Cited by 71Pain Mechanisms and TreatmentsNeuroscience and Neuropharmacology ResearchIon Channels and Receptors

Morphological and functional characterization of cholinergic interneurons in the dorsal horn of the mouse spinal cord

Mesnage, Bruce, Gaillard, Stéphane, Godin, Antoine G., Rodeau, Jean‐Luc, Hammer, Matthieu, Von Engelhardt, Jakob, Wiseman, Paul W., De Koninck, Yves +2 more·2011·Journal of Comparative Neurology

Abstract

Endogenous acetylcholine is an important modulator of sensory processing, especially at the spinal level, where nociceptive (pain‐related) stimuli enter the central nervous system and are integrated before being relayed to the brain. To decipher the organization of the local cholinergic circuitry in the spinal dorsal horn, we used transgenic mice expressing enchanced green fluorescent protein specifically in cholinergic neurons (ChAT::EGFP) and characterized the morphology, neurochemistry, and firing properties of the sparse population of cholinergic interneurons in this area. Three‐dimensional reconstruction of lamina III ChAT::EGFP neurons based either on their intrinsic fluorescence or on intracellular labeling in live tissue demonstrated that these neurons have long and thin processes that grow preferentially in the dorsal direction. Their dendrites and axon are highly elongated in the rostrocaudal direction, beyond the limits of a single spinal segment. These unique morphological features suggest that dorsal horn cholinergic interneurons are the main contributors to the plexus of cholinergic processes located in lamina IIi, just dorsal to their cell bodies. In addition, immunostainings demonstrated that dorsal horn cholinergic interneurons in the mouse are γ‐aminobutyric acidergic and express nitric oxide synthase, as in rats. Finally, electrophysiological recordings from these neurons in spinal cord slices demonstrate that two‐thirds of them have a repetitive spiking pattern with frequent rebound spikes following hyperpolarization. Altogether our results indicate that, although they are rare, the morphological and functional features of cholinergic neurons enable them to collect segmental information in superficial layers of the dorsal horn and to modulate it over several segments. J. Comp. Neurol. 519:3139–3158, 2011. © 2011 Wiley‐Liss, Inc.

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Crossref · 10.1002/cne.22668

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  • The Chat dorsal inhibitory cluster Inhib-14 was a deep dorsal (lamina 4), inhibitory and cholinergic population that also expressed Nos1.

    “The Chat dorsal inhibitor cluster Inhib-14 was a deep dorsal (lamina 4), inhibitory and cholinergic population and also expressed Nos180–82.”

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Authors
Mesnage, B., Gaillard, S., Godin, A. G., Rodeau, J., Hammer, M., Von Engelhardt, J., Wiseman, P. W., De Koninck, Y., Schlichter, R., & Cordero‐Erausquin, M.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • McGill University (CA)
Corresponding author
Cordero‐Erausquin, Matilde
Venue
Journal of Comparative Neurology
Publisher
Wiley
Published
2011-09-14
Volume
519
Issue
16
Pages
3139-3158
ISSN
  • 0021-9967 (ISSN-L)
  • 1096-9861
OpenAlex
W2036117048
Cited by
71
References
70
Citations by year
2026: 3 · 2025: 7 · 2024: 2 · 2023: 2 · 2022: 5 · 2021: 7 · 2020: 7 · 2019: 1 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 8 · 2014: 5 · 2013: 7 · 2012: 6
FWCI
3.87
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Comparative Neurology (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Funders
  • Agence Nationale de la Recherche
  • Canadian Institutes of Health Research
Topics
  • Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
Keywords
cholinergic interneurons, dorsal horn, spinal cord, acetylcholine, lamina III, neuronal morphology, GABAergic neurons, nitric oxide synthase, nociception, pain processing, 3D reconstruction
MeSH terms
Acetylcholine, Animals, Interneurons, Male, Mice, Transgenic, Posterior Horn Cells, Mice

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