.007 · Cited by 103 · see citing works
Walsh, D. J., Livinghouse, T., Goeres, D. M., Mettler, M. K., & Stewart, P. S. (2019) · Frontiers in Chemistry · doi:10.3389/fchem.2019.00653 · Cited by 104 · see citing works
“cereus, causing degradation of the cell membrane (Thoroski et al., 1989;Wendakoon and Sakaguchi, 1995).”
BackgroundBevilacqua, A., Corbo, M. R., & Sinigaglia, M. (2010) · Journal of Food Protection · doi:10.4315/0362-028x-73.5.888 · Cited by 98 · see citing works
Mohammed, M. J., & Al-Bayati, F. A. Y. (2009) · Phytomedicine · doi:10.1016/j.phymed.2008.12.026 · Cited by 95 · see citing works
Essential Oil Nanoemulsions and their Antimicrobial and Food Applications
Pathania, R., KHAN, H., Kaushik, R., & Khan, M. A. (2018) · Current Research in Nutrition and Food Science Journal · doi:10.12944/crnfsj.6.3.05 · Cited by 97 · see citing works
Potential of rosemary oil to be used in drug-resistant infections.
Luqman, S., Dwivedi, G. R., Darokar, M. P., Kalra, A., & Khanuja, S. P. S. (2007) · PubMed · Cited by 90 · see citing works
The effects of essential oils and their major compounds on fish bacterial pathogens – a review
da Cunha, J. A., Heinzmann, B. M., & Baldisserotto, B. (2018) · Journal of Applied Microbiology · doi:10.1111/jam.13911 · Cited by 80 · see citing works
“…transport, ATP production, and alteration of fatty acid and lipid profiles of bacteria; in addition to direct action on some bacterial enzymes such as carboxylase, protease, ATPase, and amylase as well as bacterial growth (Thoroski et al. 1989; Wendakoon and Sakaguchi 1995; Nazzaro et al. 2013).”
BackgroundAshrafudoulla, M., Mizan, M. F. R., Park, S. H., & Ha, S.-D. (2020) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2020.1767031 · Cited by 77 · see citing works
“Eugenol can disrupt the cytoplasmic membrane and affect ion and ATP transport (Filgueiras and Vanetti 2006; Gill and Holley 2006; Devi et al. 2013), as well as inhibit the growth of pathogenic bacteria (E. coli, Enterobacter aerogenes, and L. monocytogenes) and enzymes such as protease, amylase, and ATPase (Thoroski,…”
BackgroundLee, S., Musa, N., Wee, W., et al. (2009) · Frontiers of Agriculture in China · doi:10.1007/s11703-009-0052-8 · Cited by 74 · see citing works
Valcourt, C., Saulnier, P., Umerska, A. M., Zanelli, M. P., Montagu, A., Rossines, E., & Joly‐Guillou, M. (2015) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2015.11.042 · Cited by 74 · see citing works
Antimicrobial mechanism of nanocellulose composite packaging incorporated with essential oils
Firmanda, A., Fahma, F., Warsiki, E., Syamsu, K., Arnata, I. W., Sartika, D., Suryanegara, L., Qanytah, & Suyanto, A. (2023) · Food Control · doi:10.1016/j.foodcont.2023.109617 · Cited by 69 · see citing works
Saraç, N., & Uğur, A. (2008) · Journal of Medicinal Food · doi:10.1089/jmf.2007.0520 · Cited by 66 · see citing works
Encapsulation and Release of Essential Oils in Functional Silica Nanocontainers
Jobdeedamrong, A., Jenjob, R., & Crespy, D. (2018) · Langmuir · doi:10.1021/acs.langmuir.8b01652 · Cited by 63 · see citing works
Todd, J., Friedman, M., Patel, J., Jaroni, D., & Ravishankar, S. (2013) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2013.06.021 · Cited by 63 · see citing works
Royo, M., Fernández‐Pan, I., & Maté, J. I. (2010) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.3977 · Cited by 62 · see citing works
Isoeugenol has a non-disruptive detergent-like mechanism of action
Hyldgaard, M., Mygind, T., Piotrowska, R., et al. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00754 · Cited by 59 · see citing works
“…intracellular effect on different proteins and enzymes as the structural isomer, eugenol, has been shown to inhibit a myriad of different enzymes such as ATPase, histidine decarboxylase, amylase, and protease (Thoroski et al., 1989; Wendakoon and Morihiko, 1995; Gill and Holley, 2006b).”
BackgroundChemical composition and antibacterial activity of essential oils from six Moroccan plants
Talbaoui, A. (2012) · Journal of Medicinal Plants Research · doi:10.5897/jmpr10.078 · Cited by 53 · see citing works
“When tested at concentration higher than their minimum inhibitory concentration, carvone and eugenol disintegrate the outer membrane (Thoroski et al., 1989; Oosterhaven et al., 1995).”
BackgroundInhibition of Alicyclobacillus acidoterrestris spores by natural compounds
Bevilacqua, A., Corbo, M. R., & Sinigaglia, M. (2007) · International Journal of Food Science & Technology · doi:10.1111/j.1365-2621.2007.01604.x · Cited by 51 · see citing works
Lee, G., Kim, Y., Kim, H., Beuchat, L. R., & Ryu, J. (2017) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2017.11.001 · Cited by 49 · see citing works
Harms, C. A., Ottinger, C. A., & Kennedy‐Stoskopf, S. (2000) · Journal of Aquatic Animal Health · doi:10.1577/1548-8667(2000)012 · Cited by 18 · see citing works
Essential Oils as Alternative Biocides for the Preservation of Waterlogged Archaeological Wood
Antonelli, F., Bartolini, M., Plissonnier, M., Esposito, A., Galotta, G., Ricci, S., Petriaggi, B. D., Pedone, C., Giovanni, A. D., Piazza, S., et al. (2020) · Microorganisms · doi:10.3390/microorganisms8122015 · Cited by 44 · see citing works
Hos, M. H., Razav, S. H., Seyed Mohammad, A., Shahidi, S., & Azade Ghorbani, H. (2008) · Journal of Applied Sciences · doi:10.3923/jas.2008.2895.2900 · Cited by 44 · see citing works
Bahri-Sahloul, R., Fredj, R. B., Boughalleb, N., Shriaa, J., Saguem, S., Hilbert, J., Trotin, F., Ammar, S., Bouzid, S., & Harzallah‐Skhiri, F. (2014) · Journal of Botany · doi:10.1155/2014/623651 · Cited by 43 · see citing works
“The latter has been found to inhibit production of amylase and protease by Bacillus cereus, deteriorate cell wall, and cause cell lysis [51].”
BackgroundStudy on the Antimicrobial activity and Minimum Inhibitory Concentration of Essential Oils of Spices
Babu, A. J., Sundari, A., Indumathi, J., et al. (2011) · Veterinary World · doi:10.5455/vetworld.4.311 · Cited by 42 · see citing works
Antibacterial and Antifungal Activities of the Essential Oils of Two Saltcedar Species from Tunisia
Saïdana, D., Mahjoub, S., Boussaada, O., et al. (2008) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-008-1251-9 · Cited by 40 · see citing works
The ethanopharmacological aspect of carbon nanodots in turmeric smoke
Chun, S., Muthu, M., Gansukh, E., Thalappil, P., & Gopal, J. (2016) · Scientific Reports · doi:10.1038/srep35586 · Cited by 38 · see citing works
Phytochemicals of Plant-Derived Essential Oils
Saroj, A., Oriyomi, O. V., Nayak, A. K., & Haider, S. Z. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-819304-4.00006-3 · Cited by 35 · see citing works
Goldbeck, J. C., Victoria, F. N., da Motta, A. D. S., Savegnago, L., Jacob, R. G., Perin, G., Lenardao, E. J., & da Silva, W. P. (2014) · LWT · doi:10.1016/j.lwt.2014.05.036 · Cited by 35 · see citing works
Elizaquível, P., Sánchez, G., & Aznar, R. (2012) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2012.08.006 · Cited by 35 · see citing works
“…clove and oregano directly affect the membrane permeability due to the presence of high concentrations of eugenol and carvacrol, respectively (Thoroski et al, 1989; Ultee, 2000); cinnamon affects cell metabolism without disintegrating the membrane due to its contents in cynamaldehyde…”
BackgroundResultKeelara, S., Thakur, S., & Patel, J. (2016) · Foodborne Pathogens and Disease · doi:10.1089/fpd.2016.2145 · Cited by 33 · see citing works
WITHDRAWN: Review on antimicrobial packaging containing essential oils and their active biomolecules
Sadaka, F., Nguimjeu, C., Brachais, C., Vroman, I., Tighzert, L., & Couvercelle, J. (2013) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2013.01.004 · Cited by 33 · see citing works
RetractedFeyaerts, J., Rogiers, G., Corthouts, J., & Michiels, C. W. (2014) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2014.12.005 · Cited by 33 · see citing works
Phanthong, P., Lomarat, P., Chomnawang, M. T., & Bunyapraphatsara, N. (2013) · ScienceAsia · doi:10.2306/scienceasia1513-1874.2013.39.472 · Cited by 32 · see citing works
Wang, W., Ren, Z., Wang, L., et al. (2021) · Journal of Food Science · doi:10.1111/1750-3841.15910 · Cited by 31 · see citing works
Goñi, M. G., Tomadoni, B., Moreira, M. R., & Roura, S. I. (2013) · LWT · doi:10.1016/j.lwt.2013.04.021 · Cited by 32 · see citing works
Mouhoub, A., Guendouz, A., Belkamel, A., Talibi, Z. E. A., Koraichi, S. I., Modafar, C. E., & Delattre, C. (2022) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-022-03363-9 · Cited by 29 · see citing works
“In this case, the proposed mode of action is the inactivation of bacterial enzymes such as amylase, proteases, histidine carboxylase, and ATPase (Thoroski et al. 1989; Devi et al. 2010).”
BackgroundMouhoub, A., Guendouz, A., Alaoui-Talibi, Z. E., Koraichi, S. I., & Modafar, C. E. (2025) · Food Biophysics · doi:10.1007/s11483-025-09943-0 · Cited by 27 · see citing works
“The suggested mechanisms of action are the leakage of cell organelles due to the increase in membrane permeability [63], the disruption of cellular functions [67], and the inhibition of the cell enzymes e.g. ATPase, proteases, and amylase [68, 69].”
Antimicrobial activity of essential oils against Escherichia coli O157:H7 in organic soil
Yossa, N., Patel, J., Miller, P. A., & Lo, Y.-M. M. (2010) · Food Control · doi:10.1016/j.foodcont.2010.03.016 · Cited by 24 · see citing works
Karumathil, D. P., Nair, M. S., & Venkitanarayanan, K. (2016) · Phytotherapy Research · doi:10.1002/ptr.5713 · Cited by 23 · see citing works
Essential oils and their components in combating fungal pathogens of animal and human skin
Michalczyk, A. K., & Ostrowska, P. (2021) · Journal de Mycologie Médicale · doi:10.1016/j.mycmed.2021.101118 · Cited by 23 · see citing works
Khorsandi, A., Ziaee, E., Shad, E., et al. (2018) · Journal of Food Protection · doi:10.4315/0362-028x.jfp-17-474 · Cited by 22 · see citing works
Chemical Preservatives and Natural Food Antimicrobials
Taylor, T. M., Ravishankar, S., Bhargava, K., & Juneja, V. K. (2019) · ASM Press eBooks · doi:10.1128/9781555819972.ch27 · Cited by 21 · see citing works
Naddeo, M., Vigliottá, G., Pellecchia, C., & Pappalardo, D. (2020) · Reactive and Functional Polymers · doi:10.1016/j.reactfunctpolym.2020.104714 · Cited by 19 · see citing works
Antimicrobial efficacy of plant essential oils and extracts against Escherichia coli
Gilling, D. H., Ravishankar, S., & Bright, K. R. (2019) · Journal of Environmental Science and Health Part A · doi:10.1080/10934529.2019.1574153 · Cited by 19 · see citing works
Beyond flavor: the versatile roles of eugenol in health and disease
Lao, Y., Guo, J., Fang, J., et al. (2024) · Food & Function · doi:10.1039/d4fo02428a · Cited by 17 · see citing works
Liefferinge, E. V., Forte, C., Degroote, J., Ovyn, A., Noten, N. V., Mangelinckx, S., & Michiels, J. (2022) · Italian Journal of Animal Science · doi:10.1080/1828051x.2022.2041113 · Cited by 17 · see citing works
Neupane, A. C., Sapakuka, S., Tao, P., & Kafle, L. (2020) · International Journal of Pest Management · doi:10.1080/09670874.2019.1641250 · Cited by 16 · see citing works
“ATPase, histidine decarboxylase, amylase, and protease enzymes were each also inhibited by the eugenol (Thoroski et al. 1989; Wendakoon and Sakaguchi 1995; Gill and Holley 2006b).”
BackgroundLomarat, P., Phanthong, P., Wongsariya, K., Chomnawang, M. T., & Bunyapraphatsara, N. (2013) · PubMed · Cited by 16 · see citing works
Wright, M. H., Lee, C. J., Arnold, M. S. J., Shalom, J., White, A. R., Greene, A. C., & Cock, I. E. (2017) · Pharmacognosy Journal · doi:10.5530/pj.2017.5.100 · Cited by 16 · see citing works
Smułek, W., Makiej, A., Jarzębski, M., Zdarta, A., Jeszka‐Skowron, M., Ciesielczyk, F., Jesionowski, T., Zdarta, J., & Kaczorek, E. (2023) · REVIEWS ON ADVANCED MATERIALS SCIENCE · doi:10.1515/rams-2022-0337 · Cited by 16 · see citing works
Tesser, R., Vitiello, R., Russo, V., Turco, R., Di Serio, M., Lin, L., & Li, C. (2020) · Industrial Oil Plant · doi:10.1007/978-981-15-4920-5_8 · Cited by 13 · see citing works
Exploration of Medicinal Plants as Sources of Novel Anticandidal Drugs
Kumar, A., Khan, F., & Saikia, D. (2019) · Current Topics in Medicinal Chemistry · doi:10.2174/1568026619666191025155856 · Cited by 12 · see citing works
Basak, S., Singh, J. K., Morri, S., & Shetty, P. H. (2021) · LWT · doi:10.1016/j.lwt.2021.112076 · Cited by 14 · see citing works
Natural Virucidal Compounds in Foods
Bright, K. R., & Gilling, D. H. (2016) · Springer eBooks · doi:10.1007/978-3-319-30723-7_16 · Cited by 15 · see citing works
“They may cause deterioration of the cell wall (Thoroski et al. 1989 ; Burt 2004 ), damage to cell membranes (Ultee et al. 2002 ) and cell membrane proteins (Ultee et al. 1999 ), increased membrane permeability and leakage of cell contents (Ultee et al. 2002 ; Burt 2004 ), coagulation of cytoplasm…”
BackgroundSuppakul, P. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-800723-5.00039-5 · Cited by 12 · see citing works
Plant Natural Products for Mitigation of Antibiotic Resistance
Ng, W. J., Shit, C. S., Ee, K. Y., & Chai, T. (2021) · Sustainable agriculture reviews · doi:10.1007/978-3-030-58259-3_3 · Cited by 11 · see citing works
Antidiabetic Screening of Phenolic-rich Extracts of Selected Medicinal Spices
Akolade, J. O., Na’Allah, A., Sulyman, A. O., et al. (2018) · Iranian Journal of Science and Technology Transactions A Science · doi:10.1007/s40995-017-0410-y · Cited by 10 · see citing works
Zaineb, T., Uzair, B., Rizg, W. Y., et al. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14122764 · Cited by 10 · see citing works
Interventions for Fresh Produce
Kumar, G. D., Ravishankar, S., & Juneja, V. K. (2017) · Springer eBooks · doi:10.1007/978-1-4939-7556-3_10 · Cited by 10 · see citing works
Antimicrobial efficacy of sequentially applied eugenol against food spoilage micro-organisms
Manrique, Y., Gibis, M., Schmidt, H., & Weiss, J. (2016) · Journal of Applied Microbiology · doi:10.1111/jam.13294 · Cited by 8 · see citing works
“Enzymatic damage without affecting cell division and growth has been demonstrated upon sublethal treatments of Bacillus cereus with eugenol (0 02% v/v) completely inhibiting protease production despite the concomitant increase in biomass (Thoroski et al. 1989).”
BackgroundWITHDRAWN: Review on antimicrobial packaging containing essential oils and their active biomolecules
Sadaka, F., Nguimjeu, C., Brachais, C., Vroman, I., Tighzert, L., & Couvercelle, J. (2014) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2014.03.002 · Cited by 8 · see citing works
RetractedOonmetta-aree, J., & จิราวรรณ, อ. (2005) · Cited by 7 · see citing works
Suárez-Vega, A., Berriozabal, G., de Iriarte, J. P., Lorenzo, J., Álvarez, N., Domínguez-Meister, S., Insausti, S., Rujas, E., Nieva, J. L., Brizuela, M., et al. (2024) · Heliyon · doi:10.1016/j.heliyon.2024.e29146 · Cited by 6 · see citing works
“Eugenol alters the membrane, affects the transport of ions and ATP and changes the fatty acid profile of different bacteria, and acts against different bacterial enzymes, including ATPase, histidine carboxylase, amylase and protease [22,23].”
BackgroundEffects of intramuscularly injected plant-derived antimicrobials in the mouse model
Johnson, E. J., Duan, J. E., Srirattana, K., Venkitanarayanan, K., Tulman, E. R., & Tian, X. (2022) · Scientific Reports · doi:10.1038/s41598-022-09705-9 · Cited by 6 · see citing works
“A total of ten parameters were observed as follows: (1) inflammation of injection site [bump/raise], (2) eating/drinking behavior, (3) levels of activities, (4) overall physical appearance [grooming/fur], (5) body weight Figure 1.”
BackgroundAntimicrobial and Anti-inflammatory Activities of Commercial Aromatizing Fragrances
Bach, H., & Bach, H. (2021) · Future Science OA · doi:10.2144/fsoa-2020-0194 · Cited by 6 · see citing works
Bioactivities and GC-MS profiling of Malewana Madhumeha Choorna polyherbal hot infusion
Keshala, K. K., Bandara, A. M. P. W., Padumadasa, C., & Peiris, L. D. C. (2021) · South African Journal of Botany · doi:10.1016/j.sajb.2021.04.015 · Cited by 6 · see citing works
Essential Oils for the Treatment of Fruit and Vegetables
Barry‐Ryan, C., & Bourke, P. (2012) · doi:10.1002/9781118229187.ch13 · Cited by 9 · see citing works
Bioactivity of Essential Oils Towards Fungi and Bacteria
Bevilacqua, A., Speranza, B., Perricone, M., Sinigaglia, M., & Corbo, M. R. (2017) · Wiley eBooks · doi:10.1002/9781119149392.ch7 · Cited by 6 · see citing works
Clove and its constituents against urban pests: Examples from ants and cockroaches
Kafle, L., & Chinkangsadarn, S. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-85177-0.00026-4 · Cited by 4 · see citing works
Herbal Extracts – Possibility of Preventing Food-Borne Infection
Damjanović-Vratnica, B. (2016) · InTech eBooks · doi:10.5772/62268 · Cited by 4 · see citing works
Effect of essential oils on gut bacteria and functionality in the pig
Michiels, J. (2009) · Ghent University Academic Bibliography (Ghent University) · Cited by 4 · see citing works
Fan, Y., Hui, W., Liu, Q., et al. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.145154 · Cited by 3 · see citing works
Assessment of natural vegetal extracts in the inhibition of Leuconostoc mesenteroides MS1
Serra, M. A., Garnero, J. A., Nicolau, V. V., et al. (2018) · Agriscientia · doi:10.31047/1668.298x.v1.n35.20454 · Cited by 3 · see citing works
Betlej, I., Andres, B. A., Krajewski, K., et al. (2024) · Coatings · doi:10.3390/coatings14040433 · Cited by 2 · see citing works
Antimicrobial Capacities of the Medicinal Halophyte Plants
Medini, F., & Ksouri, R. (2018) · Sustainable development and biodiversity · doi:10.1007/978-3-319-67045-4_11 · Cited by 2 · see citing works
Antibacterial potential of essential oils from medicinal plants for food preservation: a review
Frederico, C., Mendes, S. S., Franciscato, L. M. S. D. S., de Souza, S. C., de Oliveira, K. C., Faria, M. G. I., Gazim, Z. C., & Ruiz, S. P. (2021) · Medicinal Plant Communications · doi:10.37360/mpc.21.4.1.02 · Cited by 2 · see citing works
Non-Conventional Antimicrobial Agents
Pessione, E., & García‐Contreras, R. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-818731-9.00136-1 · Cited by 2 · see citing works
Cinnamaldehyde and Eugenol: Use in Antimicrobial Packaging
Suppakul, P. (2025) · Elsevier eBooks · doi:10.1016/b978-0-323-90747-7.00043-0 · Cited by 1 · see citing works
Leal, P. M. (2013) · Acervo Digital da Universidade Estadual Paulista (Universidade Estadual Paulista) · Cited by 1 · see citing works
- Authors
- Thoroski, J., Blank, G., & Biliaderis, C.
- Affiliations
- University of Manitoba (CA)
- Venue
- Journal of Food Protection
- Publisher
- Elsevier BV
- Published
- 1989-06
- Volume
- 52
- Issue
- 6
- Pages
- 399-403
- ISSN
- 0362-028X (ISSN-L)
- OpenAlex
- W2557933504
- Cited by
- 116
- References
- 0
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 3 · 2023: 4 · 2022: 5 · 2021: 11 · 2020: 7 · 2019: 7 · 2018: 9 · 2017: 6 · 2016: 10 · 2015: 4 · 2014: 4 · 2013: 8 · 2012: 5
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Food Protection (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- eugenol, Bacillus subtilis, extracellular enzyme production, alpha-amylase, proteolytic enzymes, subtilisin, filamentous growth, essential oils, amino acid effects, glutamate, aspartic acid, serine, bacterial morphology
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
