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miR‐155 as a multiple sclerosis–relevant regulator of myeloid cell polarization
Open accessAbstract
Objective: To define the functional significance of increased miR‐155 expression in myeloid cells in multiple sclerosis (MS). Methods: miR‐155 expression levels were measured in CD14 + monocytes from untreated relapsing–remitting MS patients and compared to healthy controls. Similar microRNA (miRNA) analyses were performed in laser‐captured CD68 + cells from perivascular (blood‐derived macrophages) and parenchymal (microglia) brain regions in both active MS lesions and noninflammatory cases. Using human adult blood‐derived macrophages and brain‐derived microglia, in vitro experiments were performed to demonstrate how miR‐155 influences the polarization state, phenotype, and functional properties of myeloid cells, in addition to their ability to subsequently impact adaptive T‐cell responses. Results: In MS, miR‐155 expression was significantly increased in both peripheral circulating CD14 + monocytes and active lesions (CD68 + cells) compared to control donor monocytes and parenchymal microglia, respectively. In vitro, miR‐155 was significantly increased in both M1‐polarized primary human macrophages and microglia. Transfection of an miR‐155 mimic increased proinflammatory cytokine secretion and costimulatory surface marker expression in both cell types; an miR‐155 inhibitor decreased proinflammatory cytokine expression. Coculture experiments demonstrated that allogeneic T‐cell responses were significantly enhanced in the presence of miR‐155–transfected myeloid cells compared to controls. Interpretation: Our results demonstrate that miR‐155 regulates proinflammatory responses in both blood‐derived and central nervous system (CNS)‐resident myeloid cells, in addition to impacting subsequent adaptive immune responses. Differential miRNA expression may therefore provide insight into mechanisms responsible for distinct phenotypic and functional properties of myeloid cells, thus impacting their ability to influence CNS injury and repair. Ann Neurol 2013;74:709–720
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“Also, since many disorders share the same proteins or micro RNAs as markers, it is impossible to have selective detection of diseases [313–317].”
From Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions (2024) · cites this paper as [314]
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“MiR-155 is upregulated in M1-polarized macrophages and microglia, and in those cells in patients with multiple sclerosis [248].”
From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [248]
Citations
Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
Taganov, K. D., Boldin, M. P., Chang, K.-J., & Baltimore, D. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0605298103 · Cited by 4,317
Identification of Mammalian microRNA Host Genes and Transcription Units
Rodriguez, A., Griffiths-Jones, S., Ashurst, J. L., & Bradley, A. (2004) · Genome Research · doi:10.1101/gr.2722704 · Cited by 2,128
Requirement of bic/microRNA-155 for Normal Immune Function
Rodriguez, A., Vigorito, E., Clare, S., Warren, M. V., Couttet, P., Soond, D. R., van Dongen, S., Grocock, R. J., Das, P. P., Miska, E. A., et al. (2007) · Science · doi:10.1126/science.1139253 · Cited by 1,833
Regulation of the Germinal Center Response by MicroRNA-155
Thai, T., Calado, D. P., Casola, S. S., Ansel, K. M., Xiao, C., Xue, Y., Murphy, A. J., Frendewey, D. A., Valenzuela, D. M., Kutok, J. L., et al. (2007) · Science · doi:10.1126/science.1141229 · Cited by 1,477
Cogswell, J. P., Ward, J., Taylor, I. A., Waters, M., Shi, Y., Cannon, B., Kelnar, K., Kemppainen, J., Brown, D., Chen, C., et al. (2008) · Journal of Alzheimer s Disease · doi:10.3233/jad-2008-14103 · Cited by 943
Ponomarev, E. D., Veremeyko, T., Barteneva, N., Krichevsky, A. M., & Weiner, H. L. (2011) · Nature Medicine · doi:10.1038/nm.2266 · Cited by 787
Hou, J., Wang, P., Lin, L., Liu, X., Ma, F., An, H., Wang, Z., & Cao, X. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.0900707 · Cited by 739
TLR signaling tailors innate immune responses in human microglia and astrocytes
Jack, C. S., Arbour, N., Manusow, J., Montgrain, V., Blain, M., McCrea, E., Shapiro, A., & Antel, J. P. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.175.7.4320 · Cited by 734
Self-citationJunker, A., Krumbholz, M., Eisele, S., Mohan, H., Augstein, F., Bittner, R., Lassmann, H., Wekerle, H., Hohlfeld, R., & Meinl, E. (2009) · Brain · doi:10.1093/brain/awp300 · Cited by 595
Comparison of polarization properties of human adult microglia and blood‐derived macrophages
Durafourt, B. A., Moore, C. S., Zammit, D., Johnson, T., Zaguia, F., Guiot, M., Bar‐Or, A., & Antel, J. P. (2012) · Glia · doi:10.1002/glia.22298 · Cited by 470
Self-citationIdentifying Functional MicroRNAs in Macrophages with Polarized Phenotypes
Graff, J. W., Dickson, A. M., Clay, G., McCaffrey, A. P., & Wilson, M. E. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.327031 · Cited by 367
Keller, A., Leidinger, P., Lange, J., Borries, A., Schrörs, H., Scheffler, M., Lenhof, H., Ruprecht, K., & Meese, E. U. (2009) · PLoS ONE · doi:10.1371/journal.pone.0007440 · Cited by 365
Cardoso, A. L., Guedes, J. R., Pereira de Almeida, L., & Pedroso de Lima, M. C. (2012) · Immunology · doi:10.1111/j.1365-2567.2011.03514.x · Cited by 336
Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing
Yeom, K., Lee, Y., Han, J., Suh, M. R., & Kim, V. N. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl458 · Cited by 309
Silencing MicroRNA-155 Ameliorates Experimental Autoimmune Encephalomyelitis
Murugaiyan, G., Beynon, V., Mittal, A., Joller, N., & Weiner, H. L. (2011) · The Journal of Immunology · doi:10.4049/jimmunol.1003952 · Cited by 289
Ponomarev, E. D., Veremeyko, T., & Weiner, H. L. (2013) · Glia · doi:10.1002/glia.22363 · Cited by 284
Essential role of microRNA‐155 in the pathogenesis of autoimmune arthritis in mice
Blüml, S., Bonelli, M. M., Niederreiter, B., Puchner, A., Mayr, G., Hayer, S., Koenders, M. I., van den Berg, W. B., Smolen, J. S., & Redlich, K. (2011) · Arthritis & Rheumatism · doi:10.1002/art.30281 · Cited by 260
Micro‐RNA‐155 inhibits IFN‐γ signaling in CD4 + T cells
Banerjee, A., Schambach, F., DeJong, C. S., Hammond, S. M., & Reiner, S. L. (2009) · European Journal of Immunology · doi:10.1002/eji.200939381 · Cited by 252
Differential Micro RNA Expression in PBMC from Multiple Sclerosis Patients
Otaegui, D., Baranzini, S. E., Armañanzas, R., Calvo, B., Muñoz‐Culla, M., Khankhanian, P., Inza, I., Lozano, J. A., Castillo‐Triviño, T., Asensio, A., et al. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006309 · Cited by 250
Cox, M., Cairns, M. J., Gandhi, K. S., Carroll, A. P., Moscovis, S. M., Stewart, G. J., Broadley, S., Scott, R. J., Booth, D. R., & Lechner‐Scott, J. (2010) · PLoS ONE · doi:10.1371/journal.pone.0012132 · Cited by 239
Impaired neurosteroid synthesis in multiple sclerosis
Noorbakhsh, F., Ellestad, K. K., Maingat, F. G., Warren, K. G., Han, M. H., Steinman, L., Baker, G. B., & Power, C. (2011) · Brain · doi:10.1093/brain/awr200 · Cited by 239
Circulating microRNAs as candidate biomarkers in patients with systemic lupus erythematosus
Wang, H., Peng, W., Ouyang, X., Li, W., & Dai, Y. (2012) · Translational research · doi:10.1016/j.trsl.2012.04.002 · Cited by 229
Lindberg, R. L. P., Hoffmann, F., Mehling, M., Kühle, J., & Kappos, L. (2010) · European Journal of Immunology · doi:10.1002/eji.200940032 · Cited by 217
Altered miRNA expression in T regulatory cells in course of multiple sclerosis
Santis, G. D., Ferracin, M., Biondani, A., Caniatti, L. M., Tola, M. R., Castellazzi, M., Zagatti, B., Battistini, L., Borsellino, G., Fainardi, E., et al. (2010) · Journal of Neuroimmunology · doi:10.1016/j.jneuroim.2010.06.009 · Cited by 204
Waschbisch, A., Atiya, M., Linker, R. A., Potapov, S., Schwab, S., & Derfuss, T. J. (2011) · PLoS ONE · doi:10.1371/journal.pone.0024604 · Cited by 195
Micro-RNA dysregulation in multiple sclerosis favours pro-inflammatory T-cell-mediated autoimmunity
Guerau-de-Arellano, M., Smith, K. M., Godlewski, J., Liu, Y., Winger, R., Lawler, S. E., Whitacre, C. C., Racke, M. K., & Lovett-Racke, A. E. (2011) · Brain · doi:10.1093/brain/awr262 · Cited by 188
MicroRNA-155 Targets SMAD2 and Modulates the Response of Macrophages to Transforming Growth Factor-β
Louafi, F., Martinez-Nunez, R. T., & Sanchez-Elsner, T. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.146852 · Cited by 181
DSouza, S., Alinauskas, K. A., McCrea, E., Goodyer, C. G., & Antel, J. P. (1995) · Journal of Neuroscience · doi:10.1523/jneurosci.15-11-07293.1995 · Cited by 180
Self-citationFenoglio, C., Cantoni, C., Riz, M. A. D., Ridolfi, E., Cortini, F., Serpente, M., Villa, C., Comi, C., Monaco, F., Mellesi, L., et al. (2011) · Neuroscience Letters · doi:10.1016/j.neulet.2011.08.021 · Cited by 178
Circulating microRNAs involved in multiple sclerosis
Siegel, S. R., Mackenzie, J., Chaplin, G., Jablonski, N. G., & Griffiths, L. R. (2012) · Molecular Biology Reports · doi:10.1007/s11033-011-1441-7 · Cited by 177
B7/BB‐1 antigen expression on adult human microglia studied in vitro and in situ
Williams, K. C., Ulvestad, E., & Antel, J. P. (1994) · European Journal of Immunology · doi:10.1002/eji.1830241217 · Cited by 169
Self-citationLusardi, T. A., Farr, C. D., Faulkner, C. L., Pignataro, G., Yang, T., Lan, J., Simon, R. P., & Saugstad, J. A. (2009) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.2009.253 · Cited by 158
Tarassishin, L., Loudig, O. D., Bauman, A., Shafit‐Zagardo, B., Suh, H.-S., & Lee, S. C. (2011) · Glia · doi:10.1002/glia.21233 · Cited by 130
Role of microRNA-155 in autoimmunity
Leng, R.-X., Pan, H.-F., Qin, W.-Z., Chen, G.-M., & Ye, D.-Q. (2011) · Cytokine & Growth Factor Reviews · doi:10.1016/j.cytogfr.2011.05.002 · Cited by 118
Altered microRNA expression in B lymphocytes in multiple sclerosis
Sievers, C., Meira, M., Hoffmann, F., da Fontoura, P. P., Kappos, L., & Lindberg, R. L. P. (2012) · Clinical Immunology · doi:10.1016/j.clim.2012.04.002 · Cited by 118
Monocytes in multiple sclerosis: phenotype and cytokine profile
Kouwenhoven, M. C. M., Teleshova, N., Özenci, V., Press, R., & Link, H. (2001) · Journal of Neuroimmunology · doi:10.1016/s0165-5728(00)00396-9 · Cited by 109
MicroRNAs in rheumatoid arthritis
Ceribelli, A., Nahid, M. A., Satoh, M., & Chan, E. K. L. (2011) · FEBS Letters · doi:10.1016/j.febslet.2011.05.020 · Cited by 108
Boneschi, F. M., Fenoglio, C., Brambilla, P., Sorosina, M., Giacalone, G., Esposito, F., Serpente, M., Cantoni, C., Ridolfi, E., Rodegher, M., et al. (2011) · Neuroscience Letters · doi:10.1016/j.neulet.2011.11.006 · Cited by 93
Li, J., Smyth, P. C., Cahill, S., Denning, K. M., Flavin, R., Aherne, S. T., Pirotta, M., Guenther, S. M., O’Leary, J. J., & Sheils, O. M. (2008) · BMC Biotechnology · doi:10.1186/1472-6750-8-10 · Cited by 91
miRNA profiling for biomarker discovery in multiple sclerosis: From microarray to deep sequencing
Guerau‐de‐Arellano, M., Alder, H., Özer, H. G., Lovett‐Racke, A. E., & Racke, M. K. (2011) · Journal of Neuroimmunology · doi:10.1016/j.jneuroim.2011.10.006 · Cited by 85
Major Differences in the Responses of Primary Human Leukocyte Subsets to IFN-β
van Boxel-Dezaire, A. H. H., Zula, J. A., Xu, Y., Ransohoff, R. M., Jacobberger, J. W., & Stark, G. R. (2010) · The Journal of Immunology · doi:10.4049/jimmunol.0902314 · Cited by 71
Filion, L. G., Matusevičius, D., Graziani-Bowering, G. M., Kumar, A., & Freedman, M. S. (2003) · Clinical Immunology · doi:10.1016/s1521-6616(02)00028-1 · Cited by 63
miR-15a and 16-1 Are Downregulated in CD4+T Cells of Multiple Sclerosis Relapsing Patients
Lorenzi, J. C. C., Brum, D. G., Zanette, D. L., Souza, A. D. P. A., Barbuzano, F. G., dos Santos, A. C., Barreira, A. A., & Silva, W. A. (2012) · International Journal of Neuroscience · doi:10.3109/00207454.2012.678444 · Cited by 59
Lescher, J., Paap, F., Schultz, V., Redenbach, L., Scheidt, U., Rosewich, H., Nessler, S., Fuchs, E., Gärtner, J., Brück, W., et al. (2012) · Journal of Neuroimmunology · doi:10.1016/j.jneuroim.2012.02.012 · Cited by 53
Dendritic Cell Differentiation Signals Induce Anti-Inflammatory Properties in Human Adult Microglia
Lambert, C., Desbarats, J., Arbour, N., Hall, J. A., Olivier, A., Bar-Or, A., & Antel, J. P. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.181.12.8288 · Cited by 50
Self-citationDistinct migratory and cytokine responses of human microglia and macrophages to ATP
Lambert, C., Ase, A. R., Séguéla, P., & Antel, J. P. (2010) · Brain Behavior and Immunity · doi:10.1016/j.bbi.2010.02.010 · Cited by 43
Self-citationTh1 Polarization of CD4+ T Cells by Toll-Like Receptor 3-Activated Human Microglia
Jack, C. S., Arbour, N., Blain, M., Meier, U., Prat, A., & Antel, J. P. (2007) · Journal of Neuropathology & Experimental Neurology · doi:10.1097/nen.0b013e3181492a7 · Cited by 36
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Filippi, M., Bar-Or, A., Piehl, F., Preziosa, P., Solari, A., Vukusic, S., & Rocca, M. A. (2018) · Nature Reviews Disease Primers · doi:10.1038/s41572-018-0041-4 · Cited by 1,562
Self-citationMicroglia and brain macrophages in the molecular age: from origin to neuropsychiatric disease
Prinz, M., & Priller, J. (2014) · Nature Reviews Neuroscience · doi:10.1038/nrn3722 · Cited by 1,329
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Essandoh, K., Li, Y., Huo, J., & Fan, G. (2016) · Shock · doi:10.1097/shk.0000000000000604 · Cited by 610
“Polarized macrophages interact with various tissues and organs in diseases such as obesity (81–86), cancer (62–65), and multiple sclerosis (87, 88), thus affecting pathophysiology of such disorders.”
Background
Metadata
- Authors
- Moore, C. S., Rao, V. T. S., Durafourt, B. A., Bedell, B. J., Ludwin, S. K., Bar‐Or, A., & Antel, J. P.
- Affiliations
- Montreal Neurological Institute and Hospital (CA)
- Queen's University (CA)
- Corresponding author
- Moore, Craig S.
- Venue
- Annals of Neurology
- Publisher
- Wiley
- Published
- 2013-09-23
- Volume
- 74
- Issue
- 5
- Pages
- 709-720
- ISSN
- 0364-5134 (ISSN-L)
- 1531-8249
- OpenAlex
- W1915908724
- Cited by
- 219
- References
- 48
- Citations by year
- 2026: 8 · 2025: 6 · 2024: 13 · 2023: 11 · 2022: 13 · 2021: 15 · 2020: 25 · 2019: 26 · 2018: 23 · 2017: 24 · 2016: 23 · 2015: 15 · 2014: 15 · 2013: 2
- FWCI
- 13.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Annals of Neurology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Clinical Neurology › Multiple Sclerosis Research Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- miR-155, multiple sclerosis, myeloid cell polarization, microglia, blood-derived macrophages, CD14+ monocytes, M1 macrophage polarization, inflammatory cytokines, costimulatory molecules, T cell response, relapsing-remitting multiple sclerosis, neuroinflammation
- MeSH terms
- Adult, Aged, Brain, Female, Humans, Macrophages, Male, Middle Aged, Multiple Sclerosis, T-Lymphocytes, Cell Polarity, Microglia, Myeloid Cells, MicroRNAs, Cell Proliferation, Adaptive Immunity
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