An RNA-seq protocol to identify mRNA expression changes in mouse diaphyseal bone: Applications in mice with bone property altering Lrp5 mutations
Open accessAbstract
Loss-of-function and certain missense mutations in the Wnt coreceptor low-density lipoprotein receptor-related protein 5 (LRP5) significantly decrease or increase bone mass, respectively. These human skeletal phenotypes have been recapitulated in mice harboring Lrp5 knockout and knock-in mutations. We hypothesized that measuring mRNA expression in diaphyseal bone from mice with Lrp5 wild-type (Lrp5+/+), knockout (Lrp5–/–), and high bone mass (HBM)-causing (Lrp5p.A214V/+) knock-in alleles could identify genes and pathways that regulate or are regulated by LRP5 activity. We performed RNA-seq on pairs of tibial diaphyseal bones from four 16-week-old mice with each of the aforementioned genotypes. We then evaluated different methods for controlling for contaminating nonskeletal tissue (ie, blood, bone marrow, and skeletal muscle) in our data. These methods included predigestion of diaphyseal bone with collagenase and separate transcriptional profiling of blood, skeletal muscle, and bone marrow. We found that collagenase digestion reduced contamination, but also altered gene expression in the remaining cells. In contrast, in silico filtering of the diaphyseal bone RNA-seq data for highly expressed blood, skeletal muscle, and bone marrow transcripts significantly increased the correlation between RNA-seq data from an animal's right and left tibias and from animals with the same Lrp5 genotype. We conclude that reliable and reproducible RNA-seq data can be obtained from mouse diaphyseal bone and that lack of LRP5 has a more pronounced effect on gene expression than the HBM-causing LRP5 missense mutation. We identified 84 differentially expressed protein-coding transcripts between LRP5 “sufficient” (ie, Lrp5+/+ and Lrp5p.A214V/+) and “insufficient” (Lrp5–/–) diaphyseal bone, and far fewer differentially expressed genes between Lrp5p.A214V/+ and Lrp5+/+ diaphyseal bone. © 2013 American Society for Bone and Mineral Research.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
538 genes enriched in bone marrow or contaminating tissues were excluded, leaving 1239 genes significantly enriched for expression in osteocytes.
“As an additional level of stringency 538 genes enriched in bone marrow or tissues that could contaminate the osteocyte enrichment strategy, such as blood or muscle (Fig. 3c and Supplementary Data 2)13, were excluded leaving 1239 genes significantly enriched for expression in osteocytes”
From Osteocyte transcriptome mapping identifies a molecular landscape controlling skeletal homeostasis and susceptibility to skeletal disease (2021) · cites this paper as [13]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Controlling the False Discovery Rate: A Practical and Powerful Approach to Multiple Testing
Benjamini, Y., & Hochberg, Y. (1995) · Journal of the Royal Statistical Society Series B: Statistical Methodology · doi:10.1111/j.2517-6161.1995.tb02031.x · Cited by 109,482 · see citing works
The Sequence Alignment/Map format and SAMtools
Li, H., Handsaker, B., Wysoker, A., Fennell, T., Ruan, J., Homer, N., Marth, G., Abecasis, G., Durbin, R., & 1000 Genome Project Data Processing Subgroup (2009) · Bioinformatics · doi:10.1093/bioinformatics/btp352 · Cited by 62,041 · see citing works
Robinson, M. D., McCarthy, D. J., & Smyth, G. K. (2010) · Bioinformatics · doi:10.1093/bioinformatics/btp616 · Cited by 41,364 · see citing works
Mapping and quantifying mammalian transcriptomes by RNA-Seq
Mortazavi, A., Williams, B. A., McCue, K., Schaeffer, L., & Wold, B. (2008) · Nature Methods · doi:10.1038/nmeth.1226 · Cited by 13,696 · see citing works
Accurate whole human genome sequencing using reversible terminator chemistry
Bentley, D. R., Balasubramanian, S., Swerdlow, H. P., Smith, G. P., Milton, J., Brown, C. G., Hall, K. P., Evers, D. J., Barnes, C. L., Bignell, H. R., et al. (2008) · Nature · doi:10.1038/nature07517 · Cited by 3,684 · see citing works
The RIN: an RNA integrity number for assigning integrity values to RNA measurements
Schroeder, A., Mueller, O., Stocker, S., Salowsky, R., Leiber, M., Gassmann, M., Lightfoot, S., Menzel, W., Granzow, M., & Ragg, T. (2006) · BMC Molecular Biology · doi:10.1186/1471-2199-7-3 · Cited by 2,623 · see citing works
The Transcriptional Landscape of the Yeast Genome Defined by RNA Sequencing
Nagalakshmi, U., Wang, Z., Waern, K., Shou, C., Raha, D., Gerstein, M., & Snyder, M. (2008) · Science · doi:10.1126/science.1158441 · Cited by 2,479 · see citing works
LDL Receptor-Related Protein 5 (LRP5) Affects Bone Accrual and Eye Development
Gong, Y., Slee, R. B., Fukai, N., Rawadi, G., Roman-Roman, S., Reginato, A. M., Wang, H., Cundy, T., Glorieux, F. H., Lev, D., et al. (2001) · Cell · doi:10.1016/s0092-8674(01)00571-2 · Cited by 2,235 · see citing works
Self-citationRNA sequencing: advances, challenges and opportunities
Ozsolak, F., & Milos, P. M. (2011) · Nature Reviews Genetics · doi:10.1038/nrg2934 · Cited by 2,135 · cited 2× · see citing works
“Massively parallel sequencing (also referred to as next generation sequencing) of mRNA (RNA‐seq) has quickly become a powerful alternative to measuring mRNA expression by microarray and quantitative RT‐PCR.(16,17) The advantages of RNA‐seq are that it can cover a broad range of transcript abundance and can identify mR…”
BackgroundDirect multiplexed measurement of gene expression with color-coded probe pairs
Geiss, G. K., Bumgarner, R. E., Birditt, B., Dahl, T., Dowidar, N., Dunaway, D. L., Fell, H. P., Ferree, S., George, R. D., Grogan, T., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1385 · Cited by 2,066 · see citing works
High Bone Density Due to a Mutation in LDL-Receptor–Related Protein 5
Boyden, L. M., Mao, J., Belsky, J., Mitzner, L., Farhi, A., Mitnick, M. A., Wu, D., Insogna, K., & Lifton, R. P. (2002) · New England Journal of Medicine · doi:10.1056/nejmoa013444 · Cited by 1,591 · see citing works
Sclerostin Binds to LRP5/6 and Antagonizes Canonical Wnt Signaling
Li, X., Zhang, Y., Kang, H., Liu, W., Liu, P., Zhang, J., Harris, S. E., & Wu, D. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m413274200 · Cited by 1,366 · cited 1× · see citing works
“Interestingly, we detected no significant changes in the expression levels of other Wnt pathway components, including Sost, Dkk1, Lrp4, and Lrp6, each of which can significantly affect bone mass in humans and/or mice.((10,37,38)) Lrp5 also serves as an important positive control for the RNA‐ seq data involving Lrp5p.”
BackgroundThe molecular understanding of osteoclast differentiation
Asagiri, M., & Takayanagi, H. (2007) · Bone · doi:10.1016/j.bone.2006.09.023 · Cited by 1,363 · see citing works
Matrix-embedded cells control osteoclast formation
Xiong, J., Onal, M., Jilka, R. L., Weinstein, R. S., Manolagas, S. C., & O'Brien, C. A. (2011) · Nature Medicine · doi:10.1038/nm.2448 · Cited by 1,304 · see citing works
Little, R. D., Folz, C., Manning, S. P., Swain, P. M., Zhao, S.-C., Eustace, B., Lappe, M. M., Spitzer, L., Zweier, S., Braunschweiger, K., et al. (2002) · The American Journal of Human Genetics · doi:10.1086/338450 · Cited by 1,282 · see citing works
Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries
Aird, D., Ross, M. G., Chen, W.-S., Danielsson, M., Fennell, T., Russ, C., Jaffe, D. B., Nusbaum, C., & Gnirke, A. (2011) · Genome Biology · doi:10.1186/gb-2011-12-2-r18 · Cited by 1,174 · see citing works
IL-6 is produced by osteoblasts and induces bone resorption.
Ishimi, Y., Miyaura, C., Jin, C., Akatsu, T., Abe, E., Nakamura, Y. K., Yamaguchi, A., Yoshiki, S., Matsuda, T., Hirano, T., et al. (1990) · The Journal of Immunology · doi:10.4049/jimmunol.145.10.3297 · Cited by 1,128 · see citing works
Substantial biases in ultra-short read data sets from high-throughput DNA sequencing
Dohm, J. C., Lottaz, C., Borodina, T., & Himmelbauer, H. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkn425 · Cited by 1,088 · see citing works
Novel mechanism of Wnt signalling inhibition mediated by Dickkopf-1 interaction with LRP6/Arrow
Bafico, A., Liu, G., Yaniv, A., Gazit, A., & Aaronson, S. A. (2001) · Nature Cell Biology · doi:10.1038/35083081 · Cited by 772 · see citing works
SOST Is a Ligand for LRP5/LRP6 and a Wnt Signaling Inhibitor
Semënov, M., Tamai, K., & He, X. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m504308200 · Cited by 771 · see citing works
Head inducer Dickkopf-1 is a ligand for Wnt coreceptor LRP6
Semënov, M. V., Tamai, K., Brott, B. K., Kühl, M., Sokol, S. Y., & He, X. (2001) · Current Biology · doi:10.1016/s0960-9822(01)00290-1 · Cited by 700 · see citing works
The human complement factor H: functional roles, genetic variations and disease associations
de Córdoba, S. R., Esparza-Gordillo, J., GOICOECHEADEJORGE, E., López‐Trascasa, M., & Sánchez‐Corral, P. (2004) · Molecular Immunology · doi:10.1016/j.molimm.2004.02.005 · Cited by 572 · see citing works
Van Wesenbeeck, L., Cleiren, E., Gram, J., Beals, R. K., Bénichou, O., Scopelliti, D., Key, L., Renton, T., Bartels, C., Gong, Y., et al. (2003) · The American Journal of Human Genetics · doi:10.1086/368277 · Cited by 568 · see citing works
Self-citationKozarewa, I., Ning, Z., Quail, M. A., Sanders, M. J., Berriman, M., & Turner, D. J. (2009) · Nature Methods · doi:10.1038/nmeth.1311 · Cited by 565 · see citing works
Microarrays, deep sequencing and the true measure of the transcriptome
Malone, J. H., & Oliver, B. (2011) · BMC Biology · doi:10.1186/1741-7007-9-34 · Cited by 546 · see citing works
Wnt/β-Catenin Signaling Is a Normal Physiological Response to Mechanical Loading in Bone
Robinson, J. A., Chatterjee‐Kishore, M., Yaworsky, P. J., Cullen, D. M., Zhao, W., Li, C., Kharode, Y. P., Sauter, L., Babij, P., Brown, E. L., et al. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m602308200 · Cited by 522 · see citing works
Clément-Lacroix, P., Ai, M., Morvan, F., Roman‐Roman, S., Vayssière, B., Belleville, C., Estrera, K. A., Warman, M. L., Baron, R., & Rawadi, G. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0505259102 · Cited by 520 · see citing works
Self-citationLrp5 functions in bone to regulate bone mass
Cui, Y., Niziolek, P., MacDonald, B. T., Diegel, C. R., Alenina, N. V., Robinson, D. R., Zhong, Z. A., Matthes, S., Jacobsen, C. M., Conlon, R. A., et al. (2011) · Nature Medicine · doi:10.1038/nm.2388 · Cited by 454 · see citing works
Self-citationSawakami, K., Robling, A. G., Ai, M., Pitner, N. D., Liu, D., Warden, S. J., Li, J., Maye, P. F., Rowe, D. W., Duncan, R. L., et al. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m601000200 · Cited by 397 · see citing works
Self-citationDecreased BMD and Limb Deformities in Mice Carrying Mutations in Both Lrp5 and Lrp6
Holmen, S. L., Giambernardi, T. A., Diegel, C. R., Buckner-Berghuis, B. D., Resau, J. H., Hess, J. F., Glatt, V., Bouxsein, M., Ai, M., Warman, M. L., et al. (2004) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.040907 · Cited by 344 · see citing works
Self-citationComparative analysis of RNA-Seq alignment algorithms and the RNA-Seq unified mapper (RUM)
Grant, G. R., Farkas, M. H., Pizarro, A., Lahens, N. F., Schug, J., Brunk, B. P., Stoeckert, C. J., Hogenesch, J. B., & Pierce, E. A. (2011) · Bioinformatics · doi:10.1093/bioinformatics/btr427 · Cited by 336 · see citing works
Si, W., Kang, Q., Luu, H. H., Park, J. K., Luo, Q., Song, W.-X., Jiang, W., Luo, X., Li, X., Yin, H., et al. (2006) · Molecular and Cellular Biology · doi:10.1128/mcb.26.8.2955-2964.2006 · Cited by 272 · see citing works
Ai, M., Holmen, S. L., Hul, W. V., Williams, B. O., & Warman, M. L. (2005) · Molecular and Cellular Biology · doi:10.1128/mcb.25.12.4946-4955.2005 · Cited by 253 · see citing works
Self-citationLRP5 Mutations Linked to High Bone Mass Diseases Cause Reduced LRP5 Binding and Inhibition by SOST
Semënov, M. V., & He, X. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m609509200 · Cited by 222 · see citing works
Choi, H. Y., Dieckmann, M., Herz, J., & Niemeier, A. (2009) · PLoS ONE · doi:10.1371/journal.pone.0007930 · Cited by 206 · see citing works
Srivastava, S., Weitzmann, M. N., Kimble, R. B., Rizzo, M., Zahner, M., Milbrandt, J., Ross, F. P., & Pacifici, R. (1998) · Journal of Clinical Investigation · doi:10.1172/jci4561 · Cited by 197 · see citing works
Mdmx as an essential regulator of p53 activity
Marine, J., & Jochemsen, A. G. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.03.151 · Cited by 186 · see citing works
Wnt Antagonists Bind through a Short Peptide to the First β-Propeller Domain of LRP5/6
Bourhis, E., Wang, W., Tam, C., Hwang, J., Zhang, Y., Spittler, D., Huang, O. W., Gong, Y., Estevez, A., Zilberleyb, I., et al. (2011) · Structure · doi:10.1016/j.str.2011.07.005 · Cited by 169 · see citing works
Regulation of Type I Collagen Genes Expression
Karsenty, G., & Park, R. (1995) · International Reviews of Immunology · doi:10.3109/08830189509056711 · Cited by 160 · see citing works
Baud’huin, M., Renault, R., Charrier, C., Riet, A., Moreau, A., Brion, R., Gouin, F., Duplomb, L., & Heymann, D. (2010) · The Journal of Pathology · doi:10.1002/path.2684 · Cited by 159 · see citing works
Regulation of the innate immune response by threonine-phosphatase of Eyes absent
Okabe, Y., Sano, T., & Nagata, S. (2009) · Nature · doi:10.1038/nature08138 · Cited by 154 · see citing works
Interleukin 34 expression is associated with synovitis severity in rheumatoid arthritis patients
Chemel, M., Goff, B. L., Brion, R., Cozic, C., Berreur, M., Amiaud, J., Bougras-Cartron, G., Touchais, S., Blanchard, F., Heymann, M., et al. (2011) · Annals of the Rheumatic Diseases · doi:10.1136/annrheumdis-2011-200096 · Cited by 144 · see citing works
Mcmahon, A. P., Champion, J. E., Mcmahon, J. A., & Sukhatme, V. P. (1990) · Development · doi:10.1242/dev.108.2.281 · Cited by 141 · see citing works
Bone development and inflammatory disease is regulated by AP-1 (Fos/Jun)
Wagner, E. F. (2009) · Annals of the Rheumatic Diseases · doi:10.1136/ard.2009.119396 · Cited by 140 · see citing works
Kawai, T., Nomura, F., Hoshino, K., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., & Akira, S. (1999) · Oncogene · doi:10.1038/sj.onc.1202701 · Cited by 124 · see citing works
Neuropeptide Y is expressed by osteocytes and can inhibit osteoblastic activity
Igwe, J. C., Jiang, X., Paić, F., Ma, L., Adams, D. J., Baldock, P. A. J., Pilbeam, C. C., & Kalajzić, I. (2009) · Journal of Cellular Biochemistry · doi:10.1002/jcb.22294 · Cited by 122 · see citing works
Novel Regulators of Fgf23 Expression and Mineralization in Hyp Bone
Liu, S., Tang, W., Fang, J., Ren, J., Li, H., Xiao, Z., & Quarles, L. D. (2009) · Molecular Endocrinology · doi:10.1210/me.2009-0085 · Cited by 122 · see citing works
Erythropoietin mediated bone formation is regulated by mTOR signaling
Kim, J., Jung, Y., Sun, H., Joseph, J., Mishra, A., Shiozawa, Y., Wang, J., Krebsbach, P. H., & Taichman, R. S. (2011) · Journal of Cellular Biochemistry · doi:10.1002/jcb.23347 · Cited by 115 · see citing works
Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4
Knowles, H. J., Cleton-Jansen, A., Korsching, E., & Athanasou, N. A. (2010) · The FASEB Journal · doi:10.1096/fj.10-162230 · Cited by 113 · see citing works
Role of zinc in cellular zinc trafficking and mineralization in a murine osteoblast-like cell line
Nagata, M., & Lönnerdal, B. L. (2010) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2010.01.003 · Cited by 106 · see citing works
Thiazolidinediones Inhibit Osteoclast-Like Cell Formation and Bone Resorption in Vitro1
Okazaki, R., Toriumi, M., Fukumoto, S., Miyamoto, M., Fujita, T., TANAKA, K., & Takeuchi, Y. (1999) · Endocrinology · doi:10.1210/endo.140.11.7116 · Cited by 105 · see citing works
CXC Chemokine Ligand 2 Induced by Receptor Activator of NF-κB Ligand Enhances Osteoclastogenesis
Ha, J., Choi, H.-S., Lee, Y., Kwon, H., Song, Y. W., & Kim, H. (2010) · The Journal of Immunology · doi:10.4049/jimmunol.0902444 · Cited by 96 · see citing works
Wrzeszczyński, K. O., Varadan, V., Byrnes, J., Lum, E., Kamalakaran, S., Levine, D. A., Dimitrova, N., Zhang, M. Q., & Lucito, R. (2011) · PLoS ONE · doi:10.1371/journal.pone.0028503 · Cited by 88 · see citing works
Temporomandibular joint formation requires two distinct hedgehog-dependent steps
Purcell, P. L., Joo, B. W., Hu, J. K., Tran, P. V., Calicchio, M. L., O'Connell, D. J., Maas, R. L., & Tabin, C. J. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0908836106 · Cited by 88 · see citing works
Saxon, L., Jackson, B. F., Sugiyama, T., Lanyon, L. E., & Price, J. S. (2011) · Bone · doi:10.1016/j.bone.2011.03.683 · Cited by 84 · see citing works
Cruzan, G., Bus, J. S., Banton, M. I., Gingell, R., & Carlson, G. P. (2009) · Regulatory Toxicology and Pharmacology · doi:10.1016/j.yrtph.2009.07.002 · Cited by 81 · see citing works
Evolution of the osteoblast: skeletogenesis in gar and zebrafish
Eames, B. F., Amores, A., Yan, Y., & Postlethwait, J. H. (2012) · BMC Evolutionary Biology · doi:10.1186/1471-2148-12-27 · Cited by 78 · see citing works
Composite transcriptome assembly of RNA-seq data in a sheep model for delayed bone healing
Jäger, M., Ott, C., Grünhagen, J., Hecht, J., Schell, H., Mundlos, S., Duda, G. N., Robinson, P. N., & Lienau, J. (2011) · BMC Genomics · doi:10.1186/1471-2164-12-158 · Cited by 67 · see citing works
Moeller, C., Swindell, E. C., Kispert, A., & Gregor Eichele, C. (2003) · Development · doi:10.1242/dev.00686 · Cited by 66 · see citing works
Crockett, J. C., Schütze, N., Tosh, D., Jatzke, S., Duthie, A., Jakob, F., & Rogers, M. J. (2007) · Endocrinology · doi:10.1210/en.2007-0473 · Cited by 57 · see citing works
Niziolek, P., Warman, M. L., & Robling, A. G. (2012) · Bone · doi:10.1016/j.bone.2012.05.023 · Cited by 57 · see citing works
Self-citationDavanian, H., Stranneheim, H., Båge, T., Lagervall, M., Jansson, L. E., Lundeberg, J., & Yucel‐Lindberg, T. (2012) · PLoS ONE · doi:10.1371/journal.pone.0046440 · Cited by 55 · see citing works
Fong, L., Tan, K., Tran, C. D., Cool, J. C., Scherer, M. A., Elovaris, R. A., Coyle, P. V., Foster, B. K., Rofe, A. M., & Xian, C. J. (2009) · Bone · doi:10.1016/j.bone.2009.02.011 · Cited by 55 · see citing works
Wang, L., Shao, Y. Y., & Ballock, R. T. (2008) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.081014 · Cited by 50 · see citing works
Raymond, M. H., Schutte, B. C., Torner, J. C., Burns, T. L., & Willing, M. C. (1999) · Genomics · doi:10.1006/geno.1999.5893 · Cited by 50 · see citing works
Bone-specific overexpression of NPY modulates osteogenesis.
Matić, I. S., Matthews, B. G., Kizivat, T., Igwe, J. C., Marijanović, I., Ruohonen, S. T., Savontaus, E., Adams, D. J., & Kalajzić, I. (2012) · PubMed · Cited by 46 · see citing works
Bcr is a negative regulator of the Wnt signalling pathway
Reß, A., & Moelling, K. (2005) · EMBO Reports · doi:10.1038/sj.embor.7400536 · Cited by 44 · see citing works
Yao, B., Zhao, Y., Zhang, H., Zhang, M., Liu, M., Liu, H., & Li, J. (2012) · Biotechnology Letters · doi:10.1007/s10529-011-0841-z · Cited by 43 · see citing works
Baud’huin, M., Renault, R., Charrier, C., Riet, A. V., Brion, R., Moreau, A., Gouin, F., Duplomb, L., & Heymann, D. (2010) · Bone · doi:10.1016/j.bone.2010.04.314 · Cited by 40 · see citing works
Goto, T., Matsui, Y., Fernandes, R. J., Hanson, D. A., Kubo, T., Yukata, K., Michigami, T., Komori, T., Fujita, T., Yang, L., et al. (2006) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.020605 · Cited by 39 · see citing works
Induction of IgG3 to LPS via Toll-Like Receptor 4 Co-Stimulation
Quintana, F. J., Solomon, A., Cohen, I. R., & Nussbaum, G. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003509 · Cited by 33 · see citing works
Novel Cleft Susceptibility Genes in Chromosome 6q
Letra, A., de Menezes, R. C., Fonseca, R. F., Govil, M., McHenry, T. G., Murphy, M. J., Hennebold, J. D., Granjeiro, J. M., Castilla, E. E., Orioli, I. M., et al. (2010) · Journal of Dental Research · doi:10.1177/0022034510370004 · Cited by 32 · see citing works
Differential load-regulated global gene expression in mouse trabecular osteocytes
Wasserman, E., Webster, D. J., Kuhn, G. A., Attar-Namdar, M., Müller, R., & Bab, I. (2012) · Bone · doi:10.1016/j.bone.2012.11.017 · Cited by 24 · see citing works
Yamada, Y., Ando, F., & Shimokata, H. (2006) · International Journal of Molecular Medicine · doi:10.3892/ijmm.18.1.119 · Cited by 22 · see citing works
Li, S., Arita, M., Kopen, G. C., Phinney, D. G., & Prockop, D. J. (1998) · Matrix Biology · doi:10.1016/s0945-053x(98)90060-9 · Cited by 12 · see citing works
MDM4 overexpression contributes to synoviocyte proliferation in patients with rheumatoid arthritis
Xu, N., Wang, Y., Li, D., Chen, G., Sun, R., Zhu, R., Sun, S., Liu, H., Yang, G., & Dong, T. (2010) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2010.09.069 · Cited by 11 · see citing works
Evaluation of bone‐derived and marrow‐derived vascular endothelial cells by microarray analysis
Sosnoski, D. M., & Gay, C. V. (2007) · Journal of Cellular Biochemistry · doi:10.1002/jcb.21307 · Cited by 7 · see citing works
Flagged first, then by citations
2 of the 79 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
An osteocalcin-deficient mouse strain without endocrine abnormalities
Diegel, C. R., Hann, S., Ayturk, U. M., Hu, J. C. W., Lim, K., Droscha, C. J., Madaj, Z., Foxa, G. E., Izaguirre, I., Core, V. V. A. T., et al. (2020) · PLoS Genetics · doi:10.1371/journal.pgen.1008361 · Cited by 136 · see citing works
Self-citationYoulten, S. E., Kemp, J. P., Logan, J. G., Ghirardello, E. J., Sergio, C. M., Dack, M. R. G., Guilfoyle, S. E., Leitch, V. D., Butterfield, N. C., Komla-Ebri, D., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-22517-1 · Cited by 133 · see citing works
Yue, R., Shen, B., & Morrison, S. J. (2016) · eLife · doi:10.7554/elife.18782 · Cited by 132 · see citing works
Islam, M. N., Masud, M. K., Haque, M. H., Hossain, M. S. A., Yamauchi, Y., Nguyen, N., & Shiddiky, M. J. A. (2017) · Small Methods · doi:10.1002/smtd.201700131 · Cited by 115 · see citing works
A Normative Study of the Synovial Fluid Proteome from Healthy Porcine Knee Joints
Bennike, T. B., Ayturk, U. M., Haslauer, C. M., Froehlich, J. W., Proffen, B. L., Barnaby, O. S., Birkelund, S., Murray, M. M., Warman, M. L., Stensballe, A., et al. (2014) · Journal of Proteome Research · doi:10.1021/pr500587x · Cited by 95 · see citing works
Self-citationTargeting the LRP5 Pathway Improves Bone Properties in a Mouse Model of Osteogenesis Imperfecta
Jacobsen, C. M., Barber, L. A., Ayturk, U. M., Roberts, H. J., Deal, L. E., Schwartz, M. A., Weis, M., Eyre, D. R., Zurakowski, D., Robling, A. G., et al. (2014) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.2198 · Cited by 83 · see citing works
Self-citationEffects of Age and Estrogen on Skeletal Gene Expression in Humans as Assessed by RNA Sequencing
Farr, J. N., Roforth, M. M., Fujita, K., Nicks, K. M., Cunningham, J. M., Atkinson, E. J., Therneau, T. M., McCready, L. K., Peterson, J. M., Drake, M. T., et al. (2015) · PLOS ONE · doi:10.1371/journal.pone.0138347 · Cited by 75 · see citing works
Sclerostin Inhibition Reverses Skeletal Fragility in an Lrp5-Deficient Mouse Model of OPPG Syndrome
Kedlaya, R., Veera, S. S., Horan, D. J., Moss, R. E., Ayturk, U. M., Jacobsen, C. M., Bowen, M. E., Pászty, C., Warman, M. L., & Robling, A. G. (2013) · Science Translational Medicine · doi:10.1126/scitranslmed.3006627 · Cited by 77 · see citing works
Self-citationChang, M.-K., Krämer, I., Keller, H., Gooi, J. H., Collett, C., Jenkins, D., Ettenberg, S. A., Cong, F., Halleux, C. M., & Kneissel, M. (2013) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.2059 · Cited by 76 · see citing works
Kelly, N. H., Schimenti, J. C., Ross, F. P., & van der Meulen, M. C. H. (2016) · Bone · doi:10.1016/j.bone.2016.02.007 · Cited by 72 · see citing works
A method for isolating high quality RNA from mouse cortical and cancellous bone
Kelly, N. H., Schimenti, J. C., Ross, F. P., & van der Meulen, M. C. H. (2014) · Bone · doi:10.1016/j.bone.2014.07.022 · Cited by 69 · see citing works
Regulation of Osteoblast Metabolism by Wnt Signaling
Moorer, M. C., & Riddle, R. C. (2018) · Endocrinology and Metabolism · doi:10.3803/enm.2018.33.3.318 · Cited by 58 · see citing works
Single-Cell RNA Sequencing of Calvarial and Long-Bone Endocortical Cells
Ayturk, U. M., Scollan, J. P., Aytürk, D. G., Suh, E. S., Vesprey, A., Jacobsen, C. M., Pajevic, P. D., & Warman, M. L. (2020) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.4052 · Cited by 57 · see citing works
Self-citationTan, S. H., Senarath-Yapa, K., Chung, M. T., Longaker, M. T., Wu, J. Y., & Nusse, R. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1420463111 · Cited by 57 · see citing works
Bowen, M. E., Ayturk, U. M., Kurek, K. C., Yang, W., & Warman, M. L. (2014) · PLoS Genetics · doi:10.1371/journal.pgen.1004364 · Cited by 57 · see citing works
Self-citationAyturk, U. M. (2019) · Current Osteoporosis Reports · doi:10.1007/s11914-019-00517-x · Cited by 52 · see citing works
Self-citationSieker, J. T., Ayturk, U. M., Proffen, B. L., Weissenberger, M. H., Kiapour, A. M., & Murray, M. M. (2016) · Arthritis & Rheumatology · doi:10.1002/art.39631 · Cited by 49 · see citing works
Self-citationHebb, J. H., Ashley, J. W., McDaniel, L. D., Lopas, L. A., Tobias, J. W., Hankenson, K. D., & Ahn, J. (2017) · Journal of Orthopaedic Research® · doi:10.1002/jor.23652 · Cited by 45 · see citing works
The Future of Biomarkers in Veterinary Medicine: Emerging Approaches and Associated Challenges
Perera, T. R. W., Skerrett-Byrne, D. A., Gibb, Z., Nixon, B., & Swegen, A. (2022) · Animals · doi:10.3390/ani12172194 · Cited by 44 · see citing works
Sieker, J. T., Proffen, B. L., Waller, K. A., Chin, K. E., Karamchedu, N. P., Akelman, M. R., Perrone, G. S., Kiapour, A. M., Konrad, J., Murray, M. M., et al. (2017) · Journal of Orthopaedic Research® · doi:10.1002/jor.23644 · Cited by 43 · see citing works
β-Catenin Directs Long-Chain Fatty Acid Catabolism in the Osteoblasts of Male Mice
Frey, J. L., Kim, S. P., Zhu, L., Wolfgang, M. J., & Riddle, R. C. (2017) · Endocrinology · doi:10.1210/en.2017-00850 · Cited by 42 · see citing works
CYR61/CCN1 Regulates Sclerostin Levels and Bone Maintenance
Zhao, G., Huang, B., Rigueur, D., Wang, W., Bhoot, C., Charles, K. R., Baek, J., Mohan, S., Jiang, J., & Lyons, K. M. (2018) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.3394 · Cited by 40 · see citing works
Chermside‐Scabbo, C. J., Harris, T. L., Brodt, M. D., Brænne, I., Zhang, B., Farber, C. R., & Silva, M. J. (2020) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.4031 · Cited by 41 · see citing works
Lubricin Restoration in a Mouse Model of Congenital Deficiency
Hill, A., Waller, K. A., Cui, Y., Allen, J. M., Smits, P. J., Zhang, L. X., Ayturk, U. M., Hann, S., Lessard, S. G., Zurakowski, D., et al. (2015) · Arthritis & Rheumatology · doi:10.1002/art.39276 · Cited by 39 · see citing works
Self-citationGenetic Approaches To Identifying Novel Osteoporosis Drug Targets
Brommage, R. (2015) · Journal of Cellular Biochemistry · doi:10.1002/jcb.25179 · Cited by 35 · see citing works
The Role of Omega-3 Polyunsaturated Fatty Acids from Different Sources in Bone Development
Rozner, R., Vernikov, J., Griess-Fishheimer, S., Travinsky, T., Penn, S., Schwartz, B., Mesilati‐Stahy, R., Argov-Argaman, N., Shahar, R., & Ornan, E. M. (2020) · Nutrients · doi:10.3390/nu12113494 · Cited by 35 · see citing works
Morse, A., Schindeler, A., McDonald, M. M., Kneissel, M., Krämer, I., & Little, D. G. (2017) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.3330 · Cited by 32 · see citing works
Bai, M., Han, Y., Wu, Y., Liao, J., Li, L., Wang, L., Li, Q., Xing, W., Chen, L., Zou, W., et al. (2019) · PLoS Biology · doi:10.1371/journal.pbio.3000350 · Cited by 32 · see citing works
The Interaction Between Intracellular Energy Metabolism and Signaling Pathways During Osteogenesis
Ye, J., Xiao, J., Wang, J., Ma, Y., Zhang, Y., Zhang, Q., Zhang, Z., & Yin, H. (2022) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2021.807487 · Cited by 30 · see citing works
Marinkovic, M., Dai, Q., Gonzalez, A. O., Tran, O. N., Block, T. J., Harris, S. E., Salmon, A. B., Yeh, C., Dean, D. D., & Chen, X. (2022) · Matrix Biology · doi:10.1016/j.matbio.2022.06.004 · Cited by 30 · see citing works
The role of Meteorin‐like in skeletal development and bone fracture healing
Rong, H., Balu, A. R., Molitoris, K. H., White, J. P., Robling, A. G., Ayturk, U. M., & Baht, G. S. (2022) · Journal of Orthopaedic Research® · doi:10.1002/jor.25286 · Cited by 29 · see citing works
Self-citationInsights Into the Mechanisms of Sclerostin Action in Regulating Bone Mass Accrual
Williams, B. O. (2013) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.2154 · Cited by 29 · see citing works
Isolation and characterization of human osteoblasts from needle biopsies without in vitro culture
Fujita, K., Roforth, M. M., Atkinson, E. J., Peterson, J. M., Drake, M. T., McCready, L. K., Farr, J. N., Monroe, D. G., & Khosla, S. (2013) · Osteoporosis International · doi:10.1007/s00198-013-2529-9 · Cited by 29 · see citing works
Transcriptional profiling of synovium in a porcine model of early post‐traumatic osteoarthritis
Sieker, J. T., Proffen, B. L., Waller, K. A., Chin, K. E., Karamchedu, N. P., Akelman, M. R., Perrone, G. S., Kiapour, A. M., Konrad, J., Fleming, B. C., et al. (2018) · Journal of Orthopaedic Research® · doi:10.1002/jor.23876 · Cited by 28 · see citing works
Curtis, K. J., Coughlin, T. R., Mason, D. E., Boerckel, J. D., & Niebur, G. L. (2017) · Bone · doi:10.1016/j.bone.2017.11.007 · Cited by 26 · see citing works
Reply to Lrp5 regulation of bone mass and gut serotonin synthesis
Cui, Y., Niziolek, P., MacDonald, B. T., Alenina, N. V., Matthes, S., Jacobsen, C. M., Conlon, R. A., Brommage, R., Powell, D. R., He, X., et al. (2014) · Nature Medicine · doi:10.1038/nm.3697 · Cited by 28 · see citing works
Self-citationPei, S., Parthasarathy, S., Parajuli, A., Martinez, J. R., Lv, M., Jiang, S., Wu, D., Wei, S., Lu, X. L., Farach‐Carson, M. C., et al. (2019) · Bone · doi:10.1016/j.bone.2019.115078 · Cited by 27 · see citing works
Lineage-specific differences and regulatory networks governing human chondrocyte development
Richard, D. J., Pregizer, S. K., Venkatasubramanian, D., Raftery, R. M., Muthuirulan, P., Liu, Z., Capellini, T. D., & Craft, A. M. (2023) · eLife · doi:10.7554/elife.79925 · Cited by 25 · see citing works
Frizzled‐4 is required for normal bone acquisition despite compensation by Frizzled‐8
Kushwaha, P., Kim, S., Foxa, G. E., Michalski, M. N., Williams, B. O., Tomlinson, R. E., & Riddle, R. C. (2020) · Journal of Cellular Physiology · doi:10.1002/jcp.29563 · Cited by 25 · see citing works
Robinson, J. W., Blixt, N. C., Norton, A., Mansky, K. C., Ye, Z., Aparicio, C., Wagner, B. M., Benton, A. M., Warren, G. L., Khosla, S., et al. (2020) · Bone · doi:10.1016/j.bone.2020.115320 · Cited by 22 · see citing works
Functional interaction between Wnt and Bmp signaling in periosteal bone growth
Song, D., He, G., Shi, Y., Ni, J., & Long, F. (2021) · Scientific Reports · doi:10.1038/s41598-021-90324-1 · Cited by 20 · see citing works
Barad, M., Csukasi, F., Bosakova, M. K., Martin, J. H., Zhang, W., Taylor, S. P., Lachman, R. S., Zieba, J. T., Bamshad, M. J., Nickerson, D. A., et al. (2020) · EBioMedicine · doi:10.1016/j.ebiom.2020.103075 · Cited by 18 · see citing works
Ayturk, U. M., Sieker, J. T., Haslauer, C. M., Proffen, B. L., Weissenberger, M. H., Warman, M. L., Fleming, B. C., & Murray, M. M. (2020) · PLoS ONE · doi:10.1371/journal.pone.0229449 · Cited by 17 · see citing works
Self-citationHong, A. R., Kim, K., Lee, J. Y., Yang, J., Kim, J. H., Shin, C. S., & Kim, S. W. (2020) · Endocrinology and Metabolism · doi:10.3803/enm.2020.35.2.456 · Cited by 17 · see citing works
Turajane, K., Ji, G., Chinenov, Y. V., Chao, M., Ayturk, U. M., Suhardi, V. J., Greenblatt, M. B., Ivashkiv, L. B., Bostrom, M. P., & Yang, X. (2021) · JBMR Plus · doi:10.1002/jbm4.10535 · Cited by 15 · see citing works
Self-citationThe Osteocyte Transcriptome: Discovering Messages Buried Within Bone
Wee, N. K. Y., Sims, N. A., & Morello, R. (2021) · Current Osteoporosis Reports · doi:10.1007/s11914-021-00708-5 · Cited by 15 · see citing works
Bone mechanobiology in mice: toward single-cell in vivo mechanomics
Scheuren, A. C., Wehrle, E., Flohr, F. R., & Müller, R. (2017) · Biomechanics and Modeling in Mechanobiology · doi:10.1007/s10237-017-0935-1 · Cited by 15 · see citing works
Bullock, W. A., Hoggatt, A. M., Horan, D. J., Lewis, K. J., Yokota, H., Hann, S., Warman, M. L., Sebastian, A., Loots, G. G., Pavalko, F. M., et al. (2019) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.3812 · Cited by 13 · see citing works
Self-citationNew and Old Osteocytic Cell Lines and 3D Models
Pajevic, P. D. (2020) · Current Osteoporosis Reports · doi:10.1007/s11914-020-00613-3 · Cited by 11 · see citing works
Chermside‐Scabbo, C. J., Shuster, J. T., Erdmann-Gilmore, P., Tycksen, E., Zhang, Q., Townsend, R. R., & Silva, M. J. (2024) · Aging · doi:10.18632/aging.206131 · Cited by 10 · see citing works
Lv, Z., Zhang, J., Liang, S., Zhou, C., Hu, D. Z., Brooks, D. J., Bouxsein, M., Lanske, B., Kostenuik, P. J., Gori, F., et al. (2023) · JCI Insight · doi:10.1172/jci.insight.161932 · Cited by 11 · see citing works
Self‐Propelled Nanoswimmers in Biomedical Sensing
Chen, Q., & Liang, K. (2023) · Advanced Sensor Research · doi:10.1002/adsr.202300056 · Cited by 10 · see citing works
Lin, J., Zheng, Z., Liu, J., Yang, G., Leng, L., Wang, H., Qiu, G., & Wu, Z. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.766815 · Cited by 8 · see citing works
Transcriptome profile of highly osteoblastic/cementoblastic periodontal ligament cell clones
Saito, M. T., Mofatto, L. S., Albiero, M. L., Casati, M. Z., Sallum, E. A., Nociti, F. H., & Silvério, K. G. (2020) · Journal of Applied Oral Science · doi:10.1590/1678-7757-2020-0242 · Cited by 8 · see citing works
Sontam, D. M., Firth, E. C., Tsai, P., Vickers, M. H., & O'Sullivan, J. M. (2015) · Physiological Reports · doi:10.14814/phy2.12568 · Cited by 8 · see citing works
A Memory of Early Life Physical Activity Is Retained in Bone Marrow of Male Rats Fed a High-Fat Diet
Sontam, D. M., Vickers, M. H., Firth, E. C., & O'Sullivan, J. M. (2017) · Frontiers in Physiology · doi:10.3389/fphys.2017.00476 · Cited by 8 · see citing works
He, A., Tian, S., Kopper, O., Horan, D. J., Chen, P., Bronson, R. T., Sheng, R., Wu, H., Sui, L., Zhou, K., et al. (2023) · PLoS Biology · doi:10.1371/journal.pbio.3002353 · Cited by 7 · see citing works
Self-citationTranscriptomics: a Solution for Renal Osteodystrophy?
Martin, A., & David, V. (2020) · Current Osteoporosis Reports · doi:10.1007/s11914-020-00583-6 · Cited by 7 · see citing works
Orriss, I. R., Lanham, S., Savery, D., Greene, N. D. E., Stanier, P., Oreffo, R. O. C., Copp, A. J., & Galea, G. L. (2018) · Scientific Reports · doi:10.1038/s41598-018-21718-x · Cited by 7 · see citing works
The role of Clec11a in bone construction and remodeling
Xu, K., Huang, R., Wen, R., Yang, Y., Cheng, Y., & Chang, B. (2024) · Frontiers in Endocrinology · doi:10.3389/fendo.2024.1429567 · Cited by 6 · see citing works
Recent regulatory changes shaped human facial and vocal anatomyPreprint
Gokhman, D., Agranat-Tamir, L., Housman, G., Nissim‐Rafinia, M., Nieves-Colón, M. A., Gu, H., Ferrando-Bernal, M., Gelabert, P., Lipende, I. F., Quillen, E. E., et al. (2017) · MPG.PuRe (Max Planck Society) · Cited by 8 · see citing works
Wang, Y., Di, Y., Li, Y., Lu, J., Ji, B., Zhang, Y., Chen, Z., Chen, S., Liu, B., & Tang, R. (2024) · Chinese Medical Journal · doi:10.1097/cm9.0000000000003261 · Cited by 5 · see citing works
Controlled mechanical loading affects the osteocyte transcriptome in porcine trabecular bone in situ
Machireddy, M., Oberman, A. G., Debiase, L., Stephens, M., Li, J., Littlepage, L. E., & Niebur, G. L. (2024) · Bone · doi:10.1016/j.bone.2024.117028 · Cited by 4 · see citing works
Genetic and Gene Expression Resources for Osteoporosis and Bone Biology Research
Kaya, S., Alliston, T. N., & Evans, D. S. (2023) · Current Osteoporosis Reports · doi:10.1007/s11914-023-00821-7 · Cited by 5 · see citing works
Using mouse genetics to understand human skeletal disease
Youlten, S. E., & Baldock, P. A. J. (2019) · Bone · doi:10.1016/j.bone.2019.02.015 · Cited by 5 · see citing works
Spatz, J., Ko, F. C., Ayturk, U. M., Warman, M. L., & Bouxsein, M. (2021) · PLoS ONE · doi:10.1371/journal.pone.0250715 · Cited by 4 · see citing works
Self-citationThe temporal expression of adipokines during spinal fusion
Virk, S. S., Bertone, A. L., Hussein, H. H., Toth, J. M., Kaido, M., & Khan, S. N. (2017) · The Spine Journal · doi:10.1016/j.spinee.2017.06.019 · Cited by 4 · see citing works
Tauer, J. T., Thiele, T., Julien, C., Ofer, L., Zaslansky, P., Shahar, R., & Willie, B. M. (2024) · Bone · doi:10.1016/j.bone.2024.117125 · Cited by 3 · see citing works
Nutrient uptake and metabolism in osteoblasts
Karner, C. M., & Long, F. (2023) · Current Opinion in Endocrine and Metabolic Research · doi:10.1016/j.coemr.2023.100447 · Cited by 3 · see citing works
Identification of mRNAs Related to Tibial Cartilage Development of Yorkshire Piglets
Feng, S., Du, X., Wang, C., Ye, D., Ma, G., Zhao, S., Wang, H., & Liu, X. (2019) · BioMed Research International · doi:10.1155/2019/2365416 · Cited by 2 · see citing works
Turajane, K., Ji, G., Chinenov, Y. V., Chao, M., Ayturk, U. M., Greenblatt, M. B., Ivashkiv, L. B., Bostrom, M. P., & Yang, X. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.12.27.424462 · Cited by 2 · see citing works
Self-citationMouse Model of OPPG Syndrome Sclerostin Inhibition Reverses Skeletal Fragility in an Lrp5-Deficient
Kedlaya, R. (2013) · Cited by 1 · see citing works
The Variety of Osteocyte Function
O’Brien, C. A. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-800571-2.00006-2 · Cited by 1 · see citing works
Sebastian, A., & Loots, G. G. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-804182-6.00005-8 · Cited by 1 · see citing works
Inflammatory Diseases – An Analysis of the Underlying Biological Triggers
Bennike, T. B. (2015) · VBN Forskningsportal (Aalborg Universitet) · doi:10.5278/vbn.phd.engsci.00012
Second Herbert Fleisch Workshop, 2016
Maes, C., Helfrich, M., Bouillon, R., & Martin, T. J. (2016) · IBMS BoneKEy · doi:10.1038/bonekey.2016.51
Hudson, B. D., & Loots, G. G. (2012) · Elsevier eBooks · doi:10.1016/b978-0-12-387829-8.00008-1
Transcriptional Profiling for Genetic Assessment
Sebastian, A., & Loots, G. G. (2018) · Primer on the metabolic bone diseases and disorders of mineral metabolism · doi:10.1002/9781119266594.ch45
Transcriptome Analysis of Wnt Signaling Pathway in Pediatric Hepatocellular Carcinoma
Rabbani, N. (2019) · Digital Access to Scholarship at Harvard (DASH) (Harvard University)
The Role of Wnt Signaling in Bone Mechanotransduction
Bullock, W. A. (2019) · IUScholarWorks (Indiana University) · doi:10.7912/c2/2122
Nucleic acid based molecular markers in the diagnosis of cancer
Kumari, A., & Kumar, V. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-22300-6.00048-6
Ayturk, U. M., Rogers, M., Seide, K., Oliver, D., Chinenov, Y. V., Aydın, E., Carter, E. M., & Raggio, C. (2026) · JBMR Plus · doi:10.1093/jbmrpl/ziag088
Self-citationDaponte, V., Gavva, C., Kapfhamer, D. J., Roberts, J. L., Henke, K., & Drissi, H. (2026) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2026.1868995
Ziouti, F., Azeem, M., Rummler, M., Rosołowski, M., Zapata, G. A., Rindt, W., Julien, C., Tauer, J. T., Cheng, W.-H., Beck, S., et al. (2026) · Bone · doi:10.1016/j.bone.2026.118083
- Authors
- Ayturk, U. M., Jacobsen, C. M., Christodoulou, D. C., Gorham, J., Seidman, J. G., Seidman, C. E., Robling, A. G., & Warman, M. L.
- Affiliations
- Boston Children's Hospital (US)
- Harvard University (US)
- Howard Hughes Medical Institute (US)
- Indiana University School of Medicine (US)
- Corresponding author
- Warman, Matthew L
- ORCID
- Venue
- Journal of Bone and Mineral Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2013-04-02
- Volume
- 28
- Issue
- 10
- Pages
- 2081-2093
- ISSN
- 0884-0431 (ISSN-L)
- 1523-4681
- PMC
- PMC3743099
- OpenAlex
- W2171092805
- Cited by
- 84
- References
- 79
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 5 · 2023: 6 · 2022: 4 · 2021: 6 · 2020: 13 · 2019: 8 · 2018: 5 · 2017: 11 · 2016: 4 · 2015: 6 · 2014: 6 · 2013: 5 · 2012: 1
- FWCI
- 7.16
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Journal of Bone and Mineral Research (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- IUScholarWorks (Indiana University) (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- Broad Institute
- National Institutes of Health (1u01hl098166-01, UL1TR000439)
- National Heart, Lung, and Blood Institute (U01-HL098166)
- National Institute of General Medical Sciences (P20GM104937)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R21AR062326, K08AR063813, R01AR053237)
- National Center for Advancing Translational Sciences
- Topics
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Keywords
- LRP5, mRNA expression, high bone mass, collagenase digestion, tibia, differentially expressed genes, bone mass regulation, mouse model
- MeSH terms
- Animals, Bone Marrow, Computer Simulation, Gene Expression Regulation, Humans, Male, Mutation, RNA, Messenger, Tibia, Reproducibility of Results, Gene Library, Sequence Analysis, RNA, Muscle, Skeletal, Diaphyses, Mice, Low Density Lipoprotein Receptor-Related Protein-5
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
