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Cited by 136Cellular transport and secretionHIV Research and TreatmentUbiquitin and proteasome pathways

Structure of cellular ESCRT-III spirals and their relationship to HIV budding

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Cashikar, Anil G, Shim, Soomin, Roth, Robyn, Maldazys, Michael R, Heuser, John E, Hanson, Phyllis I·2014·eLife
DOI 10.7554/elife.02184PMC PMC4073282
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Abstract

The ESCRT machinery along with the AAA+ ATPase Vps4 drive membrane scission for trafficking into multivesicular bodies in the endocytic pathway and for the topologically related processes of viral budding and cytokinesis, but how they accomplish this remains unclear. Using deep-etch electron microscopy, we find that endogenous ESCRT-III filaments stabilized by depleting cells of Vps4 create uniform membrane-deforming conical spirals which are assemblies of specific ESCRT-III heteropolymers. To explore functional roles for ESCRT-III filaments, we examine HIV-1 Gag-mediated budding of virus-like particles and find that depleting Vps4 traps ESCRT-III filaments around nascent Gag assemblies. Interpolating between the observed structures suggests a new role for Vps4 in separating ESCRT-III from Gag or other cargo to allow centripetal growth of a neck constricting ESCRT-III spiral.

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  • Retroviral budding and cytokinesis culminate with a topologically similar membrane abscission process.

    “Both processes of retroviral budding and cytokinesis culminate with a topologically similar membrane abscission process that separates progeny virions from host cell membranes and daughter cells from one another after cell division [13,14,15].”

    From Budding of a Retrovirus: Some Assemblies Required (2020) · cites this paper as [15]

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Research works printed but not linked: 68

  1. Adell MAY Vogel GF Pakdel M Müller M Lindner H Hess MW Teis D 2014 Coordinated binding of Vps4 to ESCRT-III drives membrane neck constriction during MVB vesicle formation. The Journal of Cell Biology 205: 33– 49. doi: 10.1083/jcb.201310114 24711499 PMC3987140 · DOI
  2. Babst M Katzmann DJ Estepa-Sabal EJ Meerloo T Emr SD 2002 Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. Developmental Cell 3: 271– 282. doi: 10.1016/S1534-5807(02)00220-4 12194857 · DOI
  3. Babst M Katzmann D Snyder WB Wendland B Emr SD 2002 Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body. Developmental Cell 3: 283– 289. doi: 10.1016/S1534-5807(02)00219-8 12194858 · DOI
  4. Babst M Wendland B Estepa EJ Emr SD 1998 The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function. The EMBO Journal 17: 2982– 2993. doi: 10.1093/emboj/17.11.2982 9606181 PMC1170638 · DOI
  5. Bachmann L Becker R Leupold G Barth M Guckenberger R Baumeister W 1985 Decoration and shadowing of freeze-etched catalase crystals. Ultramicroscopy 16: 305– 320. doi: 10.1016/0304-3991(85)90099-3 2413603 · DOI
  6. Bajorek M Schubert HL McCullough J Langelier C Eckert DM Stubblefield WM Uter NT Myszka DG Hill CP Sundquist WI 2009 Structural basis for ESCRT-III protein autoinhibition. Nature Structural & Molecular Biology 16: 754– 762. doi: 10.1038/nsmb.1621 PMC2712734 19525971 · DOI
  7. Baumgartel V Ivanchenko S Dupont A Sergeev M Wiseman PW Krausslich HG Brauchle C Muller B Lamb DC 2011 Live-cell visualization of dynamics of HIV budding site interactions with an ESCRT component. Nature Cell Biology 13: 469– 474. doi: 10.1038/ncb2215 21394086 · DOI
  8. Bodon G Chassefeyre R Pernet-Gallay K Martinelli N Effantin G Hulsik DL Belly A Goldberg Y Chatellard-Causse C Blot B Schoehn G Weissenhorn W Sadoul R 2011 Charged multivesicular body protein 2B (CHMP2B) of the endosomal sorting complex required for transport-III (ESCRT-III) polymerizes into helical structures deforming the plasma membrane. The Journal of Biological Chemistry 286: 40276– 40286. doi: 10.1074/jbc.M111.283671 21926173 PMC3220537 · DOI
  9. Boura E Rózycki B Chung H Herrick D Canagarajah B Cafiso D Eaton W Hummer G Hurley J 2012 Solution structure of the ESCRT-I and -II supercomplex: implications for membrane budding and scission. Structure 20: 874– 886. doi: 10.1016/j.str.2012.03.008 22579254 PMC3572543 · DOI
  10. Boura E Różycki B Herrick DZ Chung HS Vecer J Eaton WA Cafiso DS Hummer G Hurley JH 2011 Solution structure of the ESCRT-I complex by small-angle X-ray scattering, EPR, and FRET spectroscopy. Proceedings of the National Academy of Sciences of the United States of America 108: 9437– 9442. doi: 10.1073/pnas.1101763108 21596998 PMC3111319 · DOI
  11. Buchkovich NJ Henne WM Tang S Emr SD 2013 Essential n-terminal insertion motif anchors the ESCRT-III filament during MVB vesicle formation. Developmental Cell 27: 201– 214. doi: 10.1016/j.devcel.2013.09.009 24139821 · DOI
  12. Choudhuri K Llodrá J Roth EW Tsai J Gordo S Wucherpfennig KW Kam LC Stokes DL Dustin ML 2014 Polarized release of T-cell-receptor-enriched microvesicles at the immunological synapse. Nature 507: 118– 123. doi: 10.1038/nature12951 24487619 PMC3949170 · DOI
  13. Davies BA Azmi IF Payne J Shestakova A Horazdovsky BF Babst M Katzmann DJ 2010 Coordination of substrate binding and ATP hydrolysis in Vps4-mediated ESCRT-III disassembly. Molecular Biology of the Cell 21: 3396– 3408. doi: 10.1091/mbc.E10-06-0512 20702581 PMC2947475 · DOI
  14. Dobro MJ Samson RY Yu Z McCullough J Ding HJ Chong PL-G Bell SD Jensen GJ 2013 Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggest spiraling filaments are involved in membrane scission. Molecular Biology of the Cell 24: 2319– 2327. doi: 10.1091/mbc.E12-11-0785 23761076 PMC3727925 · DOI
  15. Effantin G Dordor A Sandrin V Martinelli N Sundquist WI Schoehn G Weissenhorn W 2013 ESCRT-III CHMP2A and CHMP3 form variable helical polymers in vitro and act synergistically during HIV-1 budding. Cellular Microbiology 15: 213– 226. doi: 10.1111/cmi.12041 23051622 PMC3783020 · DOI
  16. Elia N Fabrikant G Kozlov MM Lippincott-Schwartz J 2012 Computational model of cytokinetic abscission driven by ESCRT-III polymerization and remodeling. Biophysical Journal 102: 2309– 2320. doi: 10.1016/j.bpj.2012.04.007 22677384 PMC3353099 · DOI
  17. Elia N Sougrat R Spurlin TA Hurley JH Lippincott-Schwartz J 2011 Dynamics of endosomal sorting complex required for transport (ESCRT) machinery during cytokinesis and its role in abscission. Proceedings of the National Academy of Sciences of the United States of America 108: 4846– 4851. doi: 10.1073/pnas.1102714108 21383202 PMC3064317 · DOI
  18. Fabbro M Zhou BB Takahashi M Sarcevic B Lal P Graham ME Gabrielli BG Robinson PJ Nigg EA Ono Y Khanna KK 2005 Cdk1/Erk2- and Plk1-dependent phosphorylation of a centrosome protein, Cep55, is required for its recruitment to midbody and cytokinesis. Developmental Cell 9: 477– 488. doi: 10.1016/j.devcel.2005.09.003 16198290 · DOI
  19. Fabrikant G Lata S Riches JD Briggs JA Weissenhorn W Kozlov MM 2009 Computational model of membrane fission catalyzed by ESCRT-III. PLOS Computational Biology 5: e1000575. doi: 10.1371/journal.pcbi.1000575 19936052 PMC2773929 · DOI
  20. Fyfe I Schuh AL Edwardson JM Audhya A 2011 Association of the endosomal sorting complex ESCRT-II with the Vps20 subunit of ESCRT-III generates a curvature-sensitive complex capable of nucleating ESCRT-III filaments. The Journal of Biological Chemistry 286: 34262– 34270. doi: 10.1074/jbc.M111.266411 21835927 PMC3190807 · DOI
  21. Garrus JE von Schwedler UK Pornillos O Morham SG Kavitz KH Wang HE Wettstein DA Stray KM Cote MH Rich RL Myszka DG Sundquist WI 2001 Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding. Cell 107: 55– 65. doi: 10.1016/S0092-8674(01)00506-2 11595185 · DOI · Paper page
  22. Ghazi-Tabatabai S Saksena S Short JM Pobbati AV Veprintsev DB Crowther RA Emr SD Egelman EH Williams RL 2008 Structure and disassembly of filaments formed by the ESCRT-III subunit Vps24. Structure 16: 1345– 1356. doi: 10.1016/j.str.2008.06.010 18786397 · DOI
  23. Gheysen D Jacobs E de Foresta F Thiriart C Francotte M Thines D De Wilde M 1989 Assembly and release of HIV-1 precursor Pr55gag virus-like particles from recombinant baculovirus-infected insect cells. Cell 59: 103– 112. doi: 10.1016/0092-8674(89)90873-8 2676191 · DOI
  24. Guizetti J Schermelleh L Mantler J Maar S Poser I Leonhardt H Muller-Reichert T Gerlich DW 2011 Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments. Science 331: 1616– 1620. doi: 10.1126/science.1201847 21310966 · DOI
  25. Gunzenhauser J Olivier N Pengo T Manley S 2012 Quantitative super-resolution imaging reveals protein stoichiometry and nanoscale morphology of assembling HIV-Gag virions. Nano Letters 12: 4705– 4710. doi: 10.1021/nl3021076 22906128 · DOI
  26. Hanson PI Cashikar A 2012 Multivesicular body morphogenesis. Annual Review of Cell and Developmental Biology 28: 337– 362. doi: 10.1146/annurev-cellbio-092910-154152 22831642 · DOI
  27. Hanson PI Roth R Lin Y Heuser JE 2008 Plasma membrane deformation by circular arrays of ESCRT-III protein filaments. The Journal of Cell Biology 180: 389– 402. doi: 10.1083/jcb.200707031 18209100 PMC2213594 · DOI · Paper page
  28. Henne WM Buchkovich NJ Zhao Y Emr SD 2012 The endosomal sorting complex ESCRT-II mediates the assembly and architecture of ESCRT-III helices. Cell 151: 356– 371. doi: 10.1016/j.cell.2012.08.039 23063125 · DOI
  29. Henne WM Stenmark H Emr SD 2013 Molecular mechanisms of the membrane sculpting ESCRT pathway. Cold Spring Harbor Perspectives in Biology 5: a016766. doi: 10.1101/cshperspect.a016766 24003212 PMC3753708 · DOI
  30. Hermida-Matsumoto L Resh MD 2000 Localization of human immunodeficiency virus type 1 Gag and Env at the plasma membrane by confocal imaging. Journal of Virology 74: 8670– 8679. doi: 10.1128/JVI.74.18.8670-8679.2000 10954568 PMC116378 · DOI
  31. Heuser J 2000 The production of ‘cell cortices’ for light and electron microscopy. Traffic 1: 545– 552. doi: 10.1034/j.1600-0854.2000.010704.x 11208142 · DOI
  32. Hurley JH 2010 The ESCRT complexes. Critical Reviews in Biochemistry and Molecular Biology 45: 463– 487. doi: 10.3109/10409238.2010.502516 20653365 PMC2988974 · DOI
  33. Jimenez AJ Maiuri P Lafaurie-Janvore J Divoux S Piel M Perez F 2014 ESCRT machinery is required for plasma membrane repair. Science 343: 1247136. doi: 10.1126/science.1247136 24482116 · DOI
  34. Jouvenet N Zhadina M Bieniasz PD Simon SM 2011 Dynamics of ESCRT protein recruitment during retroviral assembly. Nature Cell Biology 13: 394– 401. doi: 10.1038/ncb2207 PMC3245320 21394083 · DOI
  35. Karacostas V Nagashima K Gonda MA Moss B 1989 Human immunodeficiency virus-like particles produced by a vaccinia virus expression vector. Proceedings of the National Academy of Sciences of the United States of America 86: 8964– 8967. doi: 10.1073/pnas.86.22.8964 2479031 PMC298411 · DOI
  36. Kieffer C Skalicky JJ Morita E De Domenico I Ward DM Kaplan J Sundquist WI 2008 Two distinct modes of ESCRT-III recognition are required for VPS4 functions in lysosomal protein targeting and HIV-1 budding. Developmental Cell 15: 62– 73. doi: 10.1016/j.devcel.2008.05.014 18606141 PMC2586299 · DOI
  37. Lata S Schoehn G Jain A Pires R Piehler J Gottlinger HG Weissenhorn W 2008 Helical structures of ESCRT-III are disassembled by VPS4. Science 321: 1354– 1357. doi: 10.1126/science.1161070 18687924 PMC2758909 · DOI
  38. Lenz M Crow D Joanny J-F 2009 Membrane buckling induced by curved filaments. Physical Review Letters 103: 038101. doi: 10.1103/PhysRevLett.103.038101 19659322 · DOI
  39. Lin Y Kimpler LA Naismith TV Lauer JM Hanson PI 2005 Interaction of the mammalian endosomal sorting complex required for transport (ESCRT) III protein hSnf7-1 with itself, membranes, and the AAA+ ATPase SKD1. The Journal of Biological Chemistry 280: 12799– 12809. doi: 10.1074/jbc.M413968200 15632132 · DOI
  40. Martinelli N Hartlieb B Usami Y Sabin C Dordor A Miguet N Avilov SV Ribeiro EAJ Gottlinger H Weissenhorn W 2012 CC2D1A Is a regulator of ESCRT-III CHMP4B. Journal of Molecular Biology 419: 75– 88. doi: 10.1016/j.jmb.2012.02.044 22406677 PMC3433253 · DOI
  41. McCullough J Colf LA Sundquist WI 2013 Membrane fission reactions of the mammalian ESCRT pathway. Annual Review of Biochemistry 82: 663– 692. doi: 10.1146/annurev-biochem-072909-101058 PMC4047973 23527693 · DOI
  42. Morita E Colf LA Karren MA Sandrin V Rodesch CK Sundquist WI 2010 Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance. Proceedings of the National Academy of Sciences of the United States of America 107: 12889– 12894. doi: 10.1073/pnas.1005938107 20616062 PMC2919903 · DOI
  43. Morita E Sandrin V McCullough J Katsuyama A Baci Hamilton I Sundquist WI 2011 ESCRT-III protein requirements for HIV-1 budding. Cell Host & Microbe 9: 235– 242. doi: 10.1016/j.chom.2011.02.004 21396898 PMC3070458 · DOI · Paper page
  44. Murk JLAN Humbel BM Ziese U Griffith JM Posthuma G Slot JW Koster AJ Verkleij AJ Geuze HJ Kleijmeer MJ 2003 Endosomal compartmentalization in three dimensions: implications for membrane fusion. Proceedings of the National Academy of Sciences of the United States of America 100: 13332– 13337. doi: 10.1073/pnas.2232379100 14597718 PMC263806 · DOI
  45. Muziol T Pineda-Molina E Ravelli RB Zamborlini A Usami Y Gottlinger H Weissenhorn W 2006 Structural basis for budding by the ESCRT-III factor CHMP3. Developmental Cell 10: 821– 830. doi: 10.1016/j.devcel.2006.03.013 16740483 · DOI
  46. Nabhan JF Hu R Oh RS Cohen SN Lu Q 2012 Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein. Proceedings of the National Academy of Sciences of the United States of America 109: 4146– 4151. doi: 10.1073/pnas.1200448109 22315426 PMC3306724 · DOI
  47. Nickerson DP West M Odorizzi G 2006 Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes. The Journal of Cell Biology 175: 715– 720. doi: 10.1083/jcb.200606113 17130288 PMC2064671 · DOI
  48. Peel S Macheboeuf P Martinelli N Weissenhorn W 2011 Divergent pathways lead to ESCRT-III-catalyzed membrane fission. Trends in Biochemical Sciences 36: 199– 210. doi: 10.1016/j.tibs.2010.09.004 21030261 · DOI
  49. Pires R Hartlieb B Signor L Schoehn G Lata S Roessle M Moriscot C Popov S Hinz A Jamin M Boyer V Sadoul R Forest E Svergun DI Gottlinger HG Weissenhorn W 2009 A crescent-shaped ALIX dimer targets ESCRT-III CHMP4 filaments. Structure 17: 843– 856. doi: 10.1016/j.str.2009.04.007 19523902 PMC2699623 · DOI
  50. Pornillos O Higginson DS Stray KM Fisher RD Garrus JE Payne M He GP Wang HE Morham SG Sundquist WI 2003 HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein. The Journal of Cell Biology 162: 425– 434. doi: 10.1083/jcb.200302138 12900394 PMC2172688 · DOI
  51. Saksena S Wahlman J Teis D Johnson AE Emr SD 2009 Functional reconstitution of ESCRT-III assembly and disassembly. Cell 136: 97– 109. doi: 10.1016/j.cell.2008.11.013 19135892 PMC4104304 · DOI
  52. Samson RY Obita T Hodgson B Shaw MK Chong PL Williams RL Bell SD 2011 Molecular and structural basis of ESCRT-III recruitment to membranes during archaeal cell division. Molecular Cell 41: 186– 196. doi: 10.1016/j.molcel.2010.12.018 21255729 PMC3763469 · DOI
  53. Shiels A Bennett TM Knopf HL Yamada K Yoshiura K Niikawa N Shim S Hanson PI 2007 CHMP4B, a novel gene for autosomal dominant cataracts linked to chromosome 20q. American Journal of Human Genetics 81: 596– 606. doi: 10.1086/519980 17701905 PMC1950844 · DOI
  54. Shim S Kimpler LA Hanson PI 2007 Structure/function analysis of four core ESCRT-III proteins reveals common regulatory role for extreme C-terminal domain. Traffic 8: 1068– 1079. doi: 10.1111/j.1600-0854.2007.00584.x 17547705 · DOI
  55. Stein JM Luzio JP 1991 Ectocytosis caused by sublytic autologous complement attach on human neutrophils. The Biochemical Journal 274: 381– 386 1848755 PMC1150148
  56. Strack B Calistri A Craig S Popova E Gottlinger H 2003 AIP1/Alix is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding. Cell 114: 689– 699. doi: 10.1016/S0092-8674(03)00653-6 14505569 · DOI · Paper page
  57. Sundquist WI Krausslich HG 2012 HIV-1 assembly, budding, and maturation. Cold Spring Harbor Perspectives in Medicine 2: a006924. doi: 10.1101/cshperspect.a006924 22762019 PMC3385941 · DOI · Paper page
  58. Teis D Saksena S Emr SD 2008 Ordered assembly of the ESCRT-III complex on endosomes is required to sequester cargo during MVB formation. Developmental Cell 15: 578– 589. doi: 10.1016/j.devcel.2008.08.013 18854142 · DOI
  59. Teis D Saksena S Judson BL Emr SD 2010 ESCRT-II coordinates the assembly of ESCRT-III filaments for cargo sorting and multivesicular body vesicle formation. The EMBO Journal 29: 871– 883. doi: 10.1038/emboj.2009.408 20134403 PMC2837172 · DOI
  60. Thery C 2011 Exosomes: secreted vesicles and intercellular communications. F1000 Biology Reports 3: 15. doi: 10.3410/B3-15 21876726 PMC3155154 · DOI · Paper page
  61. Van Engelenburg SB Shtengel G Sengupta P Waki K Jarnik M Ablan SD Freed EO Hess HF Lippincott-Schwartz J 2014 Distribution of ESCRT machinery at HIV assembly sites reveals virus scaffolding of ESCRT subunits. Science 343: 653– 656. doi: 10.1126/science.1247786 24436186 PMC4791044 · DOI
  62. von Schwedler UK Stuchell M Müller B Ward DM Chung H-Y Morita E Wang HE Davis T He G-P Cimbora DM Scott A Kräusslich H-G Kaplan J Morham SG Sundquist WI 2003 The protein network of HIV budding. Cell 114: 701– 713. doi: 10.1016/S0092-8674(03)00714-1 14505570 · DOI
  63. Wemmer M Azmi I West M Davies B Katzmann D Odorizzi G 2011 Bro1 binding to Snf7 regulates ESCRT-III membrane scission activity in yeast. The Journal of Cell Biology 192: 295– 306. doi: 10.1083/jcb.201007018 21263029 PMC3172170 · DOI
  64. Wollert T Hurley JH 2010 Molecular mechanism of multivesicular body biogenesis by ESCRT complexes. Nature 464: 864– 869. doi: 10.1038/nature08849 20305637 PMC2851844 · DOI · Paper page
  65. Wollert T Wunder C Lippincott-Schwartz J Hurley JH 2009 Membrane scission by the ESCRT-III complex. Nature 458: 172– 177. doi: 10.1038/nature07836 19234443 PMC2743992 · DOI
  66. Wright ER Schooler JB Ding HJ Kieffer C Fillmore C Sundquist WI Jensen GJ 2007 Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells. The EMBO Journal 26: 2218– 2226. doi: 10.1038/sj.emboj.7601664 17396149 PMC1852790 · DOI · Paper page
  67. Xiao J Chen XW Davies B Saltiel AR Katzmann D Xu Z 2009 Structural basis of Ist1 function and Ist1-Did2 interaction in the multivesicular body pathway and cytokinesis. Molecular Biology of the Cell 20: 3514– 3524. doi: 10.1091/mbc.E09-05-0403 19477918 PMC2719569 · DOI
  68. Zamborlini A Usami Y Radoshitzky SR Popova E Palu G Gottlinger H 2006 Release of autoinhibition converts ESCRT-III components into potent inhibitors of HIV-1 budding. Proceedings of the National Academy of Sciences of the United States of America 103: 19140– 19145. doi: 10.1073/pnas.0603788103 17146056 PMC1748189 · DOI

Cited by

Sorted by citations

Authors
Cashikar, A. G., Shim, S., Roth, R., Maldazys, M. R., Heuser, J. E., & Hanson, P. I.
Affiliations
Washington University in St. Louis (US)
Corresponding author
Hanson, Phyllis I
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2014-05-30
Volume
3
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.02184
PMC
PMC4073282
OpenAlex
W2161112640
Cited by
136
References
3
Citations by year
2026: 3 · 2025: 8 · 2024: 8 · 2023: 14 · 2022: 8 · 2021: 10 · 2020: 14 · 2019: 17 · 2018: 14 · 2017: 6 · 2016: 11 · 2015: 18 · 2014: 5
FWCI
6.64
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Digital Commons@Becker (Washington University School of Medicine) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Institutes of Health (R01 GM076686)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
Keywords
ESCRT-III, VPS4, HIV-1 budding, membrane fission, Gag, virus-like particles, multivesicular bodies, cytokinesis, AAA+ ATPase
MeSH terms
Animals, Biological Transport, Cell Membrane, Chlorocebus aethiops, Cytoplasm, HeLa Cells, Humans, Microscopy, Electron, Microscopy, Fluorescence, Polymers, Protein Conformation, HIV-1, Gene Products, gag, COS Cells, Cytokinesis, Endosomal Sorting Complexes Required for Transport, Virus Release, HEK293 Cells

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