The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
miR‐155 as a multiple sclerosis–relevant regulator of myeloid cell polarization
Open accessAbstract
Objective: To define the functional significance of increased miR‐155 expression in myeloid cells in multiple sclerosis (MS). Methods: miR‐155 expression levels were measured in CD14 + monocytes from untreated relapsing–remitting MS patients and compared to healthy controls. Similar microRNA (miRNA) analyses were performed in laser‐captured CD68 + cells from perivascular (blood‐derived macrophages) and parenchymal (microglia) brain regions in both active MS lesions and noninflammatory cases. Using human adult blood‐derived macrophages and brain‐derived microglia, in vitro experiments were performed to demonstrate how miR‐155 influences the polarization state, phenotype, and functional properties of myeloid cells, in addition to their ability to subsequently impact adaptive T‐cell responses. Results: In MS, miR‐155 expression was significantly increased in both peripheral circulating CD14 + monocytes and active lesions (CD68 + cells) compared to control donor monocytes and parenchymal microglia, respectively. In vitro, miR‐155 was significantly increased in both M1‐polarized primary human macrophages and microglia. Transfection of an miR‐155 mimic increased proinflammatory cytokine secretion and costimulatory surface marker expression in both cell types; an miR‐155 inhibitor decreased proinflammatory cytokine expression. Coculture experiments demonstrated that allogeneic T‐cell responses were significantly enhanced in the presence of miR‐155–transfected myeloid cells compared to controls. Interpretation: Our results demonstrate that miR‐155 regulates proinflammatory responses in both blood‐derived and central nervous system (CNS)‐resident myeloid cells, in addition to impacting subsequent adaptive immune responses. Differential miRNA expression may therefore provide insight into mechanisms responsible for distinct phenotypic and functional properties of myeloid cells, thus impacting their ability to influence CNS injury and repair. Ann Neurol 2013;74:709–720
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“Also, since many disorders share the same proteins or micro RNAs as markers, it is impossible to have selective detection of diseases [313–317].”
From Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions (2024) · cites this paper as [314]
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“MiR-155 is upregulated in M1-polarized macrophages and microglia, and in those cells in patients with multiple sclerosis [248].”
From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [248]
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Microglial and macrophage polarization—new prospects for brain repair
Hu, X., Leak, R. K., Shi, Y., Suenaga, J., Gao, Y., Zheng, P., & Chen, J. (2015) · Nature Reviews Neurology · doi:10.1038/nrneurol.2014.207 · Cited by 1,366
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Self-citationMicroglia and brain macrophages in the molecular age: from origin to neuropsychiatric disease
Prinz, M., & Priller, J. (2014) · Nature Reviews Neuroscience · doi:10.1038/nrn3722 · Cited by 1,329
The biphasic function of microglia in ischemic stroke
Ma, Y., Wang, J., Wang, Y., & Yang, G.-Y. (2017) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2016.01.005 · Cited by 655
“Increasing evidence revealed that microRNAs play an important role in the pathophysiology of CNS diseases, including AD, Parkinson’s disease (PD), EAE, multiple sclerosis (MS), ALS, and stroke (Butovsky et al., 2012; Junn and Mouradian, 2012; Moore et al., 2013; Parisi et al., 2013; Ponomarev et al., 2011; Sun et al.,…”
Essandoh, K., Li, Y., Huo, J., & Fan, G. (2016) · Shock · doi:10.1097/shk.0000000000000604 · Cited by 610
“Polarized macrophages interact with various tissues and organs in diseases such as obesity (81–86), cancer (62–65), and multiple sclerosis (87, 88), thus affecting pathophysiology of such disorders.”
Background
Metadata
- Authors
- Moore, C. S., Rao, V. T. S., Durafourt, B. A., Bedell, B. J., Ludwin, S. K., Bar‐Or, A., & Antel, J. P.
- Affiliations
- Montreal Neurological Institute and Hospital (CA)
- Queen's University (CA)
- Corresponding author
- Moore, Craig S.
- Venue
- Annals of Neurology
- Publisher
- Wiley
- Published
- 2013-09-23
- Volume
- 74
- Issue
- 5
- Pages
- 709-720
- ISSN
- 0364-5134 (ISSN-L)
- 1531-8249
- OpenAlex
- W1915908724
- Cited by
- 219
- References
- 48
- Citations by year
- 2026: 8 · 2025: 6 · 2024: 13 · 2023: 11 · 2022: 13 · 2021: 15 · 2020: 25 · 2019: 26 · 2018: 23 · 2017: 24 · 2016: 23 · 2015: 15 · 2014: 15 · 2013: 2
- FWCI
- 13.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Annals of Neurology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Clinical Neurology › Multiple Sclerosis Research Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- miR-155, multiple sclerosis, myeloid cell polarization, microglia, blood-derived macrophages, CD14+ monocytes, M1 macrophage polarization, inflammatory cytokines, costimulatory molecules, T cell response, relapsing-remitting multiple sclerosis, neuroinflammation
- MeSH terms
- Adult, Aged, Brain, Female, Humans, Macrophages, Male, Middle Aged, Multiple Sclerosis, T-Lymphocytes, Cell Polarity, Microglia, Myeloid Cells, MicroRNAs, Cell Proliferation, Adaptive Immunity
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