Eco-HAB as a fully automated and ecologically relevant assessment of social impairments in mouse models of autism
Open accessAbstract
Eco-HAB is an open source, RFID-based system for automated measurement and analysis of social preference and in-cohort sociability in mice. The system closely follows murine ethology. It requires no contact between a human experimenter and tested animals, overcoming the confounding factors that lead to irreproducible assessment of murine social behavior between laboratories. In Eco-HAB, group-housed animals live in a spacious, four-compartment apparatus with shadowed areas and narrow tunnels, resembling natural burrows. Eco-HAB allows for assessment of the tendency of mice to voluntarily spend time together in ethologically relevant mouse group sizes. Custom-made software for automated tracking, data extraction, and analysis enables quick evaluation of social impairments. The developed protocols and standardized behavioral measures demonstrate high replicability. Unlike classic three-chambered sociability tests, Eco-HAB provides measurements of spontaneous, ecologically relevant social behaviors in group-housed animals. Results are obtained faster, with less manpower, and without confounding factors.
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In the laboratory, mice are more often than rats kept in large groups (more than 10 animals), which facilitates studying high-order social behaviors such as emergence of social networks.
“Finally, in the laboratory, mice are more often than rats kept in large groups (ie, more than 10 animals), which facilitates studying high-order social behaviors which cannot be reduced to interactions within dyads, for example, emergence of social networks.1,2,72”
From Ecological validity of social interaction tests in rats and mice (2019) · cites this paper as [1]
Some strains of mice are more 'social' than others; some tend to group together more and/or engage in more social interactions.
“There is compelling evidence that some strains are more”
From Ecological validity of social interaction tests in rats and mice (2019) · cites this paper as [1]
Mice are usually harder to habituate, but easier to house in big groups and complex environments.
“Mice are usually harder to habituate, but on the other hand easier to house in big groups and complex environments.1,51”
From Ecological validity of social interaction tests in rats and mice (2019) · cites this paper as [1]
Automated systems for measuring social interactions in group-living animals are becoming more and more popular.
“Such systems are becoming more and more popular.1,2,179”
From Ecological validity of social interaction tests in rats and mice (2019) · cites this paper as [1]
The Eco-HAB system offers an easy and fast way of investigating between-strain and between-genotype differences in sociability in mice.
“The system, which is equipped with software for data acquisition and analysis, offers the easy and fast way of investigating between-strain and between-genotype differences in sociability in mice.1”
From Ecological validity of social interaction tests in rats and mice (2019) · cites this paper as [1]
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Self-citation
- Authors
- Puścian, A., Łęski, S., Kasprowicz, G., Winiarski, M., Borowska, J., Nikolaev, T., Boguszewski, P. M., Lipp, H.-P., & Knapska, E.
- Affiliations
- Instytut Biologii Doświadczalnej im. Marcelego Nenckiego (PL)
- Warsaw University of Technology (PL)
- University of Zurich (CH)
- Corresponding author
- Knapska, Ewelina
- ORCID
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2016-10-12
- Volume
- 5
- ISSN
- 2050-084X (ISSN-L)
- PMC
- PMC5092044
- OpenAlex
- W2530742534
- Cited by
- 69
- References
- 49
- Citations by year
- 2026: 10 · 2025: 7 · 2024: 13 · 2023: 6 · 2022: 11 · 2021: 3 · 2020: 9 · 2019: 5 · 2018: 3 · 2017: 2
- FWCI
- 2.12
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- eLife (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Zurich Open Repository and Archive (University of Zurich) (acceptedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Zurich Open Repository and Archive (University of Zurich) (submittedVersion, other-oa)
- Zurich Open Repository and Archive (University of Zurich)
- Funders
- Swiss Contribution to the enlarged European Union (PSPB-210)
- Narodowe Centrum Nauki (2013/08/W/NZ4/00691)
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Autism Spectrum Disorder Research
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Keywords
- mouse models of autism, social behavior assessment, social preferences, sociability, RFID tracking, automated behavioral analysis, group-housed mice
- MeSH terms
- Animals, Behavior, Animal, Disease Models, Animal, Mice, Inbred BALB C, Mice, Inbred C57BL, Social Behavior Disorders, Reproducibility of Results, Automation, Laboratory
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