The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.
Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans
Open accessAbstract
RNA granules have been likened to liquid droplets whose dynamics depend on the controlled dissolution and condensation of internal components. The molecules and reactions that drive these dynamics in vivo are not well understood. In this study, we present evidence that a group of intrinsically disordered, serine-rich proteins regulate the dynamics of P granules in C. elegans embryos. The MEG (maternal-effect germline defective) proteins are germ plasm components that are required redundantly for fertility. We demonstrate that MEG-1 and MEG-3 are substrates of the kinase MBK-2/DYRK and the phosphatase PP2APPTR−½. Phosphorylation of the MEGs promotes granule disassembly and dephosphorylation promotes granule assembly. Using lattice light sheet microscopy on live embryos, we show that GFP-tagged MEG-3 localizes to a dynamic domain that surrounds and penetrates each granule. We conclude that, despite their liquid-like behavior, P granules are non-homogeneous structures whose assembly in embryos is regulated by phosphorylation.
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2 retracted
mRNP granules: Assembly, function, and connections with disease
Buchan, J. R. (2014) · RNA Biology · doi:10.4161/15476286.2014.972208 · Cited by 459
RetractedBuchan, J. R. (2014) · RNA Biology · doi:10.4161/rna.29034 · Cited by 17
RetractedTHE GENETICS OF CAENORHABDITIS ELEGANS
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Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation
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Phase transitions in the assembly of multivalent signalling proteins
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Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
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Self-citationSpecific interference by ingested dsRNA
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Compositional Control of Phase-Separated Cellular Bodies
Banani, S. F., Rice, A. M., Peeples, W. B., Lin, Y., Jain, S., Parker, R., & Rosen, M. K. (2016) · Cell · doi:10.1016/j.cell.2016.06.010 · Cited by 1,246
A framework for understanding the functions of biomolecular condensates across scales
Lyon, A. S., Peeples, W. B., & Rosen, M. K. (2021) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-00303-z · Cited by 895
RNA Controls PolyQ Protein Phase Transitions
Zhang, H., Elbaum-Garfinkle, S., Langdon, E. M., Taylor, N., Occhipinti, P., Bridges, A. A., Brangwynne, C. P., & Gladfelter, A. S. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.09.017 · Cited by 709
Metadata
- Authors
- Wang, J. T., Smith, J., Chen, B.-C., Schmidt, H., Rasoloson, D., Paix, A., Lambrus, B. G., Calidas, D., Betzig, E., & Seydoux, G.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2014-12-23
- Volume
- 3
- Pages
- e04591-e04591
- ISSN
- 2050-084X (ISSN-L)
- OpenAlex
- W2104873074
- Cited by
- 364
- References
- 57
- FWCI
- 10.59
- Type
- journal article
- Language
- en
- Open access
- gold
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- Wang, J. T., Smith, J., Chen, B.-C., Schmidt, H., Rasoloson, D., Paix, A., Lambrus, B. G., Calidas, D., Betzig, E., & Seydoux, G. (2014). Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans. eLife. https://doi.org/10.7554/elife.04591
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