Protective effect of ecdysterone on PC12 cells cytotoxicity induced by Beta-amyloid25–35
Abstract
Objective To examine the protective effect of ecdysterone (ECR) against beta-amyloid peptide fragment(25-35) (Abeta(25-35))-induced PC12 cells cytotoxicity, and to further explore its mechanism.Methods Experimental PC12 cells were divided into the Abeta group (treated by Abeta(25-35) 100 micromol/L), the blank group (untreated), the positive control group (treated by Vit E 100 micromol/L after induction) and the ECR treated groups (treated by ECR with different concentrations of 1, 50 and 100 micromol/L). The damaged and survival condition of PC12 cells in various groups was monitored by lactate dehydrogenase (LDH) release and MTT assay. The content of malondialdehyde (MDA) was measured by fluorometric assay to indicate the lipid peroxidation. And the antioxidant enzymes activities in PC12 cells, including superoxide dismutases (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), were detected respectively.Results After PC12 cells were treated with Abeta(25-35) (100 micromol/L) for 24 hrs, they revealed a great decrease in MTT absorbance and activity of antioxidant enzymes, including SOD, CAT and GSH-Px as well as a significant increase of LDH activity and MDA content in PC12 cells (P < 0.01). When the cells was pretreated with 1-100 micromol/L ECR for 24 hrs before Abeta(25-35) treatment, the above-mentioned cytotoxic effect of Abeta(25-35) could be significantly attenuated dose-dependently, for ECR 50 micromol/L, P < 0.05 and for ECR 100 micromol/L, P < 0.01. Moreover, ECR also showed significant inhibition on the Abeta(25-35) induced decrease of SOD and GSH-Px activity, but not on that of CAT.Conclusion ECR could protect PC12 cells from cytotoxicity of Abeta(25-35), and the protective mechanism might be related to the increase of SOD and GSH-Px activities and the decrease of MDA resulting from the ECR-pretreatment.
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Earlier behavioral studies indicated that Ecdy caused shorter latency and searching distance in the Morris water maze test between Aβ-induced rats, meaning Ecdy could improve impaired spatial memory.
“earlier studies in terms of behavioral investigations have indicated that Ecdy caused shorter latency and searching distance in Morris water maze test between Aβ-induced rats that means Ecdy could improve impaired spatial memory (22).”
Cites
McCord, J. M., & Fridovich, I. (1969) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)63504-5 · Cited by 12,952 · see citing works
Lipton, S. A., Choi, Y.-B., Pan, Z.-H., Lei, S. Z., Chen, H.-S. V., Sucher, N. J., Loscalzo, J., Singel, D. J., & Stamler, J. S. (1993) · Nature · doi:10.1038/364626a0 · Cited by 2,433 · see citing works
Hydrogen peroxide mediates amyloid β protein toxicity
Behl, C. (1994) · Cell · doi:10.1016/0092-8674(94)90131-7 · Cited by 2,074 · see citing works
Greenlund, L. J. S., Deckwerth, T. L., & Johnson, E. M. (1995) · Neuron · doi:10.1016/0896-6273(95)90287-2 · Cited by 738 · see citing works
Vitamin E protects nerve cells from amyloid βprotein toxicity
Behl, C., Davis, J. B., Cole, G. M., & Schubert, D. A. (1992) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(92)90837-b · Cited by 582 · see citing works
Amyloid‐β Deposition in Alzheimer Transgenic Mice Is Associated with Oxidative Stress
Smith, M. A., Hirai, K., Hsiao, K., Pappolla, M. A., Harris, P. L. R., Siedlak, S. L., Tabaton, M., & Perry, G. L. (1998) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1998.70052212.x · Cited by 547 · see citing works
Shearman, M. S., RAGAN, C. I., & Iversen, L. L. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.4.1470 · Cited by 411 · see citing works
Butterfield, D. A. (1997) · Chemical Research in Toxicology · doi:10.1021/tx960130e · Cited by 364 · see citing works
Goodman, Y., Steiner, M. R., Steiner, S. M., & Mattson, M. P. (1994) · Brain Research · doi:10.1016/0006-8993(94)91586-5 · Cited by 202 · see citing works
Zhou, Y., Gopalakrishnan, V., & Richardson, J. S. (1996) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1996.67041419.x · Cited by 81 · see citing works
[Antiradical properties and antioxidant activity of ecdysterone].
Lf, O., Lm, S., & Kholodova, I. D. (1992) · PubMed · Cited by 12 · see citing works
Yang, S., Yang, Z., Zhou, Q., Wu, Q., Huang, X.-N., & Shi, J. (2004) · PubMed · Cited by 4 · see citing works
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Kaminsky, Y. G., Marlatt, M. W., Smith, M. A., & Kosenko, E. A. (2009) · Experimental Neurology · doi:10.1016/j.expneurol.2009.09.005 · Cited by 165 · see citing works
Gholipour, P., Komaki, A., Parsa, H., & Ramezani, M. (2022) · Neurochemical Research · doi:10.1007/s11064-022-03603-2 · Cited by 43 · see citing works
Gholipour, P., Komaki, A., Ramezani, M., & Parsa, H. (2022) · Physiology & Behavior · doi:10.1016/j.physbeh.2022.113817 · Cited by 37 · see citing works
Zou, Y., Wang, R., Guo, H., & Dong, M. (2015) · Toxicological Sciences · doi:10.1093/toxsci/kfv111 · Cited by 36 · see citing works
Lin, L.-W., Tsai, F.-H., Lan, W.-C., Cheng, Y.-D., Lee, S.-C., & Wu, C.-R. (2019) · Molecular Neurobiology · doi:10.1007/s12035-018-1436-7 · Cited by 31 · see citing works
Ecdysterone and its Activity on some Degenerative Diseases
Cahlíková, L., Macáková, K., Chlebek, J., Hošťálková, A., Kulhánková, A., & Opletal, L. (2011) · Natural Product Communications · doi:10.1177/1934578x1100600527 · Cited by 26 · see citing works
Supercritical fluid extraction of ecdysterone from the roots of <i>Achyranthes bidentata</i> BL.
Zheng, Y., Liu, B., Chen, M., & Chen, T. (2008) · Journal of Separation Science · doi:10.1002/jssc.200700468 · Cited by 19 · see citing works
Xing, H., Chen, G., Raza, F., Zafar, H., Xing, L., Li, Q., Li, R., Xu, S., & Chen, D. (2024) · Frontiers in Bioscience-Landmark · doi:10.31083/j.fbl2903109 · Cited by 11 · see citing works
Gholipour, P., Komaki, A., Parsa, H., & Ramezani, M. (2025) · Molecular Neurobiology · doi:10.1007/s12035-025-05168-x · Cited by 10 · see citing works
Xing, H., Sun, X., Xing, L., Li, Q., Puyang, M., Liu, L., & Chen, D. (2025) · Chemical Engineering Journal · doi:10.1016/j.cej.2025.167717 · Cited by 3 · see citing works
- Authors
- Su-fen, Y., Zhong-jun, W., Zheng-qin, Y., Qin, W., Qi-hai, G., Qi-xin, Z., & Jing-shan, S.
- Affiliations
- Chongqing Medical University (CN)
- Venue
- Chinese Journal of Integrative Medicine
- Published
- 2005-12
- Volume
- 11
- Issue
- 4
- Pages
- 293-296
- ISSN
- 1672-0415 (ISSN-L)
- 1993-0402
- OpenAlex
- W2326186222
- Cited by
- 10
- References
- 12
- Citations by year
- 2025: 2 · 2024: 1 · 2022: 2 · 2019: 1 · 2015: 1
- FWCI
- 0.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Chinese Journal of Integrative Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Keywords
- 20-hydroxyecdysone, amyloid beta 25-35, PC12 cells, neuroprotection, cytotoxicity, oxidative stress, lipid peroxidation, malondialdehyde, superoxide dismutase, glutathione peroxidase, catalase, antioxidant enzymes, Alzheimer's disease, neurotoxicity, vitamin E, neuroprotection mechanism, neurodegeneration
- MeSH terms
- Animals, Catalase, Ecdysterone, Glutathione Peroxidase, L-Lactate Dehydrogenase, Malondialdehyde, Peptide Fragments, Amyloid beta-Peptides, PC12 Cells, Rats
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