Cited by 110Analytical Chemistry and ChromatographyAnalytical Methods in PharmaceuticalsMetabolomics and Mass Spectrometry Studies

Simultaneous determination of lactate and pyruvate in human sweat using reversed‐phase high‐performance liquid chromatography: a noninvasive approach

Biagi, Simona, Ghimenti, Silvia, Onor, Massimo, Bramanti, Emilia·2012·Biomedical Chromatography

Abstract

The simultaneous determination of lactate and pyruvate in sweat has been performed using reversed phase high‐performance liquid chromatography (RP‐HPLC) with UV detection at 220 nm. The calibration curves were linear in the investigated range 0.3 ‐ 350 m m of lactate, 0.003‐ 1 m m of pyruvate. The sensitivity was good with a limit of detection of 0.03 m m for lactate and 0.001 m m for pyruvate. Recoveries evaluated for the entire procedure were 102 ± 0.1 and 96 ± 0.1for lactate and pyruvate, respectively. The method was successfully applied to analysis of sweat in 8 athletes at rest (pilocarpine sweating) and during physical exercise. Copyright © 2012 John Wiley & Sons, Ltd.

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Crossref · 10.1002/bmc.2713

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Authors
Biagi, S., Ghimenti, S., Onor, M., & Bramanti, E.
Affiliations
  • Institute for Chemical and Physical Processes (IT)
  • University of Pisa (IT)
  • Institute for the Chemistry of OrganoMetallic Compounds (IT)
Corresponding author
Bramanti, Emilia
Venue
Biomedical Chromatography
Publisher
Wiley
Published
2012-02-07
Volume
26
Issue
11
Pages
1408-1415
ISSN
  • 0269-3879 (ISSN-L)
  • 1099-0801
OpenAlex
W1541040341
Cited by
110
References
51
Citations by year
2026: 4 · 2025: 22 · 2024: 11 · 2023: 12 · 2022: 15 · 2021: 11 · 2020: 11 · 2019: 7 · 2018: 2 · 2017: 5 · 2016: 4 · 2015: 3 · 2014: 1 · 2013: 2
FWCI
0.85
Type
journal article
Language
en
Open access
closed
Locations
  • Biomedical Chromatography (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Keywords
lactate, pyruvate, human sweat, non-invasive monitoring, exercise physiology, athletes, simultaneous determination
MeSH terms
Adult, Chromatography, High Pressure Liquid, Female, Humans, Male, Rest, Sensitivity and Specificity, Sweat, Reproducibility of Results, Exercise, Linear Models, Pyruvic Acid, Lactic Acid, Chromatography, Reverse-Phase

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