Supercritical fluid extraction of ecdysterone from the roots of Achyranthes bidentata BL.
Abstract
Ecdysterone has been found in a great many plants and animals and has some valuable pharmaceutical properties. The present study was conducted to investigate optimal conditions for the extraction of the compound by supercritical fluid extraction from the roots of Achyranthes bidentata BL. An orthogonal array design (OAD), OA(9)(3(4)), was employed as a chemometric method for optimization of the extraction of ecdysterone from the herbal medicine. Four parameters, namely, pressure and temperature of the supercritical fluid, the dynamic extraction time, and the flow rate of dimethyl sulfoxide, were studied and optimized by a three-level OAD. Determinations of the extracts were performed by high-performance liquid chromatography. The effects of the parameters were studied using analysis of variance. The results shown that the yield of ecdysterone could be influenced by the four parameters to a similar degree. The yield for DMSO-modified supercritical CO(2) was in the range from 0.65 to 1.03 mg/g under the selected conditions. In comparison with methanol-modified supercritical CO(2 )and Soxhlet extraction, a higher yield was obtained when DMSO-modified supercritical CO(2) was used.
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Supercritical fluid extraction with critical carbon dioxide modified with methanol, ethanol or DMSO is an effective, high-yield method for extracting ecdysteroids from plant materials.
“Supercritical fluid with critical carbon dioxide modified with methanol, ethanol or DMSO is an effective, high-yield extraction method for ecdysteroid-containing plant materials [110-113].”
From Ecdysteroids (2021) · cites this paper as [111]
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- Authors
- Zheng, Y., Liu, B., Chen, M., & Chen, T.
- Affiliations
- NingboTech University (CN)
- Corresponding author
- Liu, Ben
- Venue
- Journal of Separation Science
- Publisher
- Wiley
- Published
- 2008-04-30
- Volume
- 31
- Issue
- 8
- Pages
- 1393-1398
- ISSN
- 1615-9306 (ISSN-L)
- 1615-9314
- OpenAlex
- W2030530959
- Cited by
- 19
- References
- 16
- Citations by year
- 2026: 1 · 2025: 1 · 2021: 1 · 2017: 2 · 2016: 1 · 2014: 3 · 2013: 2
- FWCI
- 1.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Separation Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Phase Equilibria and Thermodynamics
- Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Keywords
- 20-hydroxyecdysone, Achyranthes bidentata, supercritical fluid extraction, roots, extraction optimization, orthogonal experimental design, supercritical carbon dioxide, herbal medicine, extraction yield
- MeSH terms
- Analysis of Variance, Carbon Dioxide, Chemistry Techniques, Analytical, Chromatography, Chromatography, High Pressure Liquid, Ecdysterone, Models, Chemical, Plant Extracts, Plants, Medicinal, Pressure, Temperature, Time Factors, Plant Roots, Chromatography, Supercritical Fluid, Achyranthes
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