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Cited by 22MicroRNA in disease regulationTreatment of Major DepressionNeurogenesis and neuroplasticity mechanisms

MiRNA‐199a‐5p targets WNT2 to regulate depression through the CREB/BDNF signaling in hippocampal neuron

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Liu, Zheng, Yang, Jianli, Fang, Qing, Shao, Hua, Yang, Dalu, Sun, Junfang, Gao, Lizhi·2021·Brain and Behavior
DOI 10.1002/brb3.2107PMC PMC8413827
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Abstract

Introduction: This study mainly investigated the role of miR‐199a‐5p in depression. Methods: qRT‐PCR and western blotting were employed to detect the expressions of miR‐199a‐5p, CREB and BDNF. Sucrose preference test, forced swimming test, and tail suspension test were performed to evaluate depression‐related symptoms. MTT assays and flow cytometry were used to examine the cell reproduction and apoptotic cells of hippocampal neuron. Results: The data demonstrated that the expression levels of miR‐199a‐5p in the cerebrospinal fluids and serums of depression patient and the hippocampus of chronic unpredictable mild stress (CUMS) mouse were significantly increased. However, the expressions of WNT2, p‐CREB, and BDNF were inhibited. In addition, miR‐199a‐5p‐inhibitor enhanced sucrose preferences of CUMS mouse and decreased immobile time in sucrose preference test and forced swimming test. Knockdown of WNT2 attenuated the effects of miR‐199a‐5p‐inhibitor on cell reproduction and apoptotic cells of hippocampal neuron and the expression of WNT2, p‐CREB, and BDNF. Conclusion: MiR‐199a‐5p can target WNT2 to enhance the development of depression through regulation of the CREB/BDNF signaling. Trial registration: JNU‐Hos‐49284.

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  • In depression, more miR-199a-5p was noted in microglia, suppressing WNT2 expression through CREB/BDNF signaling regulation.

    “In depression, more miR-199a-5p was noted in microglia suppressing WNT2 expression through the CREB/BDNF signaling regulation.270”

    From Epigenetic regulation in major depression and other stress-related disorders: molecular mechanisms, clinical relevance and therapeutic potential (2023) · cites this paper as [270]

    The Abstract Results report increased miR-199a-5p in patients’ cerebrospinal fluid and serum and in the hippocampus of depressed mice. The Conclusions state that miR-199a-5p targets WNT2 through CREB/BDNF signaling, but the paper studies hippocampal neurons, not increased miR-199a-5p in microglia. Page numbers are unavailable in our copy. (LLM, conf=0.90)

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Cited by

Sorted by citations

Authors
Liu, Z., Yang, J., Fang, Q., Shao, H., Yang, D., Sun, J., & Gao, L.
Affiliations
  • Baotou Medical College (CN)
  • Tianjin Medical University General Hospital (CN)
  • Department of department of neurology The Second Affiliated Hospital of Baotou Medical College Baotou China
Corresponding author
Yang, Jianli
ORCID
Venue
Brain and Behavior
Publisher
Wiley
Published
2021-08
Volume
11
Issue
8
Pages
e02107-e02107
ISSN
  • 2157-9032 (ISSN-L)
  • 2162-3279
DOI
10.1002/brb3.2107
PMC
PMC8413827
OpenAlex
W3192802547
Cited by
22
References
6
Citations by year
2026: 1 · 2025: 5 · 2024: 7 · 2023: 6 · 2022: 3
FWCI
1.59
Type
journal article
Language
en
Open access
Licence
Locations
  • Brain and Behavior (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Health Sciences › Medicine › Pharmacology › Treatment of Major Depression
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
miR-199a-5p, Wnt2, depression, CREB/BDNF signaling, hippocampal neurons, chronic unpredictable mild stress, neuronal apoptosis, microRNA target regulation, depression biomarkers
MeSH terms
Animals, Depression, Hippocampus, Humans, Neurons, Brain-Derived Neurotrophic Factor, MicroRNAs, Wnt2 Protein, Mice

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