The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Exploring the Trans‐Cleavage Activity of CRISPR‐Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor
Open accessAbstract
An accurate, rapid, and cost‐effective biosensor for the quantification of disease biomarkers is vital for the development of early‐diagnostic point‐of‐care systems. The recent discovery of the trans‐cleavage property of CRISPR type V effectors makes CRISPR a potential high‐accuracy bio‐recognition tool. Herein, a CRISPR‐Cas12a (cpf1) based electrochemical biosensor (E‐CRISPR) is reported, which is more cost‐effective and portable than optical‐transduction‐based biosensors. Through optimizing the in vitro trans‐cleavage activity of Cas12a, E‐CRIPSR was used to detect viral nucleic acids, including human papillomavirus 16 (HPV‐16) and parvovirus B19 (PB‐19), with a picomolar sensitivity. An aptamer‐based E‐CRISPR cascade was further designed for the detection of transforming growth factor β1 (TGF‐β1) protein in clinical samples. As demonstrated, E‐CRISPR could enable the development of portable, accurate, and cost‐effective point‐of‐care diagnostic systems.
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Cites
Multiplex Genome Engineering Using CRISPR/Cas Systems
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CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
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Cited by
Kaminski, M. M., Abudayyeh, O. O., Gootenberg, J. S., Zhang, F., & Collins, J. J. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00760-7 · Cited by 1,259
Device integration of electrochemical biosensors
Wu, J., Liu, H., Chen, W., Ma, B., & Ju, H. (2023) · Nature Reviews Bioengineering · doi:10.1038/s44222-023-00032-w · Cited by 895
CRISPR-Cas12a-driven MXene-PEDOT:PSS piezoresistive wireless biosensor
Zeng, R., Wang, W., Chen, M., Wan, Q., Wang, C., Knopp, D., & Tang, D. (2021) · Nano Energy · doi:10.1016/j.nanoen.2020.105711 · Cited by 394
Identifying Selectivity Filters in Protein Biosensor for Ligand Screening
Sahil, M., Singh, J., Sahu, S., Pal, S. K., Yadav, A., Anand, R., & Mondal, J. (2023) · JACS Au · doi:10.1021/jacsau.3c00374 · Cited by 6
Metadata
- Authors
- Dai, Y., Somoza, R. A., Wang, L., Welter, J. F., Li, Y., Caplan, A. I., & Liu, C. C.
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2019-10-17
- Volume
- 58
- Issue
- 48
- Pages
- 17399-17405
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- OpenAlex
- W2978292797
- Cited by
- 660
- References
- 10
- FWCI
- 19.05
- Type
- journal article
- Language
- en
- Open access
- green
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- Dai, Y., Somoza, R. A., Wang, L., Welter, J. F., Li, Y., Caplan, A. I., & Liu, C. C. (2019). Exploring the Trans‐Cleavage Activity of CRISPR‐Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor. Angewandte Chemie International Edition. https://doi.org/10.1002/anie.201910772
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