Maximizing the Potency of siRNA Lipid Nanoparticles for Hepatic Gene Silencing In Vivo**

Knocking down barriers: advances in siRNA delivery

Whitehead, K. A., Langer, R., & Anderson, D. G. (2009) · Nature Reviews Drug Discovery · doi:10.1038/nrd2742 · Cited by 3,115 · see citing works

Rational design of cationic lipids for siRNA delivery

Semple, S. C., Akinc, A., Chen, J., Sandhu, A. P., Mui, B. L., Cho, C. K., Sah, D. W. Y., Stebbing, D., Crosley, E. J., Yaworski, E., et al. (2010) · Nature Biotechnology · doi:10.1038/nbt.1602 · Cited by 1,822 · see citing works

RNAi-mediated gene silencing in non-human primates

Zimmermann, T. S., Lee, A. C. H., Akinc, A., Bramlage, B., Bumcrot, D., Fedoruk, M. N., Harborth, J., Heyes, J. A., Jeffs, L. B., John, M., et al. (2006) · Nature · doi:10.1038/nature04688 · Cited by 1,357 · see citing works

Targeted delivery of RNAi therapeutics with endogenous and exogenous ligand-based mechanisms

Akinc, A., Querbes, W., De, S., Qin, J., Frank-Kamenetsky, M., Jayaprakash, K. N., Jayaraman, M., Rajeev, K. G., Cantley, W. L., Dorkin, J. R., et al. (2010) · Molecular Therapy · doi:10.1038/mt.2010.85 · Cited by 1,261 · see citing works

Interfering with disease: a progress report on siRNA-based therapeutics

de Fougerolles, A., Vornlocher, H., Maraganore, J. M., & Lieberman, J. (2007) · Nature Reviews Drug Discovery · doi:10.1038/nrd2310 · Cited by 1,202 · see citing works

On the mechanism whereby cationic lipids promote intracellular delivery of polynucleic acids

Hafez, I., Maurer, N., & Cullis, P. R. (2001) · Gene Therapy · doi:10.1038/sj.gt.3301506 · Cited by 601 · see citing works

Cationic lipid saturation influences intracellular delivery of encapsulated nucleic acids

Heyes, J., Palmer, L., Bremner, K., & MacLachlan, I. (2005) · Journal of Controlled Release · doi:10.1016/j.jconrel.2005.06.014 · Cited by 572 · see citing works

Nonviral delivery of synthetic siRNAs in vivo

Akhtar, S., & Benter, I. F. (2007) · Journal of Clinical Investigation · doi:10.1172/jci33494 · Cited by 464 · see citing works

Development of Lipidoid–siRNA Formulations for Systemic Delivery to the Liver

Akinc, A., Goldberg, M. S., Qin, J., Dorkin, J. R., Gamba‐Vitalo, C., Maier, M. A., Jayaprakash, K. N., Jayaraman, M., Rajeev, K. G., Manoharan, M., et al. (2009) · Molecular Therapy · doi:10.1038/mt.2009.36 · Cited by 353 · see citing works

Modulation of Membrane Fusion by Asymmetric Transbilayer Distributions of Amino Lipids

Bailey, A. L., & Cullis, P. R. (1994) · Biochemistry · doi:10.1021/bi00208a007 · Cited by 143 · see citing works

The role of apolipoprotein E in the elimination of liposomes from blood by hepatocytes in the mouse

Yan, X., Kuipers, F., Havekes, L. M., Havinga, R., Dontje, B., Poelstra, K., Scherphof, G. L., & Kamps, J. A. A. M. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2004.12.137 · Cited by 133 · see citing works

Ionization Behavior of Amino Lipids for siRNA Delivery: Determination of Ionization Constants, SAR, and the Impact of Lipid pKa on Cationic Lipid−Biomembrane Interactions

Zhang, J., Fan, H., Levorse, D. A., & Crocker, L. S. (2011) · Langmuir · doi:10.1021/la104590k · Cited by 95 · see citing works

Transbilayer transport of phosphatidic acid in response to transmembrane pH gradients

Eastman, S. J., Hope, M. J., & Cullis, P. R. (1991) · Biochemistry · doi:10.1021/bi00221a002 · Cited by 86 · see citing works

Selective silencing of a mutant transthyretin allele by small interfering RNAs

Kurosawa, T., Igarashi, S., Nishizawa, M., & Onodera, O. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.09.142 · Cited by 43 · see citing works

Cationic Liposomes as an Oligonucleotide Carrier: Mechanism of Action

Zelphati, O., & Szoka, F. C. (1997) · Journal of Liposome Research · doi:10.3109/08982109709035480 · Cited by 26 · see citing works

Lipid nanoparticles for mRNA delivery

Hou, X., Zaks, T., Langer, R., & Dong, Y. (2021) · Nature Reviews Materials · doi:10.1038/s41578-021-00358-0 · Cited by 4,076 · see citing works

Advances in COVID-19 mRNA vaccine development

Fang, E., Liu, X., Li, M., Zhang, Z., Song, L., Zhu, B., Wu, X., Liu, J., Zhao, D., & Li, Y. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-00950-y · Cited by 601 · see citing works

Lipid Nanoparticles for Drug Delivery

Xu, L., Wang, X., Liu, Y., Yang, G., Falconer, R. J., & Zhao, C. (2021) · Advanced NanoBiomed Research · doi:10.1002/anbr.202100109 · Cited by 568 · see citing works

Non‐Viral CRISPR/Cas Gene Editing In Vitro and In Vivo Enabled by Synthetic Nanoparticle Co‐Delivery of Cas9 mRNA and sgRNA

Miller, J. B., Zhang, S., Kos, P., Xiong, H., Zhou, K., Perelman, S. S., Zhu, H., & Siegwart, D. J. (2017) · Angewandte Chemie International Edition · doi:10.1002/anie.201610209 · Cited by 540 · see citing works

The landscape of mRNA nanomedicine

Huang, X., Kong, N., Zhang, X., Cao, Y., Langer, R., & Tao, W. (2022) · Nature Medicine · doi:10.1038/s41591-022-02061-1 · Cited by 511 · see citing works

Nanotechnologies in delivery of mRNA therapeutics using nonviral vector-based delivery systems

Guan, S., & Rosenecker, J. (2017) · Gene Therapy · doi:10.1038/gt.2017.5 · Cited by 389 · see citing works

Next‐Generation Vaccines: Nanoparticle‐Mediated DNA and mRNA Delivery

Ho, W., Gao, M., Li, F., Li, Z., Zhang, X., & Xu, X. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202001812 · Cited by 261 · see citing works

Structure and Function of Cationic and Ionizable Lipids for Nucleic Acid Delivery

Sun, D., & Lu, Z. (2023) · Pharmaceutical Research · doi:10.1007/s11095-022-03460-2 · Cited by 253 · see citing works

Adjuvant lipidoid-substituted lipid nanoparticles augment the immunogenicity of SARS-CoV-2 mRNA vaccines

Han, X., Alameh, M., Butowska, K., Knox, J. J., Lundgreen, K. A., Ghattas, M., Gong, N., Xue, L., Xu, Y., Lavertu, M., et al. (2023) · Nature Nanotechnology · doi:10.1038/s41565-023-01404-4 · Cited by 248 · see citing works

Lipid-Based Nanocarriers for RNA Delivery

Xue, H. Y., Guo, P., Wen, W., & Wong, H. (2015) · Current Pharmaceutical Design · doi:10.2174/1381612821666150531164540 · Cited by 202 · see citing works

Lipid nanoparticle-mediated drug delivery to the brain

Khare, P., Edgecomb, S. X., Hamadani, C. M., Tanner, E. E. L., & S Manickam, D. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.114861 · Cited by 187 · see citing works

Hepatocyte‐Specific Delivery of siRNAs Conjugated to Novel Non‐nucleosidic Trivalent N ‐Acetylgalactosamine Elicits Robust Gene Silencing in Vivo

Rajeev, K. G., Nair, J. K., Jayaraman, M., Charissé, K., Taneja, N., O′Shea, J., Willoughby, J. L. S., Yucius, K., Nguyen, T. M., Shulga‐Morskaya, S., et al. (2015) · ChemBioChem · doi:10.1002/cbic.201500023 · Cited by 185 · see citing works

RNA Nanotechnology-Mediated Cancer Immunotherapy

Lin, Y., Wang, Y., Blake, S., Yu, M., Mei, L., Wang, H., & Shi, J. (2019) · Theranostics · doi:10.7150/thno.35568 · Cited by 173 · see citing works

Ionizable Lipid Nanoparticles for <i>In Vivo</i> mRNA Delivery to the Placenta during Pregnancy

Swingle, K. L., Safford, H. C., Geisler, H. C., Hamilton, A. G., Thatte, A. S., Billingsley, M. M., Joseph, R. A., Mrksich, K., Padilla, M. S., Ghalsasi, A. A., et al. (2023) · Journal of the American Chemical Society · doi:10.1021/jacs.2c12893 · Cited by 145 · see citing works

A Systematic Study of Unsaturation in Lipid Nanoparticles Leads to Improved mRNA Transfection In Vivo

Lee, S. M., Cheng, Q., Yu, X., Liu, S., Johnson, L. T., & Siegwart, D. J. (2020) · Angewandte Chemie International Edition · doi:10.1002/anie.202013927 · Cited by 140 · see citing works

Placenta-tropic VEGF mRNA lipid nanoparticles ameliorate murine pre-eclampsia

Swingle, K. L., Hamilton, A. G., Safford, H. C., Geisler, H. C., Thatte, A. S., Palanki, R., Murray, A. M., Han, E. L., Mukalel, A. J., Han, X., et al. (2025) · Nature · doi:10.1038/s41586-024-08291-2 · Cited by 129 · see citing works

Iterative Design of Ionizable Lipids for Intramuscular mRNA Delivery

Tilstra, G., Couture‐Senécal, J., Lau, Y. M. A., Manning, A. M., Wong, D., Janaeska, W. W., Wuraola, T. A., Pang, J., & Khan, O. F. (2023) · Journal of the American Chemical Society · doi:10.1021/jacs.2c10670 · Cited by 129 · see citing works

Clinical delivery of circular RNA: Lessons learned from RNA drug development

Young, T. L., Wang, K. C., Varley, A., & Li, B. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.114826 · Cited by 126 · see citing works

Development of multivalent mRNA vaccine candidates for seasonal or pandemic influenza

Chivukula, S., Plitnik, T., Tibbitts, T., Karve, S., Dias, A., Zhang, D., Goldman, R., Gopani, H., Khanmohammed, A., Sarode, A., et al. (2021) · npj Vaccines · doi:10.1038/s41541-021-00420-6 · Cited by 117 · see citing works

Delivery of modified mRNA to damaged myocardium by systemic administration of lipid nanoparticles

Evers, M. J. W., Du, W., Yang, Q., Kooijmans, S. A. A., Vink, A., van Steenbergen, M., Vader, P., de Jager, S. C. A., Fuchs, S. A., Mastrobattista, E., et al. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2022.01.027 · Cited by 113 · see citing works

Ionizable Lipid Nanoparticles with Integrated Immune Checkpoint Inhibition for mRNA CAR T Cell Engineering

Hamilton, A. G., Swingle, K. L., Joseph, R. A., Mai, D., Gong, N., Billingsley, M. M., Alameh, M., Weissman, D., Sheppard, N. C., June, C. H., et al. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202301515 · Cited by 108 · see citing works

A single methylene group in oligoalkylamine‐based cationic polymers and lipids promotes enhanced mRNA delivery

Jarzębińska, A., Pasewald, T., Lambrecht, J., Mykhaylyk, O., Kümmerling, L., Beck, P., Hasenpusch, G., Rudolph, C., Plank, C., & Dohmen, C. (2016) · Angewandte Chemie International Edition · doi:10.1002/anie.201603648 · Cited by 101 · see citing works

Ionizable Amphiphilic Dendrimer‐Based Nanomaterials with Alkyl‐Chain‐Substituted Amines for Tunable siRNA Delivery to the Liver Endothelium In Vivo

Khan, O. F., Zaia, E. W., Yin, H., Bogorad, R. L., Pelet, J. M., Webber, M. J., Zhuang, I., Dahlman, J. E., Langer, R., & Anderson, D. G. (2014) · Angewandte Chemie International Edition · doi:10.1002/anie.201408221 · Cited by 101 · see citing works

Analysis of PEG-lipid anchor length on lipid nanoparticle pharmacokinetics and activity in a mouse model of traumatic brain injury

Waggoner, L. E., Miyasaki, K. F., & Kwon, E. J. (2023) · Biomaterials Science · doi:10.1039/d2bm01846b · Cited by 98 · see citing works

Modification of Lipid-Based Nanoparticles: An Efficient Delivery System for Nucleic Acid-Based Immunotherapy

Zhang, C., Ma, Y., Zhang, J., Kuo, J. C., Zhang, Z., Xie, H., Zhu, J., & Liu, T. (2022) · Molecules · doi:10.3390/molecules27061943 · Cited by 93 · see citing works

Supramolecular Self‐Assembled Nanoparticles Mediate Oral Delivery of Therapeutic TNF‐α siRNA against Systemic Inflammation

Yin, L., Song, Z., Qu, Q., Kim, K. H., Zheng, N., Yao, C., Chaudhury, I., Tang, H., Gabrielson, N. P., Uckun, F. M., et al. (2013) · Angewandte Chemie International Edition · doi:10.1002/anie.201209991 · Cited by 92 · see citing works

The siRNAsome: A Cation‐Free and Versatile Nanostructure for siRNA and Drug Co‐delivery

Zheng, M., Jiang, T., Yang, W., Zou, Y., Wu, H., Liu, X., Zhu, F., Qian, R., Ling, D., McDonald, K. L., et al. (2019) · Angewandte Chemie International Edition · doi:10.1002/anie.201814289 · Cited by 88 · see citing works

siRNA Functionalized Lipid Nanoparticles (LNPs) in Management of Diseases

Kalita, T., Dezfouli, S. A., Pandey, L. M., & Uludağ, H. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14112520 · Cited by 87 · see citing works

Rational design and combinatorial chemistry of ionizable lipids for RNA delivery

Xu, Y., Golubovic, A., Xu, S., Pan, A., & Li, B. (2023) · Journal of Materials Chemistry B · doi:10.1039/d3tb00649b · Cited by 84 · see citing works

Synthetic Approaches for Nucleic Acid Delivery: Choosing the Right Carriers

Ni, R., Feng, R., & Chau, Y. (2019) · Life · doi:10.3390/life9030059 · Cited by 80 · see citing works

siRNA therapeutics and its challenges: Recent advances in effective delivery for cancer therapy

Paul, A., Muralidharan, A., Biswas, A., Kamath, B. V., Joseph, A., & Alex, A. T. (2022) · OpenNano · doi:10.1016/j.onano.2022.100063 · Cited by 77 · see citing works

Enzyme-Catalyzed One-Step Synthesis of Ionizable Cationic Lipids for Lipid Nanoparticle-Based mRNA COVID-19 Vaccines

Li, Z., Zhang, X., Ho, W., Li, F., Gao, M., Bai, X., & Xu, X. (2022) · ACS Nano · doi:10.1021/acsnano.2c07822 · Cited by 75 · see citing works

Non‐Viral CRISPR/Cas Gene Editing In Vitro and In Vivo Enabled by Synthetic Nanoparticle Co‐Delivery of Cas9 mRNA and sgRNA

Miller, J. B., Zhang, S., Kós, P., Xiong, H., Zhou, K., Perelman, S. S., Zhu, H., & Siegwart, D. J. (2016) · Angewandte Chemie · doi:10.1002/ange.201610209 · Cited by 74 · see citing works

Nonspherical Metal‐Based Nanoarchitectures: Synthesis and Impact of Size, Shape, and Composition on Their Biological Activity

Zare‬, E. N., Zheng, X.-Q., Makvandi, P., Gheybi, H., Sartorius, R., Yiu, C. K. Y., Adeli, M., Wu, A., Zarrabi, A., Varma, R. S., et al. (2021) · Small · doi:10.1002/smll.202007073 · Cited by 74 · see citing works

Real‐Time pH‐Dependent Self‐Assembly of Ionisable Lipids from COVID‐19 Vaccines and In Situ Nucleic Acid Complexation

Yu, H., Angelova, A., Angelov, B., Dyett, B., Matthews, L., Zhang, Y., Mohamad, M. E., Cai, X., Valimehr, S., Drummond, C. J., et al. (2023) · Angewandte Chemie International Edition · doi:10.1002/anie.202304977 · Cited by 70 · see citing works

Mixing lipids to manipulate the ionization status of lipid nanoparticles for specific tissue targeting

Shobaki, N., Sato, Y., & Harashima, H. (2018) · International Journal of Nanomedicine · doi:10.2147/ijn.s188016 · Cited by 65 · see citing works

Amniotic fluid stabilized lipid nanoparticles for in utero intra-amniotic mRNA delivery

Swingle, K. L., Billingsley, M. M., Bose, S. K., White, B., Palanki, R., Dave, A., Patel, S. K., Gong, N., Hamilton, A. G., Alameh, M.-G., et al. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2021.10.031 · Cited by 64 · see citing works

Nucleic Acid-Loaded Lipid Nanoparticle Interactions with Model Endosomal Membranes

Spadea, A., Jackman, M. J., Cui, L., Pereira, S. G. T., Lawrence, M. J., Campbell, R. A., & Ashford, M. B. (2022) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.2c06065 · Cited by 62 · see citing works

Advances and prospects of RNA delivery nanoplatforms for cancer therapy

Attia, M. S., Kijanka, G. S., Nguyen, N., Zhang, J., & An, H. (2024) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2024.09.009 · Cited by 64 · see citing works

Simultaneous, Single-Particle Measurements of Size and Loading Give Insights into the Structure of Drug-Delivery Nanoparticles

Kamanzi, A., Gu, Y., Tahvildari, R., Friedenberger, Z., Zhu, X., Berti, R., Kurylowicz, M., Witzigmann, D., Kulkarni, J. A., Leung, J., et al. (2021) · ACS Nano · doi:10.1021/acsnano.1c04862 · Cited by 58 · see citing works

Lipid‐Polymer Hybrid “Particle‐in‐Particle” Nanostructure Gene Delivery Platform Explored for Lyophilizable DNA and mRNA COVID‐19 Vaccines

Li, Z., Zhang, X., Ho, W., Bai, X., Jaijyan, D. K., Li, F., Kumar, R., Kolloli, A., Subbian, S., Zhu, H., et al. (2022) · Advanced Functional Materials · doi:10.1002/adfm.202204462 · Cited by 59 · see citing works

Therapeutic RNA-silencing oligonucleotides in metabolic diseases

Goga, A., & Stoffel, M. (2022) · Nature Reviews Drug Discovery · doi:10.1038/s41573-022-00407-5 · Cited by 58 · see citing works

Role of PCIF1‐mediated 5′‐cap N6‐methyladeonsine mRNA methylation in colorectal cancer and anti‐PD‐1 immunotherapy

Wang, L., Wu, L., Zhu, Z., Zhang, Q., Li, W., Gonzalez, G. M., Wang, Y., & Rana, T. M. (2022) · The EMBO Journal · doi:10.15252/embj.2022111673 · Cited by 58 · see citing works

Targeting materials and strategies for RNA delivery

Lin, L., Su, K., Cheng, Q., & Liu, S. (2023) · Theranostics · doi:10.7150/thno.87316 · Cited by 61 · see citing works

Application of lipid-based nanoparticles in cancer immunotherapy

Zhang, Z., Yao, S., Hu, Y., Zhao, X. B., & Lee, R. J. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.967505 · Cited by 57 · see citing works

Delivering the CRISPR/Cas9 system for engineering gene therapies: Recent cargo and delivery approaches for clinical translation

Foley, R. A., Sims, R. A., Duggan, E. C., Olmedo, J. K., Ma, R., & Jonas, S. J. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.973326 · Cited by 57 · see citing works

Lipid based nanocarriers for effective drug delivery and treatment of diabetes associated liver fibrosis

Salunkhe, S. A., Chitkara, D., Mahato, R. I., & Mittal, A. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.04.003 · Cited by 54 · see citing works

Endolysosomal trapping of therapeutics and endosomal escape strategies

Ahmad, A., Khan, J. M., Paray, B. A., Rashid, K., & Parvez, A. (2024) · Drug Discovery Today · doi:10.1016/j.drudis.2024.104070 · Cited by 55 · see citing works

Inverse Cubic and Hexagonal Mesophase Evolution within Ionizable Lipid Nanoparticles Correlates with mRNA Transfection in Macrophages

Yu, H., Iscaro, J., Dyett, B., Zhang, Y., Seibt, S., Martínez, N. P., White, J., Drummond, C. J., Bozinovski, S., & Zhai, J. (2023) · Journal of the American Chemical Society · doi:10.1021/jacs.3c08729 · Cited by 52 · see citing works

Polysarcosine-Functionalized mRNA Lipid Nanoparticles Tailored for Immunotherapy

Wilhelmy, C., Keil, I. S., Uebbing, L., Schroer, M. A., Franke, D., Nawroth, T., Barz, M., Şahin, U., Haas, H., Diken, M., et al. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15082068 · Cited by 51 · see citing works

Lipid nanoparticles for RNA delivery: Self-assembling vs driven-assembling strategies

Nele, V., Campani, V., Moosavian, S. A., & Rosa, G. D. (2024) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2024.115291 · Cited by 53 · see citing works

Role of drug delivery technologies in the success of COVID-19 vaccines: a perspective

Labouta, H. I., Langer, R., Cullis, P. R., Merkel, O. M., Prausnitz, M. R., Gomaa, Y. A., Nogueira, S. S., & Kumeria, T. (2022) · Drug Delivery and Translational Research · doi:10.1007/s13346-022-01146-1 · Cited by 51 · see citing works

Analytical techniques for the characterization of nanoparticles for mRNA delivery

Nogueira, S. S., Samaridou, E., Simon, J., Simon, F., Beck‐Broichsitter, M., & Mehta, A. (2024) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2024.114235 · Cited by 51 · see citing works

Cellular Trafficking of Nanotechnology‐Mediated mRNA Delivery

Huang, P., Deng, H., Wang, C., Zhou, Y., & Chen, X. S. (2023) · Advanced Materials · doi:10.1002/adma.202307822 · Cited by 49 · see citing works

Ionizable Amino Lipids Distribution and Effects on DSPC/Cholesterol Membranes: Implications for Lipid Nanoparticle Structure

Dehghani‐Ghahnaviyeh, S., Smith, M., Xia, Y., Dousis, A. D., Grossfield, A. M., & Sur, S. (2023) · The Journal of Physical Chemistry B · doi:10.1021/acs.jpcb.3c01296 · Cited by 46 · see citing works

Lipid nanoparticle (LNP) mediated mRNA delivery in neurodegenerative diseases

Moosavi, S. G., Rahiman, N., Jaafari, M. R., & Arabi, L. (2025) · Journal of Controlled Release · doi:10.1016/j.jconrel.2025.113641 · Cited by 52 · see citing works

Engineered multi-domain lipid nanoparticles for targeted delivery

Liu, Z., Chen, J., Xu, M., Ho, S., Wei, Y., Ho, H., & Yong, K. (2025) · Chemical Society Reviews · doi:10.1039/d4cs00891j · Cited by 60 · see citing works

Lipid nanoparticles as the drug carrier for targeted therapy of hepatic disorders

Chu, R., Wang, Y., Kong, J., Pan, T. S., Yang, Y., & He, J. F. (2024) · Journal of Materials Chemistry B · doi:10.1039/d3tb02766j · Cited by 48 · see citing works

Computational Insights into the Role of Cholesterol in Inverted Hexagonal Phase Stabilization and Endosomal Drug Release

Ramezanpour, M., & Tieleman, D. P. (2022) · Langmuir · doi:10.1021/acs.langmuir.2c00430 · Cited by 42 · see citing works

Nanotechnology strategies to address challenges in topical and cellular delivery of siRNAs in skin disease therapy

Silvestrini, A. V. P., Morais, M. F., Debiasi, B. W., Praça, F. S. G., & Bentley, M. V. L. B. (2024) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2024.115198 · Cited by 40 · see citing works

In Vivo Endothelial Cell Gene Silencing by siRNA‐LNPs Tuned with Lipoamino Bundle Chemical and Ligand Targeting

Yazdi, M., Pöhmerer, J., Kafshgari, M. H., Seidl, J., Grau, M., Höhn, M., Vetter, V., Hoch, C. C., Wollenberg, B., Multhoff, G., et al. (2024) · Small · doi:10.1002/smll.202400643 · Cited by 34 · see citing works

Enhancing siRNA cancer therapy: Multifaceted strategies with lipid and polymer-based carrier systems

Dastgerdi, N. K., Dastgerdi, N. K., Bayraktutan, H., Costabile, G., Atyabi, F., Dinarvand, R., Longobardi, G., Alexander, C., & Conte, C. (2024) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2024.124545 · Cited by 39 · see citing works

Emerging prospects of mRNA cancer vaccines: mechanisms, formulations, and challenges in cancer immunotherapy

Laila, U. E., An, W., & Xu, Z.-X. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1448489 · Cited by 34 · see citing works

A Systematic Study of Unsaturation in Lipid Nanoparticles Leads to Improved mRNA Transfection In Vivo

Lee, S. M., Cheng, Q., Yu, X., Liu, S., Johnson, L. T., & Siegwart, D. J. (2020) · Angewandte Chemie · doi:10.1002/ange.202013927 · Cited by 32 · see citing works

Lipid nanoparticles: a promising tool for nucleic acid delivery in cancer immunotherapy

Abaza, T., Mohamed, E., & Zaky, M. Y. (2025) · Medical Oncology · doi:10.1007/s12032-025-02939-3 · Cited by 33 · see citing works

Strategies to load therapeutics into polysaccharide-based nanogels with a focus on microfluidics: A review

Zoratto, N., Montanari, E., Viola, M., Wang, J., Coviello, T., Meo, C. D., & Matricardi, P. (2021) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2021.118119 · Cited by 30 · see citing works

Recent Progress of Small Interfering RNA Delivery on the Market and Clinical Stage

Guo, F., Li, Y., Yu, W., Fu, Y., Zhang, J., & Cao, H. (2024) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.3c01158 · Cited by 29 · see citing works

Harnessing Topology and Stereochemistry of Glycidylamine‐Derived Lipid Nanoparticles for in Vivo mRNA Delivery to Immune Cells in Spleen and Their Application for Cancer Vaccination

Elwakil, M. M. A., Suzuki, R., Khalifa, A. M., Elshami, R. M., Isono, T., Elewa, Y. H. A., Sato, Y., Nakamura, T., Satoh, T., & Harashima, H. (2023) · Advanced Functional Materials · doi:10.1002/adfm.202303795 · Cited by 28 · see citing works

pH‐Dependent Lyotropic Liquid Crystalline Mesophase and Ionization Behavior of Phytantriol‐Based Ionizable Lipid Nanoparticles

Yu, H., Dyett, B., Kirby, N. M., Cai, X., Mohamad, M. E., Bozinovski, S., Drummond, C. J., & Zhai, J. (2024) · Small · doi:10.1002/smll.202309200 · Cited by 29 · see citing works

mRNA cancer vaccines from bench to bedside: a new era in cancer immunotherapy

Shariati, A., Khani, P., Nasri, F., Afkhami, H., Khezrpour, A., Kamrani, S., Shariati, F. S., Alavimanesh, S., & Modarressi, M. H. (2024) · Biomarker Research · doi:10.1186/s40364-024-00692-9 · Cited by 27 · see citing works

Real‐Time pH‐Dependent Self‐Assembly of Ionisable Lipids from COVID‐19 Vaccines and In Situ Nucleic Acid Complexation

Yu, H., Angelova, A., Angelov, B., Dyett, B., Matthews, L., Zhang, Y., Mohamad, M. E., Cai, X., Valimehr, S., Drummond, C. J., et al. (2023) · Angewandte Chemie · doi:10.1002/ange.202304977 · Cited by 26 · see citing works

Development of a Combined Lipid-Based Nanoparticle Formulation for Enhanced siRNA Delivery to Vascular Endothelial Cells

He, Y., Bi, D., Plantinga, J. A., Molema, G., Bussmann, J., & Kamps, J. A. A. M. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14102086 · Cited by 23 · see citing works

Recent Advances in Lipid Nanoparticles: Nucleic Acid Therapeutics and Targeting Strategies

Sarmah, S., Baidya, S., & De, M. (2025) · Small · doi:10.1002/smll.202506812 · Cited by 25 · see citing works

Methodical Design of Viral Vaccines Based on Avant-Garde Nanocarriers: A Multi-Domain Narrative Review

Raoufi, E., Bahramimeimandi, B., Salehi‐Shadkami, M., Chaosri, P., & Mozafari, M. R. (2021) · Biomedicines · doi:10.3390/biomedicines9050520 · Cited by 21 · see citing works

The siRNAsome: A Cation‐Free and Versatile Nanostructure for siRNA and Drug Co‐delivery

Zheng, M., Jiang, T., Yang, W., Zou, Y., Wu, H., Liu, X., Zhu, F., Qian, R., Ling, D., McDonald, K. L., et al. (2019) · Angewandte Chemie · doi:10.1002/ange.201814289 · Cited by 21 · see citing works

Optimization of HPLC CAD method for simultaneous analysis of different lipids in lipid nanoparticles with analytical QbD

Kim, K., Lee, J., Lee, J. C., Maharjan, R., Oh, H., Lee, K., Kim, N. A., & Jeong, S. H. (2023) · Journal of Chromatography A · doi:10.1016/j.chroma.2023.464375 · Cited by 21 · see citing works

Combining molecular dynamics simulations and x-ray scattering techniques for the accurate treatment of protonation degree and packing of ionizable lipids in monolayers

Grava, M., Ibrahim, M. A., Sudarsan, A., Pusterla, J. M., Philipp, J., Rädler, J. O., Schwierz, N., & Schneck, E. (2023) · The Journal of Chemical Physics · doi:10.1063/5.0172552 · Cited by 21 · see citing works

Nonviral targeted mRNA delivery: principles, progresses, and challenges

He, X., Li, G., Huang, L., Shi, H., Zhong, S., Zhao, S., Jiao, X., Xin, J., Yin, X., Liu, S., et al. (2025) · MedComm · doi:10.1002/mco2.70035 · Cited by 20 · see citing works

Lipid‐Based Nanocarriers as Drug Delivery System and Its Applications

Jain, V. K., Kumar, H., Chand, P., Jain, S., & P, P. S. (2021) · doi:10.1002/9781119711698.ch1 · Cited by 20 · see citing works

Structure–activity relationships of pH-responsive and ionizable lipids for gene delivery

Zou, Y., Zhou, Q., Zhao, Y., Zhi, D., Chen, H., Wang, R., Ju, B., & Zhang, S. (2022) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2022.121596 · Cited by 19 · see citing works

Ionic liquid-coated lipid nanoparticles increase siRNA uptake into CNS targets

Khare, P., Edgecomb, S. X., Hamadani, C. M., Conway, J. F., Tanner, E. E. L., & Manickam, D. S. (2023) · Nanoscale Advances · doi:10.1039/d3na00699a · Cited by 19 · see citing works

Genetic and functional correction of argininosuccinate lyase deficiency using CRISPR adenine base editors

Jalil, S., Keskinen, T., Juutila, J., Sartori-Maldonado, R., Euro, L., Suomalainen, A., Lapatto, R., Kuuluvainen, E., Hietakangas, V., Otonkoski, T., et al. (2024) · The American Journal of Human Genetics · doi:10.1016/j.ajhg.2024.03.004 · Cited by 17 · see citing works

Antisense Oligonucleotide Embedded Context Responsive Nanoparticles Derived from Synthetic Ionizable Lipids for lncRNA Targeted Therapy of Breast Cancer

Sarkar, S., Moitra, P., Bera, S., & Bhattacharya, S. (2024) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.4c04893 · Cited by 17 · see citing works

A Cell-Free Kinetic Analysis of Ionizable Lipid Hydrolysis

Couture‐Senécal, J., Natraj, J., & Khan, O. F. (2024) · Analytical Chemistry · doi:10.1021/acs.analchem.4c02399 · Cited by 16 · see citing works

Potential of siRNA in COVID-19 therapy: Emphasis on in silico design and nanoparticles based delivery

Fopase, R., Panda, C., Rajendran, A. P., Uludağ, H., & Pandey, L. M. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1112755 · Cited by 15 · see citing works

Lipid nanoparticle mediated small interfering RNA delivery as a potential therapy for Alzheimer's disease

Ahmed, T. (2024) · European Journal of Neuroscience · doi:10.1111/ejn.16336 · Cited by 16 · see citing works

CO-DELIVERY of glutamic acid-extended peptide antigen and imidazoquinoline TLR7/8 agonist via ionizable lipid nanoparticles induces protective anti-tumor immunity

Ye, T., Zhong, Z., Cappellesso, F., Deswarte, K., Chen, Y., Lauwers, H., Lombaerde, E. D., Gontsarik, M., Lienenklaus, S., Lysebetten, D. V., et al. (2024) · Biomaterials · doi:10.1016/j.biomaterials.2024.122693 · Cited by 15 · see citing works

Branch‐PCR Constructed Stable shRNA Transcription Nanoparticles Have Long‐Lasting RNAi Effect

Liu, J., Wang, R., Ma, D., Li, Y., Wei, C., & Xi, Z. (2016) · ChemBioChem · doi:10.1002/cbic.201600047 · Cited by 15 · see citing works

Endosomal escape and current obstacles in ionizable lipid nanoparticles mediated gene delivery: lessons from COVID-19 vaccines

Meerasa, S. S., Ahmad, A., Khan, A. A., Haque, S., & Saleem, I. Y. (2025) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2025.126263 · Cited by 14 · see citing works

Navigating the future: Microfluidics charting new routes in drug delivery

Mohammadi, M., Qadir, S. A., Faraj, A. M., Shareef, O. H., Mahmoodi, H., Mahmoudi, F., & Moradi, S. (2024) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2024.124142 · Cited by 14 · see citing works

Poly(β-aminoester) Physicochemical Properties Govern the Delivery of siRNA from Electrostatically Assembled Coatings

Berger, A. G., DeLorenzo, C., Vo, C., Kaskow, J. A., Nabar, N., & Hammond, P. T. (2024) · Biomacromolecules · doi:10.1021/acs.biomac.4c00062 · Cited by 15 · see citing works

Ionizable Amphiphilic Dendrimer‐Based Nanomaterials with Alkyl‐Chain‐Substituted Amines for Tunable siRNA Delivery to the Liver Endothelium In Vivo

Khan, O. F., Zaia, E. W., Yin, H., Bogorad, R. L., Pelet, J. M., Webber, M. J., Zhuang, I., Dahlman, J. E., Langer, R., & Anderson, D. G. (2014) · Angewandte Chemie · doi:10.1002/ange.201408221 · Cited by 14 · see citing works

Construction of Messenger RNA (mRNA) Probes Delivered By Lipid Nanoparticles to Visualize Intracellular Protein Expression and Localization at Organelles

Zhao, W., Zeng, C., Yan, J., Du, S., Hou, X., Zhang, C., Li, W., Deng, B., McComb, D. W., Xue, Y., et al. (2021) · Advanced Materials · doi:10.1002/adma.202103131 · Cited by 14 · see citing works

Engineering ionizable lipids for rapid biodegradation balances mRNA vaccine efficacy and tolerabilityPreprint

Couture‐Senécal, J., Tilstra, G., & Khan, O. F. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.08.02.606386 · Cited by 15 · see citing works

Progress on siRNA-based gene therapy targeting secondary injury after intracerebral hemorrhage

Almarghalani, D. A., & Shah, Z. A. (2021) · Gene Therapy · doi:10.1038/s41434-021-00304-3 · Cited by 13 · see citing works

Optimizing Transfection Efficiency in CAR-T Cell Manufacturing through Multiple Administrations of Lipid-Based Nanoparticles

Giulimondi, F., Digiacomo, L., Renzi, S., Cassone, C., Pirrottina, A., Molfetta, R., Palamà, I. E., Maiorano, G., Gigli, G., Amenitsch, H. W., et al. (2024) · ACS Applied Bio Materials · doi:10.1021/acsabm.4c00103 · Cited by 14 · see citing works

Noncationic polymer-assisted carrier for nucleic acid drug delivery

Su, M., Chen, J., Zhu, Y., Chen, C., Zhang, Y., & Yang, X. (2024) · Science China Materials · doi:10.1007/s40843-024-3048-0 · Cited by 13 · see citing works

Development and applications of lipid hydrophilic headgroups for nucleic acid therapy

Ma, W., Fu, X., Zhao, T., Qi, Y., Zhang, S., & Zhao, Y. (2024) · Biotechnology Advances · doi:10.1016/j.biotechadv.2024.108395 · Cited by 12 · see citing works

The engineering challenges and opportunities when designing potent ionizable materials for the delivery of ribonucleic acids

Lau, Y. M. A., Pang, J., Tilstra, G., Couture‐Senécal, J., & Khan, O. F. (2022) · Expert Opinion on Drug Delivery · doi:10.1080/17425247.2022.2144827 · Cited by 11 · see citing works

pH-sensitive, tail-modified, ester-linked ionizable cationic lipids for gene delivery

Zou, Y., Zhen, Y., Zhao, Y., Chen, H., Wang, R., Wang, W., Ma, P., Zhi, D., Ju, B., & Zhang, S. (2022) · Biomaterials Advances · doi:10.1016/j.bioadv.2022.212984 · Cited by 10 · see citing works

Relationship Between the Physicochemical Properties of Lipid Nanoparticles and the Quality of siRNA Delivery to Liver Cells

Sato, Y., Hatakeyama, H., Hyodo, M., & Harashima, H. (2015) · Molecular Therapy · doi:10.1038/mt.2015.222 · Cited by 85 · see citing works

Understanding structure-activity relationships of pH-sensitive cationic lipids facilitates the rational identification of promising lipid nanoparticles for delivering siRNAs in vivo

Sato, Y., Hashiba, K., Sasaki, K., Maeki, M., Tokeshi, M., & Harashima, H. (2019) · Journal of Controlled Release · doi:10.1016/j.jconrel.2019.01.001 · Cited by 180 · see citing works

* Corresponding author:

Satoa, Y., Hatakeyamaa, H., Sakuraia, Y., Hyodoa, M., Akitaa, H., Harashimaa, H., & Phd, H. H. (2016)

Effect of PEGylation on Biodistribution and Gene Silencing of siRNA/Lipid Nanoparticle Complexes

Bao, Y., Jin, Y., Chivukula, P., Zhang, J., Liu, Y., Liu, J., Clamme, J., Mahato, R. I., Ng, D., Ying, W., et al. (2012) · Pharmaceutical Research · doi:10.1007/s11095-012-0874-6 · Cited by 107 · see citing works

Development of lipid nanoparticle formulations of siRNA for hepatocyte gene silencing following subcutaneous administration

Chen, S., Tam, Y. Y. C., Lin, P. J. C., Leung, A. K. K., Tam, Y. K., & Cullis, P. R. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.09.025 · Cited by 160 · see citing works