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Visualization of biomass solubilization and cellulose regeneration during ionic liquid pretreatment of switchgrass

Open access
Singh, Seema, Simmons, Blake A., Vogel, Kenneth P.·2009·Biotechnology and Bioengineering

Abstract

Auto‐fluorescent mapping of plant cell walls was used to visualize cellulose and lignin in pristine switchgrass (Panicum virgatum) stems to determine the mechanisms of biomass dissolution during ionic liquid pretreatment. The addition of ground switchgrass to the ionic liquid 1‐n‐ethyl‐3‐methylimidazolium acetate resulted in the disruption and solubilization of the plant cell wall at mild temperatures. Swelling of the plant cell wall, attributed to disruption of inter‐ and intramolecular hydrogen bonding between cellulose fibrils and lignin, followed by complete dissolution of biomass, was observed without using imaging techniques that require staining, embedding, and processing of biomass. Subsequent cellulose regeneration via the addition of an anti‐solvent, such as water, was observed in situ and provided direct evidence of significant rejection of lignin from the recovered polysaccharides. This observation was confirmed by chemical analysis of the regenerated cellulose. In comparison to untreated biomass, ionic liquid pretreated biomass produces cellulose that is efficiently hydrolyzed with commercial cellulase cocktail with high sugar yields over a relatively short time interval. Biotechnol. Bioeng. 2009; 104: 68–75 Published 2009 Wiley Periodicals, Inc.

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Metadata

Authors
Singh, S., Simmons, B. A., & Vogel, K. P.
Venue
Biotechnology and Bioengineering
Publisher
Wiley
Published
2009-06
Volume
104
Issue
1
Pages
68-75
ISSN
  • 0006-3592 (ISSN-L)
  • 1097-0290
OpenAlex
W2122333067
Cited by
379
References
8
FWCI
21.54
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Visualization of biomass solubilization and cellulose regeneration during ionic liquid pretreatment of switchgrass. https://app.cochat.ai/papers/10.1002/bit.22386
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Singh, S., Simmons, B. A., & Vogel, K. P. (2009). Visualization of biomass solubilization and cellulose regeneration during ionic liquid pretreatment of switchgrass. Biotechnology and Bioengineering. https://doi.org/10.1002/bit.22386

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