The DOI matches its Crossref record.
Conditions controlling the proliferation of haemopoietic stem cells in vitro
Abstract
A liquid culture system is described whereby proliferation of haemopoietic stem cells (CFU‐S), production of granulocyte precursor cells (CFU‐C), and extensive granulopoiesis can be maintained in vitro for several months. Such cultures consist of adherent and non‐adherent populations of cells. The adherent population contains phagocytic mononuclear cells, “epithelial” cells, and “giant fat” cells. The latter appear to be particularly important for stem cell maintenance and furthermore there is a strong tendency for maturing granulocytes to selectively cluster in and around areas of “giant fat” cell aggregations. By “feeding” the cultures at weekly intervals, between 10 to 15 “population doublings” of functionally normal CFU‐S regularly occurs. Increased “population doublings” may be obtained by feeding twice weekly. The cultures show initially extensive granulopoiesis followed, in a majority of cases, by an accumulation of blast cells. Eventually both blast cells and granulocytes decline and the cultures contain predominantly phagocytic mononuclear cells. Culturing at 33°C leads to the development of a more profuse growth of adherent cells and these cultures show better maintenance of stem cells and increased cell density. When tested for colony stimulating activity (CSA) the cultures were uniformly negative. Addition of exogenous CSA caused a rapid decline in stem cells, reduced granulopoiesis and an accumulation of phagocytic mononuclear cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
A metalloproteinase disintegrin that releases tumour-necrosis factor-α from cells
Black, R. A., Rauch, C. T., Kozlosky, C. J., Peschon, J. J., Slack, J. L., Wolfson, M. F., Castner, B. J., Stocking, K. L., Reddy, P., Srinivasan, S., et al. (1997) · Nature · doi:10.1038/385729a0 · Cited by 3,149
Mesenchymal stem cells as trophic mediators
Caplan, A. I., & Dennis, J. E. (2006) · Journal of Cellular Biochemistry · doi:10.1002/jcb.20886 · Cited by 2,992
The bone marrow niche for haematopoietic stem cells
Morrison, S. J., & Scadden, D. T. (2014) · Nature · doi:10.1038/nature12984 · Cited by 2,451
Mechanisms involved in the therapeutic properties of mesenchymal stem cells
da Silva Meirelles, L., Fontes, A. M., Covas, D. T., & Caplan, A. I. (2009) · Cytokine & Growth Factor Reviews · doi:10.1016/j.cytogfr.2009.10.002 · Cited by 1,436
Mesenchymal stromal cells: new directions
Keating, A. (2012) · Cell Stem Cell · doi:10.1016/j.stem.2012.05.015 · Cited by 778
Hereditary Anemias of the Mouse: A Review for Geneticists
Russell, E. S. (1979) · Advances in Genetics · doi:10.1016/s0065-2660(08)60549-0 · Cited by 766
Hematopoietic Cell Regulation by Rac1 and Rac2 Guanosine Triphosphatases
Gu, Y., Filippi, M.-D., Cancelas, J. A., Siefring, J. E., Williams, E. P., Jasti, A. C., Harris, C. E., Lee, A. W., Prabhakar, R., Atkinson, S. J., et al. (2003) · Science · doi:10.1126/science.1088485 · Cited by 510
Frenette, P. S., Pinho, S., Lucas, D., & Scheiermann, C. (2013) · Annual Review of Immunology · doi:10.1146/annurev-immunol-032712-095919 · Cited by 462
Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse
Williams, D. A., Lemischka, I. R., Nathan, D. G., & Mulligan, R. C. (1984) · Nature · doi:10.1038/310476a0 · Cited by 388
Anklesaria, P., Teixidó, J., Laiho, M., Pierce, J. H., Greenberger, J. S., & Massagué, J. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.9.3289 · Cited by 252
Impaired Ability of Bone Marrow Stromal Cells to Support B-Lymphopoiesis With Age
Stephan, R. P., Reilly, C. R., & Witte, P. L. (1998) · Blood · doi:10.1182/blood.v91.1.75 · Cited by 197
Osteoclasts are important for bone angiogenesis
Cackowski, F. C., Anderson, J. L., Patrene, K. D., Choksi, R. J., Shapiro, S. D., Windle, J. J., Blair, H. C., & Roodman, G. D. (2010) · Blood · doi:10.1182/blood-2009-08-237628 · Cited by 188
Schrader, J. W., Lewis, S. J., Clark-Lewis, I., & Culvenor, J. G. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.1.323 · Cited by 139
Evidences of Early Senescence in Multiple Myeloma Bone Marrow Mesenchymal Stromal Cells
André, T., Meuleman, N., Stamatopoulos, B., De Bruyn, C., Pieters, K., Bron, D., & Lagneaux, L. (2013) · PLoS ONE · doi:10.1371/journal.pone.0059756 · Cited by 107
Gu, Y., Jasti, A. C., Jansen, M., & Siefring, J. E. (2005) · Blood · doi:10.1182/blood-2004-04-1604 · Cited by 106
Dexter, T. M., Spooncer, E., Toksoz, D., & Lajtha, L. G. (1980) · Journal of Supramolecular Structure · doi:10.1002/jss.400130410 · Cited by 81
Self-citationProliferation of Bone Marrow Pro-B Cells Is Dependent on Stimulation by the Pituitary/Thyroid Axis
Foster, M. P., Montecino-Rodriguez, E., & Dorshkind, K. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.163.11.5883 · Cited by 75
Wang, Y., Liu, L., & Zhou, D. (2011) · Radiation Research · doi:10.1667/rr2727.1 · Cited by 67
Kang, J. S., Yoon, Y. D., Han, M. H., Han, S.-B., Lee, K., Kang, M. R., Moon, E.-Y., Jeon, Y. J., Park, S.-K., & Kim, H. M. (2005) · Biochemical Pharmacology · doi:10.1016/j.bcp.2005.10.009 · Cited by 43
The bone marrow hematopoietic niche and its adaptation to infection
Gomes, A. C., Saraiva, M., & Gomes, M. S. (2021) · Seminars in Cell & Developmental Biology · doi:10.1016/j.semcdb.2020.05.014 · Cited by 18
Metadata
- Authors
- Dexter, T. M., Allen, T. D., & Lajtha, L. G.
- Venue
- Journal of Cellular Physiology
- Publisher
- Wiley
- Published
- 1977-06
- Volume
- 91
- Issue
- 3
- Pages
- 335-344
- ISSN
- 0021-9541 (ISSN-L)
- 1097-4652
- OpenAlex
- W2086793358
- Cited by
- 2,066
- References
- 0
- FWCI
- 24.36
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
