Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications
Abstract
Capillary electrophoresis (CE) offers fast and high‐resolution separation of charged analytes from small injection volumes. Coupled to mass spectrometry (MS), it represents a powerful analytical technique providing (exact) mass information and enables molecular characterization based on fragmentation. Although hyphenation of CE and MS is not straightforward, much emphasis has been placed on enabling efficient ionization and user‐friendly coupling. Though several interfaces are now commercially available, research on more efficient and robust interfacing with nano‐electrospray ionization (ESI), matrix‐assisted laser desorption/ionization (MALDI) and inductively coupled plasma mass spectrometry (ICP) continues with considerable results. At the same time, CE‐MS has been used in many fields, predominantly for the analysis of proteins, peptides and metabolites. This review belongs to a series of regularly published articles, summarizing 248 articles covering the time between June 2016 and May 2018. Latest developments on hyphenation of CE with MS as well as instrumental developments such as two‐dimensional separation systems with MS detection are mentioned. Furthermore, applications of various CE‐modes including capillary zone electrophoresis (CZE), nonaqueous capillary electrophoresis (NACE), capillary gel electrophoresis (CGE) and capillary isoelectric focusing (CIEF) coupled to MS in biological, pharmaceutical and environmental research are summarized.
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Recent advances in CE-MS platforms have attracted considerable interest from the proteomics community as an alternative to LC-MS.
“Recent advances in CE-MS platforms have attracted considerable interest from the proteomics community as an alternative to LC-MS9–11.”
From Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research (2019) · cites this paper as [10]
CZE is the most commonly used CE technique in proteomics research.
“While many CE-based techniques have been reported for the analysis of proteins and peptides, CZE is the most commonly used in proteomics research10,64–66.”
From Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research (2019) · cites this paper as [10]
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Cites
Dai, J., Lamp, J., Xia, Q., & Zhang, Y. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.7b04608 · Cited by 123 · see citing works
Jooß, K., Hühner, J., Kiessig, S., Moritz, B., & Neusüß, C. (2017) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-017-0542-0 · Cited by 79 · see citing works
Self-citationGiorgetti, J., D’Atri, V., Canonge, J., Lechner, A., Guillarme, D., Colas, O., Wagner-Rousset, E., Beck, A., Leize-Wagner, E., & François, Y.-N. (2018) · Talanta · doi:10.1016/j.talanta.2017.09.083 · Cited by 65 · see citing works
Capillary electrophoresis–mass spectrometry for direct structural identification of serum N-glycans
Snyder, C. M., Zhou, X., Karty, J. A., Fonslow, B. R., Novotny, M. V., & Jacobson, S. C. (2017) · Journal of Chromatography A · doi:10.1016/j.chroma.2017.09.009 · Cited by 58 · see citing works
Predicting Electrophoretic Mobility of Tryptic Peptides for High-Throughput CZE-MS Analysis
Krokhin, O. V., Anderson, G., Spicer, V., Sun, L., & Dovichi, N. J. (2017) · Analytical Chemistry · doi:10.1021/acs.analchem.6b04544 · Cited by 53 · see citing works
Chen, D., Shen, X., & Sun, L. (2018) · Analytica Chimica Acta · doi:10.1016/j.aca.2018.01.037 · Cited by 47 · see citing works
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Urinary CE-MS peptide marker pattern for detection of solid tumors
Belczacka, I., Latosinska, A., Siwy, J., Metzger, J., Merseburger, A. S., Mischak, H., Vlahou, A., Frantzi, M., & Jankowski, V. (2018) · Scientific Reports · doi:10.1038/s41598-018-23585-y · Cited by 37 · see citing works
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Self-citationMarie, A.-L., Dominguez-Vega, E., Saller, F., Plantier, J.-L., Urbain, R., Borgel, D., Tran, N. T., Somsen, G. W., & Taverna, M. (2016) · Analytica Chimica Acta · doi:10.1016/j.aca.2016.10.016 · Cited by 26 · see citing works
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Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research
Gomes, F. P., & Yates, J. R. (2019) · Mass Spectrometry Reviews · doi:10.1002/mas.21599 · Cited by 112 · see citing works
- Authors
- Stolz, A., Jooß, K., Höcker, O., Römer, J., Schlecht, J., & Neusüß, C.
- Affiliations
- Hochschule Aalen (DE)
- Helmholtz Munich (DE)
- Corresponding author
- Neusüß, Christian
- Venue
- ELECTROPHORESIS
- Publisher
- Wiley
- Published
- 2018-11-12
- Volume
- 40
- Issue
- 1
- Pages
- 79-112
- ISSN
- 0173-0835 (ISSN-L)
- 1522-2683
- OpenAlex
- W2892499411
- Cited by
- 214
- References
- 17
- Citations by year
- 2026: 15 · 2025: 29 · 2024: 18 · 2023: 30 · 2022: 40 · 2021: 38 · 2020: 30 · 2019: 13
- FWCI
- 1.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Electrophoresis (publishedVersion)
- PubMed (publishedVersion)
- OPUS (Aalen University) (submittedVersion)
- PuSH - Publication Server of Helmholtz Zentrum München (submittedVersion)
- Funders
- Roche
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- capillary electrophoresis-mass spectrometry, nanoelectrospray ionization, MALDI, inductively coupled plasma mass spectrometry, two-dimensional separation, capillary zone electrophoresis, nonaqueous capillary electrophoresis, capillary gel electrophoresis, capillary isoelectric focusing, protein analysis, peptide analysis, metabolite analysis, environmental analysis, pharmaceutical analysis
- MeSH terms
- Animals, Humans, Mass Spectrometry, Biomarkers, Electrophoresis, Capillary, Mice, Metabolomics
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