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Cited by 60Zebrafish Biomedical Research Applicationsmelanin and skin pigmentationDevelopmental Biology and Gene Regulation

Tight Junction Protein 1a regulates pigment cell organisation during zebrafish colour patterning

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Fadeev, Andrey, Krauss, Jana, Frohnhöfer, Hans Georg, Irion, Uwe, Nüsslein-Volhard, Christiane·2015·eLife
DOI 10.7554/elife.06545PMC PMC4446668
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Abstract

Zebrafish display a prominent pattern of alternating dark and light stripes generated by the precise positioning of pigment cells in the skin. This arrangement is the result of coordinated cell movements, cell shape changes, and the organisation of pigment cells during metamorphosis. Iridophores play a crucial part in this process by switching between the dense form of the light stripes and the loose form of the dark stripes. Adult schachbrett (sbr) mutants exhibit delayed changes in iridophore shape and organisation caused by truncations in Tight Junction Protein 1a (ZO-1a). In sbr mutants, the dark stripes are interrupted by dense iridophores invading as coherent sheets. Immuno-labelling and chimeric analyses indicate that Tjp1a is expressed in dense iridophores but down-regulated in the loose form. Tjp1a is a novel regulator of cell shape changes during colour pattern formation and the first cytoplasmic protein implicated in this process.

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  • tjp1a is highly expressed only in dense iridophores (found in the interstripe), and knocking out tjp1a results in disruption of the stripe pattern due to dense (interstripe) iridophores invading the stripe regions.

    “it was recently shown using immuno-labeling that Tight Junction Protein 1a (tjp1a) is highly expressed only in dense iridophores19 (found in the interstripe), and that knocking out tjp1a results in the disruption of the stripe pattern due to dense (interstripe) iridophores invading the stripe regions19”

    From In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning (2020) · cites this paper as [19]

    The Results section reports antibody staining in dense iridophores of the light stripe, but not in loose iridophores or other pigment cells. The Discussion reports that Tjp1a-deficient mutants develop dark-stripe interruptions as dense iridophores invade those regions. The unqualified claim that Tjp1a is expressed only in dense iridophores goes beyond the paper: the Results also detect it in epithelial cells and blood vessels. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • The apparent conflict between the authors' observations and those of prior studies may reflect differences in how iridophores and their progenitors have been visualized.

    “The apparent conflict between our observations and those of prior studies5,12,19 may reflect differences in how iridophores and their progenitors have been visualized”

    From In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning (2020) · cites this paper as [19]

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Cited by

Sorted by citations

Authors
Fadeev, A., Krauss, J., Frohnhöfer, H. G., Irion, U., & Nüsslein-Volhard, C.
Affiliations
  • Max Planck Institute for Developmental Biology (DE)
  • Max Planck Institute for Developmental Biology, Tübingen, Germany
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2015-04-27
Volume
4
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.06545
PMC
PMC4446668
OpenAlex
W1987442025
Cited by
60
References
0
Citations by year
2026: 2 · 2025: 5 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 10 · 2020: 4 · 2019: 8 · 2018: 6 · 2017: 11 · 2016: 7 · 2015: 1
FWCI
4.00
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Max-Planck-Gesellschaft (Max Planck Society)
  • European Commission (EC) (ZF-HEALTH grant (Project number 242048))
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
iridophores, metamorphosis, cell shape changes, cell motility, chimeric analysis, skin pigmentation
MeSH terms
Animals, Cell Movement, Metamorphosis, Biological, Pigmentation, Zebrafish, Body Patterning, Zebrafish Proteins, Zonula Occludens-1 Protein

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