LC/MS/MS analysis of the endogenous dimethyltryptamine hallucinogens, their precursors, and major metabolites in rat pineal gland microdialysate.
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We report a qualitative liquid chromatography-tandem mass spectrometry (LC/MS/MS) method for the simultaneous analysis of the three known N,N-dimethyltryptamine endogenous hallucinogens, their precursors and metabolites, as well as melatonin and its metabolic precursors. The method was characterized using artificial cerebrospinal fluid (aCSF) as the matrix and was subsequently applied to the analysis of rat brain pineal gland-aCSF microdialysate. The method describes the simultaneous analysis of 23 chemically diverse compounds plus a deuterated internal standard by direct injection, requiring no dilution or extraction of the samples. The results demonstrate that this is a simple, sensitive, specific and direct approach to the qualitative analysis of these compounds in this matrix. The protocol also employs stringent MS confirmatory criteria for the detection and confirmation of the compounds examined, including exact mass measurements. The excellent limits of detection and broad scope make it a valuable research tool for examining the endogenous hallucinogen pathways in the central nervous system. We report here, for the first time, the presence of N,N-dimethyltryptamine in pineal gland microdialysate obtained from the rat.
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DMT is present in mammalian organisms, including human blood, brain, and cerebrospinal fluid, and in the rat pineal gland.
“The hallucinogenic component DMT is abundant in the plant kingdom (Khan et al., 2012) and it is also present in mammalian organism; studies have detected it in human blood, brain, cerebrospinal fluid (Wallach, 2009), and the pineal gland of rats (Barker et al., 2013).”
From The therapeutic potentials of Ayahuasca: possible effects against various diseases of civilization (2016) · cites this paper as Barker et al., 2013
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Self-citation
- Authors
- Barker, S. A., Borjigin, J., Lomnicka, I., & Strassman, R.
- Affiliations
- Louisiana State University (US)
- University of Michigan (US)
- University of New Mexico (US)
- Corresponding author
- Barker, Steven A.
- Venue
- Biomedical Chromatography
- Publisher
- Wiley
- Published
- 2013-07-23
- Volume
- 27
- Issue
- 12
- Pages
- 1690-1700
- ISSN
- 0269-3879 (ISSN-L)
- 1099-0801
- OpenAlex
- W2120522320
- Cited by
- 72
- References
- 32
- Citations by year
- 2026: 2 · 2025: 10 · 2024: 5 · 2023: 5 · 2022: 11 · 2021: 2 · 2020: 8 · 2019: 8 · 2018: 5 · 2017: 3 · 2016: 4 · 2015: 4 · 2014: 5
- FWCI
- 4.82
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biomedical Chromatography (publishedVersion)
- PubMed (publishedVersion)
- Deep Blue (University of Michigan) (submittedVersion)
- Topics
- Social Sciences › Psychology › Clinical Psychology › Psychedelics and Drug Studies
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Forensic Toxicology and Drug Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- N,N-dimethyltryptamine, pineal gland, melatonin, melatonin precursors, microdialysate, artificial cerebrospinal fluid, LC-MS/MS method development, deuterated internal standards, rat brain
- MeSH terms
- Animals, Chromatography, Liquid, N,N-Dimethyltryptamine, Drug Stability, Hallucinogens, Male, Pineal Gland, Rats, Wistar, Microdialysis, Rats, Tandem Mass Spectrometry, Limit of Detection
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