1H NMR‐based metabolomics reveal overlapping discriminatory metabolites and metabolic pathway disturbances between colorectal tumor tissues and fecal samples
Open accessAbstract
Previous studies have compared fecal metabolites from healthy and colorectal cancer (CRC) patients to predict the pro‐CRC signatures. However, the systemic mechanistic link between feces and colonic tissues of CRC patients is still limited. The current study was a paralleled investigation of colonic tumor tissues and their normal adjacent tissues alongside patient‐matched feces by using 1H nuclear magnetic resonance spectroscopy combined with pattern recognition to investigate how fecal metabolic phenotypes are linked to the changes in colorectal tumor profiles. A set of overlapping discriminatory metabolites across feces and tumor tissues of CRC were identified, including elevated levels of lactate, glutamate, alanine, succinate and reduced amounts of butyrate. These changes could indicate the networks for metabolic pathway perturbations in CRC potentially involved in the disruptions of glucose and glycolytic metabolism, TCA cycle, glutaminolysis, and short chain fatty acids metabolism. Furthermore, changes in fecal acetate were positively correlated with alterations of glucose and myo‐inositol in colorectal tumor tissues, implying enhanced energy production for rapid cell proliferation. Compared to other fecal metabolites, acetate demonstrated the highest diagnostic performance for diagnosing CRC, with an AUC of 0.843 in the training set, and a good predictive ability in the validation set. Overall, these associations provide evidence of distinct metabolic signatures and metabolic pathway disturbances between the colonic tissues and feces within the same individual, and changes of fecal metabolic signature could reflect the CRC tissue microenvironment, highlighting the significance of the distinct fecal metabolic profiles as potential novel and noninvasive relevant indicators for CRC detection.
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A few cross-sectional studies reported that colorectal cancer patients had a lower abundance of butyrate-producing species and lower fecal butyrate levels than controls.
“a few cross-sectional studies reported that patients with CRC had a lower abundance of butyrate-producing species and lower fecal levels of butyrate than controls (Table 1).97, 133–135”
From Influence of the Gut Microbiome, Diet, and Environment on Risk of Colorectal Cancer (2020) · cites this paper as [133]
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Cited by
Sorted by citations
Influence of the Gut Microbiome, Diet, and Environment on Risk of Colorectal Cancer
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- Authors
- Lin, Y., Ma, C., Bezabeh, T., Wang, Z., Liang, J., Huang, Y., Zhao, J., Liu, X., Ye, W., Tang, W., Ouyang, T., & Wu, R.
- Affiliations
- Shantou University (CN)
- Cancer Hospital of Shantou University Medical College (CN)
- University of Guam (GU)
- Department of Radiology Second Affiliated Hospital, Shantou University Medical College Shantou Guangdong Province China
- Corresponding author
- Lin, Yan
- Wu, Renhua
- Venue
- International Journal of Cancer
- Publisher
- Wiley
- Published
- 2019-03-04
- Volume
- 145
- Issue
- 6
- Pages
- 1679-1689
- ISSN
- 0020-7136 (ISSN-L)
- 1097-0215
- OpenAlex
- W2911842670
- Cited by
- 57
- References
- 38
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 10 · 2023: 9 · 2022: 8 · 2021: 12 · 2020: 6 · 2019: 2
- FWCI
- 2.32
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- International Journal of Cancer (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81471729, 81101102)
- Department of Education of Guangdong Province (2017KTSCX071)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Physical Sciences › Engineering › Biomedical Engineering › Advanced Chemical Sensor Technologies
- Keywords
- colorectal cancer, fecal metabolites, tumor microenvironment, short-chain fatty acids, butyrate, lactate, glutamine metabolism, TCA cycle, glycolysis, myo-inositol, non-invasive cancer diagnosis, diagnostic biomarkers
- MeSH terms
- Feces, Female, Humans, Male, Middle Aged, Biomarkers, Tumor, Colorectal Neoplasms, Case-Control Studies, Early Detection of Cancer, Metabolomics, Proton Magnetic Resonance Spectroscopy
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