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Cited by 355

The DOI matches its Crossref record; 124 of the 124 references it cites were found in a registry or an index.

Bioengineering virus‐like particles as vaccines

Lua, Linda H.L., Connors, Natalie K., Sainsbury, Frank, Chuan, Yap P., Wibowo, Nani, Middelberg, Anton P.J.·2014·Biotechnology and Bioengineering
DOI 10.1002/bit.25159
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Abstract

Virus‐like particle (VLP) technology seeks to harness the optimally tuned immunostimulatory properties of natural viruses while omitting the infectious trait. VLPs that assemble from a single protein have been shown to be safe and highly efficacious in humans, and highly profitable. VLPs emerging from basic research possess varying levels of complexity and comprise single or multiple proteins, with or without a lipid membrane. Complex VLP assembly is traditionally orchestrated within cells using black‐box approaches, which are appropriate when knowledge and control over assembly are limited. Recovery challenges including those of adherent and intracellular contaminants must then be addressed. Recent commercial VLPs variously incorporate steps that include VLP in vitro assembly to address these problems robustly, but at the expense of process complexity. Increasing research activity and translation opportunity necessitate bioengineering advances and new bioprocessing modalities for efficient and cost‐effective production of VLPs. Emerging approaches are necessarily multi‐scale and multi‐disciplinary, encompassing diverse fields from computational design of molecules to new macro‐scale purification materials. In this review, we highlight historical and emerging VLP vaccine approaches. We overview approaches that seek to specifically engineer a desirable immune response through modular VLP design, and those that seek to improve bioprocess efficiency through inhibition of intracellular assembly to allow optimal use of existing purification technologies prior to cell‐free VLP assembly. Greater understanding of VLP assembly and increased interdisciplinary activity will see enormous progress in VLP technology over the coming decade, driven by clear translational opportunity. Biotechnol. Bioeng. 2014;111: 425–440. © 2013 Wiley Periodicals, Inc.

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Metadata

Authors
Lua, L. H. L., Connors, N. K., Sainsbury, F., Chuan, Y. P., Wibowo, N., & Middelberg, A. P. J.
Venue
Biotechnology and Bioengineering
Publisher
Wiley
Published
2013-12-17
Volume
111
Issue
3
Pages
425-440
ISSN
  • 0006-3592 (ISSN-L)
  • 1097-0290
DOI
10.1002/bit.25159
OpenAlex
W2146700406
Cited by
355
References
124
FWCI
11.43
Type
journal article
Language
en
Open access
closed

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