PLGA Nanoparticles for Oral Delivery of Hydrophobic Drugs: Influence of Organic Solvent on Nanoparticle Formation and Release Behavior In Vitro and In Vivo Using Estradiol as a Model Drug
Abstract
The aim of present investigation was to screen different solvents for optimizing nanoparticle preparation in terms of particle size, entrapment efficiency, and finally, release behavior using a model drug estradiol. Nanoparticles were prepared following emulsion-diffusion-evaporation method using didodecyldimethyl ammonium bromide (DMAB) or polyvinyl alcohol (PVA) as stabilizers. Ethyl acetate (EA), acetone (ACE), chloroform (CHL), and dichloromethane (DCM) were used as organic solvents either individually or in combinations. DMAB when used as surfactant led to smaller particle size as compared to PVA irrespective of the solvents and combinations used, but on the other hand, PVA produced particles with higher entrapment when combinations of solvents used. DCM in combination with EA resulted in highest entrapment with both the stabilizers. All the formulations exhibited similar in vitro release profile (Zero order) irrespective of stabilizer (DMAB or PVA) used, however, the average release per day was higher in case of DCM formulations due to greater entrapment. In situ uptake studies suggest that smaller the particle size better is the uptake. The bioavailability from nanoparticles was assessed in male Sprague Dawley (SD) rats at a dose of 1 mg drug/rat. EA/DMAB (size 116.0 +/- 2.6 nm) and DCM:EA 70:30/DMAB (size 253.0 +/- 5.5 nm) showed the release for 9 and 5 days, respectively, whereas EA/PVA (size 279.3 +/- 2.5 nm) released the drug over the periods of 3 days suggesting that particle size has significant role in determining the fate of nanoparticles in vivo. Histopathological examination revealed absence of any inflammatory response with the formulations under the studied period.
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NPs are rapidly internalized mainly by receptor-mediated endocytosis and then disseminated systemically.
“NPs are rapidly internalized mainly by a process that involves receptor-mediated endocytosis, and are then disseminated systemically [5,38].”
From Use of lectin-functionalized particles for oral immunotherapy (2012) · cites this paper as [38]
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- Authors
- Sahana, D. K., Mittal, G., Bhardwaj, V., & Kumar, M. N. V. R.
- Affiliations
- National Institute of Pharmaceutical Education and Research (IN)
- Corresponding author
- Kumar, M.N.V.Ravi
- Venue
- Journal of Pharmaceutical Sciences
- Publisher
- Elsevier BV
- Published
- 2008-04
- Volume
- 97
- Issue
- 4
- Pages
- 1530-1542
- ISSN
- 0022-3549 (ISSN-L)
- OpenAlex
- W1990648838
- Cited by
- 260
- References
- 38
- Citations by year
- 2026: 9 · 2025: 6 · 2024: 6 · 2023: 13 · 2022: 12 · 2021: 14 · 2020: 21 · 2019: 14 · 2018: 18 · 2017: 20 · 2016: 18 · 2015: 27 · 2014: 25 · 2013: 12 · 2012: 16
- FWCI
- 5.18
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Pharmaceutical Sciences (publishedVersion)
- PubMed (publishedVersion)
- Strathprints: The University of Strathclyde institutional repository (University of Strathclyde) (submittedVersion)
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Drug Solubulity and Delivery Systems
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- PLGA nanoparticles, oral drug delivery, poorly water-soluble drugs, estradiol, organic solvent selection, polyvinyl alcohol stabilizer, particle size, encapsulation efficiency, in vitro drug release, bioavailability, nanoparticle toxicity
- MeSH terms
- Polylactic Acid-Polyglycolic Acid Copolymer, Administration, Oral, Animals, Estradiol, Liver, Male, Particle Size, Polyglycolic Acid, Polymers, Solubility, Solvents, Drug Delivery Systems, Rats, Sprague-Dawley, Lactic Acid, Rats, Nanoparticles
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