Microengineered 3D collagen gels with independently tunable fiber anisotropy and directionality
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Abstract
Abstract Cellular processes are linked to the alignment (anisotropy) and orientation (directionality) of collagen fibers (i.e., landscape) in the native extracellular matrix (ECM). Given the vital role that cell‐matrix interactions play in regulating biological functions, several microfluidic methods have successfully established anisotropic 3D collagen gels to develop quantitative relationships between structural cues and cellular responses. However, independently tailoring the fiber anisotropy and fiber directionality within a landscape remains a challenge. Here, a user‐friendly microfluidic platform with a non‐uniform channel geometry is used to control the degree of fiber anisotropy and directionality as a function of extensional strain rate and a defined flow path, respectively. New experimental capabilities, including independent control over the degree of fiber anisotropy and directionality, spatial gradients in anisotropy, and multi‐material interfaces, are demonstrated. A channel peel‐off technique provides direct access to the microengineered collagen landscapes, and the alignment of single MD‐MB‐231 cancer cells and monolayers of human umbilical vein endothelial cells (HUVEC) is shown. Finally, the platform's modular capability is highlighted by integrating an ultrathin porous Parylene (UPP) membrane onto the microengineered collagen landscape as a method to control the degree of cell‐matrix interaction.
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ECM orientation can be achieved by providing directional forces during or after fibril polymerization, such as external strain, flow, or shear.
“ECM orientation can be further achieved by providing directional forces during or after fibril polymerization, such as by external strain, flow, or shear [21,37,38].”
From Cancer-on-chip models for metastasis: importance of the tumor microenvironment (2024) · cites this paper as [37]
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Self-citation
- Authors
- Ahmed, A., Joshi, I. M., Larson, S., Mansouri, M., Gholizadeh, S., Allahyari, Z., Forouzandeh, F., Borkholder, D. A., Gaborski, T. R., & Abhyankar, V. V.
- Affiliations
- Rochester Institute of Technology (US)
- Corresponding author
- Abhyankar, Vinay V.
- ORCID
- Venue
- Advanced Materials Technologies
- Publisher
- Wiley
- Published
- 2021-03-10
- Volume
- 6
- Issue
- 4
- ISSN
- 2365-709X (ISSN-L)
- PMC
- PMC8211114
- OpenAlex
- W3135154129
- Cited by
- 27
- References
- 59
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 3 · 2023: 6 · 2022: 9 · 2021: 1
- FWCI
- 2.48
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Advanced Materials Technologies (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (R01DC014568, R35GM119623)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- collagen gel, fiber anisotropy, extensional strain rate, cell alignment, human umbilical vein endothelial cells, extracellular matrix, cell-matrix interactions, multi-material interfaces
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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