Engineering fiber anisotropy within natural collagen hydrogels
Open accessAbstract
It is well known that biophysical properties of the extracellular matrix (ECM), including stiffness, porosity, composition, and fiber alignment (anisotropy), play a crucial role in controlling cell behavior in vivo. Type I collagen (collagen I) is a ubiquitous structural component in the ECM and has become a popular hydrogel material that can be tuned to replicate the mechanical properties found in vivo. In this review article, we describe popular methods to create 2-D and 3-D collagen I hydrogels with anisotropic fiber architectures. We focus on methods that can be readily translated from engineering and materials science laboratories to the life-science community with the overall goal of helping to increase the physiological relevance of cell culture assays.
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Efforts have been made to design anisotropic matrix structures in CoC devices, particularly collagen type I, to recapitulate in vivo-like aligned collagen-rich ECM interacting with invasive tumors, using engineered, directional force-derived, or cell-derived approaches.
“More recently, however, efforts have been made to design anisotropic matrix structures (including in CoC devices) in particular, but not exclusively, collagen type I [24], with the aim of recapitulating in vivo-like aligned collagen-rich ECM interacting with invasive tumors [25] by using (i) engineered, (ii) directional force-derived, or (iii) cell-derived approaches (Figure 2A-C and Table 1).”
From Cancer-on-chip models for metastasis: importance of the tumor microenvironment (2024) · cites this paper as [24]
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Self-citation
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Cancer-on-chip models for metastasis: importance of the tumor microenvironment
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- Authors
- Ahmed, A., Joshi, I. M., Mansouri, M., Ahamed, N. N. N., Hsu, M.-C., Gaborski, T. R., & Abhyankar, V. V.
- Affiliations
- Rochester Institute of Technology (US)
- Corresponding author
- Abhyankar, Vinay V.
- ORCID
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2021-06-01
- Volume
- 320
- Issue
- 6
- Pages
- C1112-C1124
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- PMC
- PMC8285641
- OpenAlex
- W3154625788
- Cited by
- 41
- References
- 122
- Citations by year
- 2026: 8 · 2025: 6 · 2024: 9 · 2023: 7 · 2022: 10 · 2021: 1
- FWCI
- 0.85
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by
- Locations
- American Journal of Physiology-Cell Physiology (publishedVersion, cc-by)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- HHS | National Institutes of Health (R35GM119623, R61HL154249, R43GM137651)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Keywords
- collagen I hydrogel, fiber anisotropy, extracellular matrix, type I collagen, 3D cell culture, hydrogel synthesis, cell behavior, hydrogel mechanical properties
- MeSH terms
- Animals, Extracellular Matrix, Humans, Anisotropy, Hydrogels, Tissue Engineering, Collagen Type I
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