The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Bioengineered human myobundles mimic clinical responses of skeletal muscle to drugs
Open accessAbstract
Existing in vitro models of human skeletal muscle cannot recapitulate the organization and function of native muscle, limiting their use in physiological and pharmacological studies. Here, we demonstrate engineering of electrically and chemically responsive, contractile human muscle tissues (‘myobundles’) using primary myogenic cells. These biomimetic constructs exhibit aligned architecture, multinucleated and striated myofibers, and a Pax7 + cell pool. They contract spontaneously and respond to electrical stimuli with twitch and tetanic contractions. Positive correlation between contractile force and GCaMP6-reported calcium responses enables non-invasive tracking of myobundle function and drug response. During culture, myobundles maintain functional acetylcholine receptors and structurally and functionally mature, evidenced by increased myofiber diameter and improved calcium handling and contractile strength. In response to diversely acting drugs, myobundles undergo dose-dependent hypertrophy or toxic myopathy similar to clinical outcomes. Human myobundles provide an enabling platform for predictive drug and toxicology screening and development of novel therapeutics for muscle-related disorders.
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Chen, T.-W., Wardill, T. J., Sun, Y., Pulver, S. R., Renninger, S. L., Baohan, A., Schreiter, E. R., Kerr, R. A., Orger, M. B., Jayaraman, V., et al. (2013) · Nature · doi:10.1038/nature12354 · Cited by 6,474
Muscles, exercise and obesity: skeletal muscle as a secretory organ
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Reducing safety-related drug attrition: the use of in vitro pharmacological profiling
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Self-citationSalva, M. Z., Himeda, C. L., Tai, P. W., Nishiuchi, E., Gregorevic, P., Allen, J. M., Finn, E. E., Nguyen, Q. G., Blankinship, M. J., Meuse, L., et al. (2007) · Molecular Therapy · doi:10.1038/sj.mt.6300027 · Cited by 232
Phenotypic screening of the ToxCast chemical library to classify toxic and therapeutic mechanisms
Kleinstreuer, N. C., Yang, J., Berg, E. L., Knudsen, T. B., Richard, A. M., Martin, M. T., Reif, D. M., Judson, R. S., Polokoff, M., Dix, D. J., et al. (2014) · Nature Biotechnology · doi:10.1038/nbt.2914 · Cited by 211
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Self-citationEngineering human pluripotent stem cells into a functional skeletal muscle tissue
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Self-citationAfshar Bakooshli, M., Lippmann, E. S., Mulcahy, B., Iyer, N., Nguyen, C. T., Tung, K., Stewart, B. A., van den Dorpel, H., Fuehrmann, T., Shoichet, M., et al. (2019) · eLife · doi:10.7554/elife.44530 · Cited by 268
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Metadata
- Authors
- Madden, L., Juhas, M., Kraus, W. E., Truskey, G. A., & Bursac, N.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2015-01-09
- Volume
- 4
- Pages
- e04885-e04885
- ISSN
- 2050-084X (ISSN-L)
- OpenAlex
- W2171103133
- Cited by
- 356
- References
- 6
- FWCI
- 12.87
- Type
- journal article
- Language
- en
- Open access
- gold
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- Madden, L., Juhas, M., Kraus, W. E., Truskey, G. A., & Bursac, N. (2015). Bioengineered human myobundles mimic clinical responses of skeletal muscle to drugs. eLife. https://doi.org/10.7554/elife.04885
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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