Supercritical fluid extraction of cynaropicrin and 20‐hydroxyecdysone from Leuzea carthamoides DC
Abstract
Leuzea carthamoides is an adaptogenic plant containing biologically active compounds as ecdysteroids and guaianolide-type sesquiterpene lactones, conventionally extracted from the plant with ethanol. It may be a potential source of the mentioned natural compounds. Ethanol-modified near-critical CO(2) was used as selective solvent with the aim to increase the level of 20-hydroxyecdysone in the extract from L. carthamoides roots and to remove selectively cynaropicrin, a sesquiterpene lactone of bitter taste, from the leaves. The extraction conditions were varied (pressure 20-28 MPa, temperature 40-60 degrees C, ethanol concentration in the solvent 0-7.1%) and the extraction yield and extract composition were compared with the results of ethanolic extraction. The supercritical fluid extraction (SFE) from finely powdered plant was controlled by phase equilibrium. Cynaropicrin was quantitatively removed from the leaves where 89% of 20-hydroxyecdysone was retained. The extraction yield of 20-hydroxyecdysone from roots with ethanol-modified CO(2 )was lower by 30% than with ethanol but its concentration in the extract was higher by 67%.
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Supercritical fluid extraction with critical carbon dioxide modified with methanol, ethanol or DMSO is an effective, high-yield method for extracting ecdysteroids from plant materials.
“Supercritical fluid with critical carbon dioxide modified with methanol, ethanol or DMSO is an effective, high-yield extraction method for ecdysteroid-containing plant materials [110-113].”
From Ecdysteroids (2021) · cites this paper as [110]
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- Authors
- Sovová, H., Opletal, L., Sajfrtová, M., & Bártlová, M.
- Affiliations
- Czech Academy of Sciences (CZ)
- Corresponding author
- Sovová, Helena
- Venue
- Journal of Separation Science
- Publisher
- Wiley
- Published
- 2008-04-30
- Volume
- 31
- Issue
- 8
- Pages
- 1387-1392
- ISSN
- 1615-9306 (ISSN-L)
- 1615-9314
- OpenAlex
- W2094679240
- Cited by
- 21
- References
- 23
- Citations by year
- 2024: 1 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 1 · 2013: 2 · 2012: 1
- FWCI
- 1.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Separation Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Phase Equilibria and Thermodynamics
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Physical Sciences › Chemical Engineering › Catalysis › Ionic liquids properties and applications
- Keywords
- Leuzea carthamoides, supercritical fluid extraction, cynaropicrin, 20-hydroxyecdysone, ecdysteroids, selective extraction, plant roots, leaves, extraction yield, phase equilibria
- MeSH terms
- Ethanol, Chemistry Techniques, Analytical, Ecdysterone, Lactones, Models, Chemical, Plant Extracts, Pressure, Sesquiterpenes, Solvents, Temperature, Plant Leaves, Plant Roots, Chromatography, Supercritical Fluid, Leuzea
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