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Cited by 70Per- and polyfluoroalkyl substances researchEnvironmental Toxicology and EcotoxicologyZebrafish Biomedical Research Applications

Chronic PFOS Exposures Induce Life Stage-specific Behavioral Deficits in Adult Zebrafish and Produce Malformation and Behavioral Deficits in F1 Offspring

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Chen, Jiangfei, Das, Siba R., La Du, Jane, Corvi, Margaret M., Bai, Chenglian, Chen, Yuanhong, Liu, Xiaojuan, Zhu, Guonian +3 more·2013·Environmental Toxicology and Chemistry
DOI 10.1002/etc.2031PMC PMC4049192
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Abstract

Perfluorooctane sulfonic acid (PFOS) is an organic contaminant that is ubiquitous in the environment. Few studies have assessed the behavioral effects of chronic PFOS exposure in aquatic organisms. The present study defined the behavioral effects of varying life span chronic exposures to PFOS in zebrafish. Specifically, zebrafish were exposed to control or 0.5 µM PFOS during 1 to 20, 21 to 120, or 1 to 120 d postfertilization (dpf). Exposure to PFOS impaired the adult zebrafish behavior mode under the tapping stimulus. The movement speed of male and female fish exposed for 1 to 120 dpf was significantly increased compared with control before and after tapping, whereas in the groups exposed for 1 to 20 and 21 to 120 dpf, only the males exhibited elevated swim speed before tapping. Residues of PFOS in F1 embryos derived from parental exposure for 1 to 120 and 21 to 120 dpf were significantly higher than control, and F1 embryos in these two groups also showed high malformation and mortality. The F1 larvae of parental fish exposed to PFOS for 1 to 20 or 21 to 120 dpf exhibited a higher swimming speed than control larvae in a light-to-dark behavior assessment test. The F1 larvae derived from parental fish exposed to PFOS for 1 to 120 dpf showed a significantly lower speed in the light period and a higher speed in the dark period compared with controls. Although there was little PFOS residue in embryos derived from the 1- to 20-dpf parental PFOS-exposed group, the adverse behavioral effects on both adult and F1 larvae indicate that exposure during the first 21 dpf induces long-term neurobehaviorial toxicity. The authors' findings demonstrate that chronic PFOS exposure during different life stages adversely affects adult behavior and F1 offspring morphology, behavior, and survival.

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Crossref · 10.1002/etc.2031

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  • In zebrafish, F1 progeny with no direct PFOS exposure, born to parents embryonically exposed to PFOS, experienced behavioral deficits attributed to maternal transfer of PFOS to eggs.

    “In studies reporting exposures in zebrafish, F1 progeny with no direct PFOS exposure, born to parents embryonically exposed to PFOS, also experienced behavioral deficits, which were attributed to maternal transfer of PFOS to eggs (Wang M. et al., 2011; Chen et al., 2013).”

    From A Critical Review and Meta-Analysis of Impacts of Per- and Polyfluorinated Substances on the Brain and Behavior (2022) · cites this paper as Chen et al., 2013

    The Methods (§2.5) say F1 larvae developed without PFOS added to their environment, and the Results (§3.4) report altered behavior in offspring of parents exposed during days 1–21. But the Discussion attributes possible maternal transfer to malformations in other exposure groups, not to the behavioral deficits in the 1–21-day group; it says the reason for those behavioral changes needs further investigation. Page numbers were not available in our copy. (LLM, conf=0.90)

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  • While concentrations of PFAS measured in adult tissues were often low following developmental exposure, those exposures still caused neurobehavioral toxicity persisting into adulthood.

    “While concentrations of PFAS measured in adult tissues were often low following developmental exposure, those exposures still caused neurobehavioral toxicity persisting into adulthood (Johansson et al., 2008; Chen et al., 2013).”

    From A Critical Review and Meta-Analysis of Impacts of Per- and Polyfluorinated Substances on the Brain and Behavior (2022) · cites this paper as Chen et al., 2013

    The Discussion reports that zebrafish exposed to PFOS during days 1–21 had little PFOS residue yet showed behavioral effects as adults. However, the Methods (§2.4) specify that residues were measured in F1 embryos, not adult tissues; the study also concerns PFOS specifically, rather than PFAS exposures generally. (LLM, conf=0.86)

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  • Several studies assessing PFOS exposure in larval zebrafish reported increased swimming speed or hyperactivity in PFOS-exposed adults.

    “Several studies assessing PFOS exposure in larval zebrafish reported increased swimming speed or hyperactivity in PFOS-exposed adults (Huang et al., 2010; Chen et al., 2013; Gaballah et al., 2020).”

    From A Critical Review and Meta-Analysis of Impacts of Per- and Polyfluorinated Substances on the Brain and Behavior (2022) · cites this paper as Chen et al., 2013

    The Introduction and Methods (§2.2–2.3; page unavailable) show that this study exposed zebrafish beginning in the larval stage and assessed their swimming as adults. But the claim glosses over the exposure window: the reported increase in adult movement speed was for fish exposed from 1–120 days post-fertilization, not specifically the larval-only group. The higher swim rates in Results §3.4 (page unavailable) concern F1 larvae, not the exposed adults. (LLM, conf=0.78)

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Research works printed but not linked: 2

  1. Butenhoff JL Ehresman DJ Chang SC Parker GA Stump DG 2009b Gestational and lactational exposure to potassium perfluorooctanesulfonate (K+PFOS) in rats: developmental neurotoxicity Reprod Toxicol 27 3–4 319 30 19162172
  2. Nishikawa Y Sato I Tsuda S 2004 Detection of Genotoxicity and Neurotoxicity of Perfluorooctane Sulfonate (PFOS) and Perfluorooctanoic (PFOA) in Paramecia Proceedings of the 138th Congress of Japanese Society of Veterinary Science 198

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Authors
Chen, J., Das, S. R., La Du, J., Corvi, M. M., Bai, C., Chen, Y., Liu, X., Zhu, G., Tanguay, R. L., Dong, Q., & Huang, C.
Affiliations
  • Wenzhou Medical University (CN)
  • Oregon State University (US)
  • Oregon State University, Corvallis, Oregon, USA
  • Zhejiang University (CN)
Corresponding author
Huang, Changjiang
ORCID
Venue
Environmental Toxicology and Chemistry
Publisher
Oxford University Press (OUP)
Published
2012-10-11
Volume
32
Issue
1
Pages
201-206
ISSN
  • 0730-7268 (ISSN-L)
  • 1552-8618
DOI
10.1002/etc.2031
PMC
PMC4049192
OpenAlex
W1972541502
Cited by
70
References
35
Citations by year
2026: 6 · 2025: 7 · 2024: 5 · 2023: 7 · 2022: 6 · 2021: 3 · 2020: 3 · 2019: 8 · 2018: 4 · 2017: 7 · 2016: 6 · 2015: 1 · 2014: 5 · 2013: 2
FWCI
2.05
Type
journal article
Language
en
Open access
Licence
Locations
  • Environmental Toxicology and Chemistry (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of Environmental Health Sciences (P30ES000210)
Topics
  • Physical Sciences › Environmental Science › Environmental Chemistry › Per- and polyfluoroalkyl substances research
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
Keywords
PFOS, long-term exposure, zebrafish, developmental toxicity, neurobehavioral toxicity, swimming speed, behavioral deficits, embryonic malformations, F1 offspring, transgenerational effects, life stage sensitivity
MeSH terms
Animals, Behavior, Animal, Female, Fluorocarbons, Larva, Male, Water Pollutants, Chemical, Zebrafish, Alkanesulfonic Acids, Toxicity Tests, Chronic

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