Simultaneous determination of lactate and pyruvate in human sweat using reversed‐phase high‐performance liquid chromatography: a noninvasive approach
Abstract
The simultaneous determination of lactate and pyruvate in sweat has been performed using reversed phase high‐performance liquid chromatography (RP‐HPLC) with UV detection at 220 nm. The calibration curves were linear in the investigated range 0.3 ‐ 350 m m of lactate, 0.003‐ 1 m m of pyruvate. The sensitivity was good with a limit of detection of 0.03 m m for lactate and 0.001 m m for pyruvate. Recoveries evaluated for the entire procedure were 102 ± 0.1 and 96 ± 0.1for lactate and pyruvate, respectively. The method was successfully applied to analysis of sweat in 8 athletes at rest (pilocarpine sweating) and during physical exercise. Copyright © 2012 John Wiley & Sons, Ltd.
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The concentration of lactate in sweat is an indicator of exercise intolerance and tissue hypoxia.
“The concentration of lactate in sweat is an indicator of exercise intolerance and tissue hypoxia (44, 45).”
From A soft, wearable microfluidic device for the capture, storage, and colorimetric sensing of sweat (2016) · cites this paper as [45]
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A soft, wearable microfluidic device for the capture, storage, and colorimetric sensing of sweat
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- Authors
- Biagi, S., Ghimenti, S., Onor, M., & Bramanti, E.
- Affiliations
- Institute for Chemical and Physical Processes (IT)
- University of Pisa (IT)
- Institute for the Chemistry of OrganoMetallic Compounds (IT)
- Corresponding author
- Bramanti, Emilia
- Venue
- Biomedical Chromatography
- Publisher
- Wiley
- Published
- 2012-02-07
- Volume
- 26
- Issue
- 11
- Pages
- 1408-1415
- ISSN
- 0269-3879 (ISSN-L)
- 1099-0801
- OpenAlex
- W1541040341
- Cited by
- 110
- References
- 51
- Citations by year
- 2026: 4 · 2025: 22 · 2024: 11 · 2023: 12 · 2022: 15 · 2021: 11 · 2020: 11 · 2019: 7 · 2018: 2 · 2017: 5 · 2016: 4 · 2015: 3 · 2014: 1 · 2013: 2
- FWCI
- 0.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomedical Chromatography (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- lactate, pyruvate, human sweat, non-invasive monitoring, exercise physiology, athletes, simultaneous determination
- MeSH terms
- Adult, Chromatography, High Pressure Liquid, Female, Humans, Male, Rest, Sensitivity and Specificity, Sweat, Reproducibility of Results, Exercise, Linear Models, Pyruvic Acid, Lactic Acid, Chromatography, Reverse-Phase
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