Oriented collagen fibers direct tumor cell intravasation
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In this work, we constructed a Collagen I-Matrigel composite extracellular matrix (ECM). The composite ECM was used to determine the influence of the local collagen fiber orientation on the collective intravasation ability of tumor cells. We found that the local fiber alignment enhanced cell-ECM interactions. Specifically, metastatic MDA-MB-231 breast cancer cells followed the local fiber alignment direction during the intravasation into rigid Matrigel (∼10 mg/mL protein concentration).
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ECM orientation can be achieved by providing directional forces during or after fibril polymerization, such as external strain, flow, or shear.
“ECM orientation can be further achieved by providing directional forces during or after fibril polymerization, such as by external strain, flow, or shear [21,37,38].”
From Cancer-on-chip models for metastasis: importance of the tumor microenvironment (2024) · cites this paper as [21]
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Self-citation
- Authors
- Han, W., Chen, S., Yuan, W., Fan, Q., Tian, J., Wang, X., Chen, L., Zhang, X., Wei, W., Liu, R., Qu, J., Jiao, Y., Austin, R. H., & Liu, L.
- Affiliations
- Chinese Academy of Sciences (CN)
- Arizona State University (US)
- Shenzhen University (CN)
- King Abdullah University of Science and Technology (SA)
- Chongqing University (CN)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2016-09-23
- Volume
- 113
- Issue
- 40
- Pages
- 11208-11213
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC5056065
- OpenAlex
- W2464386848
- Cited by
- 376
- References
- 48
- Citations by year
- 2026: 15 · 2025: 42 · 2024: 46 · 2023: 44 · 2022: 51 · 2021: 51 · 2020: 50 · 2019: 41 · 2018: 21 · 2017: 12 · 2016: 1
- FWCI
- 17.13
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- King Abdullah University of Science and Technology Repository (King Abdullah University of Science and Technology) (submittedVersion)
- Funders
- State Key Development Program Basic Research China (2013CB837200)
- National Science Foundation China (11474345, 11274200)
- Beijing Natural Science Foundation (7154221)
- HHS | NIH | National Cancer Institute (U54CA143803)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Keywords
- collagen fiber orientation, tumor cell intravasation, extracellular matrix, cell-matrix interactions, collective cell migration, breast cancer metastasis, MDA-MB-231 cells, Matrigel
- MeSH terms
- Biopsy, Collagen, Extracellular Matrix, Female, Humans, Neoplasm Metastasis, Neoplasms, Disease Progression, Cell Line, Tumor, Cell Proliferation, Time-Lapse Imaging
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