Isolation and Culture of a Marine Bacterium Degrading the Sulfated Fucans from Marine Brown Algae
Open accessAbstract
Fucoidans are matrix polysaccharides from marine brown algae, consisting of an alpha-L-fucose backbone substituted by sulfate-ester groups and masked with ramifications containing other monosaccharide residues. In spite of their interest as biologically active compounds in a number of homologous and heterologous systems, no convenient sources with fucanase activity are available yet for the degradation of the fucalean algae. We here report on the isolation, characterization, and culture conditions of a bacterial strain capable of degrading various brown algal fucoidans. This bacterium, a member of the family Flavobacteriaceae, was shown to secrete fucoidan endo-hydrolase activity. An extracellular enzyme preparation was used to degrade the fucoidan from the brown alga Pelvetia canaliculata. End products included a tetrasaccharide and a hexasaccharide made of the repetition of disaccharidic units consisting of alpha-1-->3-L-fucopyranose-2-sulfate-alpha-1-->4-L-fucopyranose-2,3-disulfate, with the 3-linked residues at the nonreducing end.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Fucoidanase activities were first detected in several different marine microorganisms, including marine bacteria such as Vibrio sp., Alteromonas sp., Pseudoalteromonas sp., and bacteria of the family Flavobacteriaceae.
“Fucoidanase activities were first detected in several different marine microorganisms, including marine bacteria such as Vibrio sp., Alteromonas sp., Pseudoalteromonas sp., and bacteria of the family Flavobacteriaceae.32,36,37,43”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [32]
One type of fucoidanase catalyses cleavage of small, low molecular weight oligosaccharides from the end of fucoidan backbone chains.
“one type of fucoidanase catalyses the cleavage of small, low molecular weight oligosaccharides from the end of the fucoidan backbone chains”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [32]
Another type of fucoidanase catalyses glycosidic bonds (both α(1→3)- and α(1→4)-bonds) in the fucose-backbone via random endo-type hydrolysis.
“the other type of fucoidanase catalyses the glycosidic bonds (both a(1A3)-, a(1A4)-bonds) in the fucose-back bone via random endo-type hydrolysis”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [32]
α-L-fucosidase only catalyses cleavage of α-L-fucosyl linkages at the nonreducing termini, releasing fucose from the fucose-backbone structure in fucoidans.
“a-L-fucosidase only catalyses the cleavage the a-L-fucosyl linkages at the nonreducing termini, releasing fucose from the fucose-backbone structure in fucoidans (Enzyme type 3, Fig. 1).”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [32]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589 · see citing works
Molecular Cloning. A Laboratory Manual
Wood, E. (1983) · Biochemical Education · doi:10.1016/0307-4412(83)90068-7 · Cited by 29,534 · see citing works
New method for quantitative determination of uronic acids
Blumenkrantz, N., & Asboe-Hansen, G. (1973) · Analytical Biochemistry · doi:10.1016/0003-2697(73)90377-1 · Cited by 7,140 · see citing works
Manual of Methods for General Bacteriology
Jones, D. (1981) · Journal of Clinical Pathology · doi:10.1136/jcp.34.9.1069-c · Cited by 2,405 · see citing works
A simple and rapid preparation of alditol acetates for monosaccharide analysis
Blakeney, A. B., Harris, P. J., Henry, R. J., & Stone, B. (1983) · Carbohydrate Research · doi:10.1016/0008-6215(83)88244-5 · Cited by 1,971 · see citing works
Manual of methods for general bacteriology.
Gerhardt, P., & Murray, R. G. E. (1981) · Medical Entomology and Zoology · Cited by 1,752 · see citing works
Dische, Z., & Shettles, L. B. (1948) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)57178-7 · Cited by 1,669 · see citing works
Hugh, R., & Leifson, E. (1953) · Journal of Bacteriology · doi:10.1128/jb.66.1.24-26.1953 · Cited by 1,665 · see citing works
Andrew, E. R., & Carr, H. Y. (1956) · Physics Today · doi:10.1063/1.3059898 · Cited by 1,192 · see citing works
Kloareg, B., & Quatrano, R. S. (1988) · Oceanography and Marine Biology/Oceanography and marine biology - an annual review · Cited by 524 · see citing works
Self-citationCarbon-13 Nuclear Magnetic Resonance Data for Oligosaccharides
Bock, K., Pedersen, C. M., & Pedersen, H. (1984) · Advances in carbohydrate chemistry and biochemistry · doi:10.1016/s0065-2318(08)60125-0 · Cited by 474 · see citing works
Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes
ZoBell, C. E. (1941) · Medical Entomology and Zoology · Cited by 428 · see citing works
Structure of a fucoidan from the brown seaweed Fucus evanescens C.Ag.
Bilan, M. I., Grachev, A. A., Ustuzhanina, N. E., Shashkov, A. S., Nifantiev, N. E., & Usov, A. I. (2002) · Carbohydrate Research · doi:10.1016/s0008-6215(02)00053-8 · Cited by 412 · see citing works
Structure and Anticoagulant Activity of Sulfated Fucans
Pereira, M. S., Mulloy, B., & Mourão, P. A. S. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.12.7656 · Cited by 377 · see citing works
Structure and Anticoagulant Activity of Sulfated Galactans
Farias, W. R. L., Valente, A. P., Pereira, M. S., & Mourão, P. A. S. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m002422200 · Cited by 369 · see citing works
Hicks, R. E., Amann, R., & Stahl, D. A. (1992) · Applied and Environmental Microbiology · doi:10.1128/aem.58.7.2158-2163.1992 · Cited by 323 · see citing works
Kane, M. D., Poulsen, L. K., & Stahl, D. A. (1993) · Applied and Environmental Microbiology · doi:10.1128/aem.59.3.682-686.1993 · Cited by 323 · see citing works
A disaccharide repeat unit is the major structure in fucoidans from two species of brown algae
Chevolot, L., Mulloy, B., Ratiskol, J., Foucault, A. P., & Colliec‐Jouault, S. (2001) · Carbohydrate Research · doi:10.1016/s0008-6215(00)00314-1 · Cited by 304 · see citing works
AUTOMATISATION DU DOSAGE DES SUBSTANCES PECTIQUES PAR LA METHODE AU METAHYDROXYDIPHENYL
Thibault, J. (1979) · Cited by 258 · see citing works
A highly regular fraction of a fucoidan from the brown seaweed Fucus distichus L.
Bilan, M. I., Grachev, A. A., Ustuzhanina, N. E., Shashkov, A. S., Nifantiev, N. E., & Usov, A. I. (2004) · Carbohydrate Research · doi:10.1016/j.carres.2003.10.028 · Cited by 255 · see citing works
Klarzynski, O., Descamps, V., Plesse, B., Yvin, J.-C., Kloareg, B., & Fritig, B. (2003) · Molecular Plant-Microbe Interactions · doi:10.1094/mpmi.2003.16.2.115 · Cited by 204 · see citing works
Antitumor Active Fucoidan from the Brown Seaweed,<i>Umitoranoo</i>(<i>Sargassum thunbergii</i>)
Zhuang, C., Itoh, H., Mizuno, T., & Ito, H. (1995) · Bioscience, Biotechnology, and Biochemistry · doi:10.1271/bbb.59.563 · Cited by 184 · see citing works
A convenient ferricyanide estimation of reducing sugars in the nanomole range
Kidby, D. K., & Davidson, D. J. (1973) · Analytical Biochemistry · doi:10.1016/0003-2697(73)90323-0 · Cited by 180 · see citing works
Bowman, J. P., McCammon, S. A., Brown, J., et al. (1997) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-47-3-670 · Cited by 177 · see citing works
Mabeau, S., & Kloareg, B. (1987) · Journal of Experimental Botany · doi:10.1093/jxb/38.9.1573 · Cited by 165 · see citing works
Self-citationBarbeyron, T., L’Haridon, S., Corre, E., Kloareg, B., & Potin, P. (2001) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/00207713-51-3-985 · Cited by 162 · see citing works
Self-citationCell Wall Is Required for Fixation of the Embryonic Axis in Fucus Zygotes
Kropf, D. L., Kloareg, B., & Quatrano, R. S. (1988) · Science · doi:10.1126/science.3336780 · Cited by 148 · see citing works
Self-citationMin, H., & Cowman, M. K. (1986) · Analytical Biochemistry · doi:10.1016/0003-2697(86)90437-9 · Cited by 146 · see citing works
Béress, A., Wassermann, O., Bruhn, T., Béress, L., Kraiselburd, E. N., Gonazalez, L. V., de Motta, G. E., & Chavez, P. I. (1993) · Journal of Natural Products · doi:10.1021/np50094a005 · Cited by 144 · see citing works
An Antivirally Active Sulfated Polysaccharide from Sargassum horneri (TURNER) C. AGARDH.
HOSHINO, T., Hayashi, T., Hayashi, K., Hamada, J., Lee, J., & Sankawa, U. (1998) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.21.730 · Cited by 131 · see citing works
Fractionation and analysis of fucans from brown algae
Mabeau, S., Kloareg, B., & Joseleau, J. P. (1990) · Phytochemistry · doi:10.1016/0031-9422(90)85163-a · Cited by 128 · see citing works
Self-citationLipkind, G. M., Shashkov, A. S., Mamyan, S. S., et al. (1988) · Carbohydrate Research · doi:10.1016/0008-6215(88)84018-7 · Cited by 128 · see citing works
Fucus Embryogenesis: A Model to Study the Establishment of Polarity.
Goodner, B. W., & Quatrano, R. S. (1993) · The Plant Cell · doi:10.1105/tpc.5.10.1471 · Cited by 125 · see citing works
Riou, D., Colliec‐Jouault, S., du Sel, D. P., Bösch, S., Siavoshian, S., Bert, V. L., Tomasoni, C., Sinquin, C., Durand, P., & Roussakis, C. (1996) · PubMed · Cited by 124 · see citing works
Ley, K. F., Linnemann, G., Meinen, M., Stoolman, L., & Gaehtgens, P. A. L. (1993) · Blood · doi:10.1182/blood.v81.1.177.177 · Cited by 124 · see citing works
Vilela-Silva, A.-C. E. S., Castro, M. O., Valente, A. P., Biermann, C. H., & Mourão, P. A. S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m108496200 · Cited by 116 · see citing works
Bock, K., & Thoegersen, H. (1984) · ChemInform · doi:10.1002/chin.198410334 · Cited by 104 · see citing works
Colliec‐Jouault, S., Mauray, S., Theveniaux, J., Sternberg, C., Vidal, B., Fischer, A., & Millet, J. (1999) · Thrombosis and Haemostasis · doi:10.1055/s-0037-1614484 · Cited by 95 · see citing works
Daniel, R., Berteau, O., Jozefonvicz, J., & Goasdoué, N. (1999) · Carbohydrate Research · doi:10.1016/s0008-6215(99)00223-2 · Cited by 84 · see citing works
Berteau, O., McCort‐Tranchepain, I., Goasdoué, N., Tissot, B., & Daniel, R. (2002) · Glycobiology · doi:10.1093/glycob/12.4.273 · Cited by 84 · see citing works
Shimanaka, K., Ikai, K., Kato, I., Sakai, T., & Ishizuka, K. (2003) · Marine Biotechnology · doi:10.1007/s10126-002-0107-9 · Cited by 83 · see citing works
The pigments of Flexibacter elegans: Novel and chemosystematically useful compounds
Reichenbach, H., Kleinig, H., & Achenbach, H. (1974) · Archives of Microbiology · doi:10.1007/bf00455933 · Cited by 83 · see citing works
Isolation and Characterization of a Fucoidan-Degrading Marine Bacterium
Sakai, T., Ishizuka, K., & Kato, I. (2003) · Marine Biotechnology · doi:10.1007/s10126-002-0118-6 · Cited by 81 · see citing works
A Marine Strain of Flavobacteriaceae Utilizes Brown Seaweed Fucoidan
Sakai, T., Kimura, H., & Kato, I. (2002) · Marine Biotechnology · doi:10.1007/s10126-002-0032-y · Cited by 75 · see citing works
Thorlacius, Vollmar, Seyfert, Vestweber, & Menger (2000) · European Journal of Clinical Investigation · doi:10.1046/j.1365-2362.2000.00704.x · Cited by 64 · see citing works
Fucoidin inhibits the zona pellucida-induced acrosome reaction in human spermatozoa
Mahony, M. C., Oehninger, S., Clark, G. F., Acosta, A. A., & Hodgen, G. D. (1991) · Contraception · doi:10.1016/0010-7824(91)90085-t · Cited by 63 · see citing works
Sakai, T., Kimura, H., Kojima, K., SHIMANAKA, K., IKAI, K., & Kato, I. (2003) · Marine Biotechnology · doi:10.1007/s10126-002-0056-3 · Cited by 59 · see citing works
Østergaard, C., Yieng-Kow, R. V., Benfield, T., Møller, J. K., Espersen, F., & Lundgren, J. D. (2000) · Infection and Immunity · doi:10.1128/iai.68.6.3153-3157.2000 · Cited by 59 · see citing works
Kitamura, K., Matsuo, M., & Tsuneo, Y. (1992) · Bioscience, Biotechnology, and Biochemistry · doi:10.1271/bbb.56.490 · Cited by 56 · see citing works
Logeart‐Avramoglou, D., Prigent-Richard, S., Jozefonvicz, J., et al. (1997) · PubMed · Cited by 56 · see citing works
Fucoidan is a non-anticoagulant inhibitor of intimal hyperplasia
McCaffrey, T. A., Falcone, D. J., Borth, W., et al. (1992) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(92)90657-7 · Cited by 46 · see citing works
Enzymic degradation of fucoidan by enzymes from the hepatopancreas of abalone, Haliotus species
Thanassi, N. M., & Nakada, H. I. (1967) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(67)90294-9 · Cited by 43 · see citing works
A Partially Pyruvated Carrageenan from Hawaiian Grateloupia filicina (Cryptonemiales, Rhodophyta)
Zablackis, E., & Perez, J. (1990) · Botanica Marina · doi:10.1515/botm.1990.33.3.273 · Cited by 42 · see citing works
Method Designed To Detect Alginate-Degrading Bacteria
Kitamikado, M., Yamaguchi, K., Tseng, C.-H., et al. (1990) · Applied and Environmental Microbiology · doi:10.1128/aem.56.9.2939-2940.1990 · Cited by 36 · see citing works
Riou, D., Colliec‐Jouault, S., du Sel, D. P., Bösch, S., Siavoshian, S., Bert, V. L., Tomasoni, C., Sinquin, C., Durand, P., & Roussakis, C. (1996) · Cited by 33 · see citing works
Dalton, J. P., Hudson, D. E., Adams, J. H., et al. (1991) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1991.tb15767.x · Cited by 28 · see citing works
Production of Fucoidan-Degrading Enzymes, Fucoidanase, and Fucoidan Sulfatase by Vibrio sp. N-5.
Furukawa, S.-I., Fujikawa, T., Koga, D., & Ide, A. (1992) · NIPPON SUISAN GAKKAISHI · doi:10.2331/suisan.58.1499 · Cited by 24 · see citing works
Fucoidin binding activity and its localization on human spermatozoa
Mahony, M. C., Clark, G. F., Oehninger, S., Acosta, A. A., & Hodgen, G. D. (1993) · Contraception · doi:10.1016/0010-7824(93)90146-x · Cited by 22 · see citing works
The effects of inhibiting leukocyte migration with fucoidin in a rat peritonitis model
Linnemann, G., Reinhart, K., Parade, U., et al. (2000) · Intensive Care Medicine · doi:10.1007/s001340000642 · Cited by 20 · see citing works
Studies on by-products from the industrial extraction of alginate
Fleury, N., & Lahaye, M. (1993) · Journal of Applied Phycology · doi:10.1007/bf02184639 · Cited by 19 · see citing works
Self-citationWikström, T., Braide, M., BAGGE, U., et al. (1995) · Shock · doi:10.1097/00024382-199501000-00006 · Cited by 16 · see citing works
Uchida, M. (1995) · Marine Biology · doi:10.1007/bf00349242 · Cited by 12 · see citing works
Mayer, A. M. S., Diaz, A., Pesce, A., et al. (1987) · Hydrobiologia · doi:10.1007/bf00046173 · Cited by 8 · see citing works
Mayer, A. M. S., Diaz, A., Pesce, A., Criscuolo, M. E., Groisman, J. F., & de Lederkremer, R. M. (1987) · Springer eBooks · doi:10.1007/978-94-009-4057-4_73 · Cited by 7 · see citing works
A search for fucoidan-degrading bacteria in coastal sea environments of Japan.
Morinaga, T., Araki, T., Ito, M., & Kitamikado, M. (1981) · NIPPON SUISAN GAKKAISHI · doi:10.2331/suisan.47.621 · Cited by 7 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Ale, M. T., & Meyer, A. S. (2013) · RSC Adv. · doi:10.1039/c3ra23373a · Cited by 381 · see citing works
Novel procedures for the extraction of fucoidan from brown algae
Hahn, T., Lang, S., Ulber, R., & Muffler, K. (2012) · Process Biochemistry · doi:10.1016/j.procbio.2012.06.016 · Cited by 297 · see citing works
Structure, biological activity, and enzymatic transformation of fucoidans from the brown seaweeds
Kusaykin, M., Bakunina, I., Sova, V., Ermakova, S., Kuznetsova, T., Besednova, N., Zaporozhets, T., & Zvyagintseva, T. (2008) · Biotechnology Journal · doi:10.1002/biot.200700054 · Cited by 197 · see citing works
Holtkamp, A. D., Kelly, S., Ulber, R., & Lang, S. (2009) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-008-1790-x · Cited by 175 · see citing works
Dye affinity chromatography for fast and simple purification of fucoidan from marine brown algae
Hahn, T., Zayed, A., Kovacheva, M., Stadtmüller, R., Lang, S., Muffler, K., & Ulber, R. (2016) · Engineering in Life Sciences · doi:10.1002/elsc.201500044 · Cited by 40 · see citing works
- Authors
- Descamps, V., Colin, S., Lahaye, M., Jam, M., Richard, C., Potin, P., Barbeyron, T., Yvin, J.-C., & Kloareg, B.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Institut National de la Recherche Agronomique (MA)
- Corresponding author
- Barbeyron, Tristan
- ORCID
- Venue
- Marine Biotechnology
- Published
- 2006-01
- Volume
- 8
- Issue
- 1
- Pages
- 27-39
- ISSN
- 1436-2228 (ISSN-L)
- 1436-2236
- PMC
- PMC4273248
- OpenAlex
- W2088256689
- Cited by
- 108
- References
- 65
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 3 · 2023: 4 · 2022: 7 · 2021: 9 · 2020: 6 · 2019: 5 · 2018: 6 · 2017: 9 · 2016: 8 · 2015: 10 · 2014: 7 · 2013: 6 · 2012: 6
- FWCI
- 3.01
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Marine Biotechnology (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Association Nationale de la Recherche et de la Technologie
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- fucoidanase, sulfated fucans, marine brown algae, Flavobacteriaceae, Pelvetia canaliculata, extracellular enzymes, polysaccharide degradation, bacterial isolation, culture conditions
- MeSH terms
- Phaeophyceae, Biodegradation, Environmental, Chromatography, Hydrolases, Magnetic Resonance Imaging, Oligosaccharides, Phylogeny, Polysaccharides, Geologic Sediments, Flavobacteriaceae
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
