Matrix mineralization and the differentiation of osteocyte-like cells in culture
Abstract
Osteocyte-like cells were prepared by sequentially treating calvaria from newborn rats with collagenase and chelating agents. On a reconstituted gel of basement membrane components, cells from the third collagenase digest displayed a round shape and expressed the highest level of alkaline phosphatase with minimal osteocalcin deposition into the matrix. On the other hand, cells derived from the interior after EDTA treatment exhibited well-developed dendritic cell processes and expressed essentially no alkaline phosphatase. The latter population also showed quite distinct characteristics such as higher extracellular activities of casein kinase II and ecto-5′-nucleotidase and the extracellular accumulation of a large amount of osteocalcin associated with mineral. These diverse phenotypic and protein expressions as well as the sites from which each population of cells were recovered strongly suggest that we have isolated osteoblastic and osteocytic cells. Bone sialoprotein II was extracellularly phosphorylated by casein kinase II in osteocytic cells but not in osteoblastic cells. We discuss the possibility that differentiation of young osteocytes from osteoblasts may facilitate the biochemical sequence of mineral deposition in the bone matrix.
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Osteocyte markers including dentine matrix protein 1 (DMP1) and sclerostin are highly expressed in mature osteocytes.
“On the other hand, osteocyte markers including dentine matrix protein 1 (DMP1) and sclerostin are highly expressed [8, 62–64].”
From Biology of Bone Tissue: Structure, Function, and Factors That Influence Bone Cells (2015) · cites this paper as [62]
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- Authors
- Mikuni-Takagaki, Y., Kakai, Y., Satoyoshi, M., Kawano, E., Suzuki, Y., Kawase, T., & Saito, S.
- Affiliations
- Department of Oral Biochemistry, Kanagawa Dental College, Yokosuka, Japan
- Kanagawa Dental University (JP)
- ORCID
- Saito, Shigeru
- Venue
- Journal of Bone and Mineral Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1995-02-01
- Volume
- 10
- Issue
- 2
- Pages
- 231-242
- ISSN
- 0884-0431 (ISSN-L)
- 1523-4681
- OpenAlex
- W2029859781
- Cited by
- 144
- References
- 46
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 5 · 2020: 9 · 2019: 6 · 2018: 6 · 2017: 2 · 2016: 7 · 2015: 8 · 2014: 10 · 2013: 6 · 2012: 8
- FWCI
- 2.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Journal of Bone and Mineral Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Hayashi foundation to Mikuni-Takagaki
- Ministry of Science, Education and Culture of Japan
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Alkaline Phosphatase Research Studies
- Keywords
- osteocyte differentiation, osteogenic differentiation, osteocyte-like cells, osteocalcin, bone matrix mineralization, casein kinase 2, ecto-5'-nucleotidase, alkaline phosphatase, collagenase digestion, EDTA treatment, calvaria, bone matrix deposition
- MeSH terms
- Alkaline Phosphatase, Animals, Animals, Newborn, Basement Membrane, Bone Matrix, Calcification, Physiologic, Cell Differentiation, Cell Separation, Cells, Cultured, Dendrites, DNA-Binding Proteins, Edetic Acid, Frontal Bone, Osteoblasts, Osteocytes, Parietal Bone, Phenotype, Phosphorylation, Osteocalcin, 5'-Nucleotidase, Rats, Sprague-Dawley, Protein Serine-Threonine Kinases, Casein Kinase II, Rats
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