Dye affinity chromatography for fast and simple purification of fucoidan from marine brown algae
Open accessAbstract
Fucoidan is a sulfated polysaccharide with promising pharmacological applications. Due to its medicinal properties, there is a demand for a separation technique that yields a high purification grade. Here, we present a novel purification tool for recovering fucoidan from the marine brown macroalgae Fucus vesiculosus. The developed method is based on amino‐derivatized Sepabeads® EC‐EA. The beads were modified with toluidine blue (TB), a thiazine derivative, to exploit the strong donor acceptor interactions between the cationic dye and the anionic polysaccharide. The adsorption kinetics and the binding capacity of the resin were analyzed. A Sips model was used to approximate the adsorption isotherm, resulting in a maximum capacity of 127.7 mg fucoidan per g adsorbent. Investigation of the effect of adsorption step's pH on purity and chemical structure was performed by TB and Fourier transform infra‐red spectroscopy assays. Results showed that adsorption at pH 1 and 6 had negligible effects on fucoidan's chemical structure. However, purity was actually improved by 1.55‐ and 1.69‐fold at pH 1 and 6, respectively, with an average yield of 5 g/100 g dried algae powder. In contrast, only a 1.46‐fold increment was observed in fucoidan purified by the traditional method at pH 2, with a yield of 7.5 g/100 g dried algae powder. Furthermore, fucoidan purified by this method at pH 6 complies with, or even exceeds the quality of the commercially available (≥95% pure) fucoidan (Sigma‐Aldrich®) with respect to molecular weight and sulfur content. Therefore, dye affinity chromatography provides more advantages than the classically used techniques for fucoidan purification.
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Fucose-binding lectins can purify fucoidan from crude solutions, but sulphate content may block fucose units and interfere.
“In the case of polysaccharides, fucose-binding lectins were used effectively to purify fucoidan from crude solutions; however problems could arise if the sulphate content of the polysaccharide blocks the fucose units that interact with the lectins”
From Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification (2017) · cites this paper as Hahn et al., 2016
A new dye-affinity chromatography method using modified amino-derivatized sepabeads with toluidine blue purified fucoidan from brown macroalgae at higher purity than commercial purified fucoidan from Sigma-Aldrich (95%).
“Recently, Hahn et al. (2016) developed a new dye-affinity chromatography method using modified amino-derivatized sepabeads with toluidine blue, showing promising results in the purification of fucoidan from brown macroalgae at higher purity levels than the commercially available purified fucoidan from Sigma-Aldrich (95%).”
From Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification (2017) · cites this paper as Hahn et al., 2016
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- Authors
- Hahn, T., Zayed, A., Kovacheva, M., Stadtmüller, R., Lang, S., Muffler, K., & Ulber, R.
- Affiliations
- Fraunhofer Institute for Interfacial Engineering and Biotechnology (DE)
- University of Kaiserslautern (DE)
- Technische Universität Braunschweig (DE)
- TH Bingen University of Applied Sciences (DE)
- Corresponding author
- Ulber, Roland
- Venue
- Engineering in Life Sciences
- Publisher
- Wiley
- Published
- 2015-10-13
- Volume
- 16
- Issue
- 1
- Pages
- 78-87
- ISSN
- 1618-0240 (ISSN-L)
- 1618-2863
- OpenAlex
- W2143585068
- Cited by
- 40
- References
- 22
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 5 · 2023: 5 · 2022: 3 · 2021: 3 · 2020: 5 · 2019: 4 · 2018: 1 · 2017: 4 · 2016: 4
- FWCI
- 4.67
- Type
- journal article
- Language
- en
- Open access
- green
- doaj.org
- Licence
- Locations
- Engineering in Life Sciences (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Publikationsdatenbank der Fraunhofer-Gesellschaft (Fraunhofer-Gesellschaft) (submittedVersion)
- Fraunhofer-Publica (Fraunhofer-Gesellschaft) (submittedVersion)
- Funders
- DFG (UL 170/3-3, LA 829/5-2)
- Ministry of higher education Egypt (57076387)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Keywords
- fucoidan, dye affinity chromatography, Fucus vesiculosus, brown macroalgae, sulfated polysaccharides, toluidine blue, adsorption isotherms, Sips isotherm, polysaccharide purification, molecular weight, sulfur content
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