The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Microscale autoradiographic method for the qualitative and quantitative analysis of apoptotic DNA fragmentation
Abstract
A method combining the advantages of electrophoretic DNA fractionation and autoradiography is described for the qualitative and quantitative analysis of internucleosomal DNA fragmentation that occurs during apoptosis, or “programmed cell death”. This procedure utilizes terminal transferase enzyme to uniformly add one molecule of [α 32 P]‐to the 3′‐of DNA fragments. Following gel electrophoresis and autoradiographic analysis, the total amount of radiolabel incorporated into the low molecular weight DNA fraction can be quantitated and used to estimate the degree of apoptotic DNA fragmentation in any given sample. This method requires as little as 15 ng of total cellular DNA and increases the sensitivity of apoptotic DNA detection by at least 100‐fold over the widely used ethidium bromide staining method. The procedure should prove valuable for the analysis of apoptosis in minute quantities of tissues and cultured cells. © 1993 Wiley‐Liss, Inc.
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Cites
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
Wyllie, A. H. (1980) · Nature · doi:10.1038/284555a0 · Cited by 4,504
Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death
Hockenbery, D., Nuñez, G., Milliman, C., Schreiber, R. D., & Korsmeyer, S. J. (1990) · Nature · doi:10.1038/348334a0 · Cited by 3,720
Social controls on cell survival and cell death
Raff, M. C. (1992) · Nature · doi:10.1038/356397a0 · Cited by 2,604
Isolation of High‐Molecular‐Weight DNA from Mammalian Cells
Gross‐Bellard, M., Oudet, P., & Chambon, P. (1973) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1973.tb02881.x · Cited by 1,666
Chen, Y., & Wu, H. (2010) · Chinese Science Bulletin · doi:10.1007/s11434-010-3275-4 · Cited by 1,594
Erythropoietin Retards DNA Breakdown and Prevents Programmed Death in Erythroid Progenitor Cells
Koury, M. J., & Bondurant, M. C. (1990) · Science · doi:10.1126/science.2326648 · Cited by 812
Activation of Programmed Cell Death in the Rat Ventral Prostate after Castration*
KYPRIANOU, N., & ISAACS, J. T. (1988) · Endocrinology · doi:10.1210/endo-122-2-552 · Cited by 720
INVOLVEMENT OF APOPTOSIS IN OVARIAN FOLLICULAR ATRESIA AND POSTOVULATORY REGRESSION
Tilly, J. L., Kowalski, K. I., Johnson, A. L., & Hsueh, A. J. W. (1991) · Endocrinology · doi:10.1210/endo-129-5-2799 · Cited by 657
Self-citationEndogenous endonuclease-induced DNA fragmentation: an early event in cell-mediated cytolysis.
Duke, R. C., Chervenak, R., & Cohen, J. J. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.20.6361 · Cited by 606
Cell death: a new classification separating apoptosis from necrosis
Wyllie, A. H. (1981) · Cell death in biology and pathology · doi:10.1007/978-94-011-6921-9_2 · Cited by 563
Cited by
Activation and Cleavage of Caspase-3 in Apoptosis Induced by Experimental Cerebral Ischemia
Namura, S., Zhu, J., Fink, K., Endres, M., Srinivasan, A., Tomaselli, K. J., Yuan, J., & Moskowitz, M. A. (1998) · The Journal of Neuroscience · doi:10.1523/jneurosci.18-10-03659.1998 · Cited by 893
Apoptosis in human acute myocardial infarction.
Saraste, A., Pulkki, K., Kallajoki, M., Henriksen, K., Parvinen, M., & Voipio-Pulkki, L.-M. (1997) · Circulation · doi:10.1161/01.cir.95.2.320 · Cited by 825
Ovarian Follicle Atresia: A Hormonally Controlled Apoptotic Process*
HSUEH, A. J. W., BILLIG, H., & TSAFRIRI, A. (1994) · Endocrine Reviews · doi:10.1210/edrv-15-6-707 · Cited by 787
Self-citationAngiotensin II type 2 receptor mediates programmed cell death.
Yamada, T., Horiuchi, M., & Dzau, V. J. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.1.156 · Cited by 697
Ischemic Brain Injury is Mediated by the Activation of Poly(ADP-Ribose)Polymerase
Endres, M., Wang, Z.-Q., Namura, S., Waeber, C., & Moskowitz, M. A. (1997) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1097/00004647-199711000-00002 · Cited by 622
Metadata
- Authors
- Tilly, J. L., & Hsueh, A. J. W.
- Venue
- Journal of Cellular Physiology
- Publisher
- Wiley
- Published
- 1993-03
- Volume
- 154
- Issue
- 3
- Pages
- 519-526
- ISSN
- 0021-9541 (ISSN-L)
- 1097-4652
- OpenAlex
- W1991619717
- Cited by
- 340
- References
- 18
- FWCI
- 9.99
- Type
- journal article
- Language
- en
- Open access
- closed
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