Resveratrol Inhibits Human Visceral Preadipocyte Proliferation and Differentiation in vitro
Abstract
Visceral obesity is a high-risk factor for diabetes and metabolic syndrome. Resveratrol, a natural polyphenolic compound, has been reported to inhibit preadipocyte differentiation. However, the effect of resveratrol on human visceral preadipocyte (HPA-v) differentiation remains largely unknown. LIM domain only 3 (LMO3) promotes human preadipocyte differentiation by enhancing peroxisome proliferator-activated receptor γ (PPARγ) transcriptional activity, which is the master regulator of adipogenesis. The purpose of our study was to determine the effect of resveratrol (0-50 μM) on HPA-v proliferation and differentiation, and the role of LMO3 in resveratrol-mediated regulation of HPA-v differentiation. Resveratrol inhibited HPA-v proliferation and differentiation in a dose-dependent manner, and significantly decreased the mRNA expression levels of PPARG, CCAAT/enhancer-binding protein α (CEBPA), fatty acid-binding protein 4 (FABP4), acetyl-CoA carboxylase (ACC), and fatty acid synthase (FAS) (p < 0.05) at 10, 20, and 50 μM. The mRNA and protein levels of LMO3 were significantly reduced by ≥20 μM resveratrol (p < 0.05), and overexpression of LMO3 partially attenuated resveratrol-induced reduction of HPA-v differentiation by enhancing the PPARG transcriptional activity. Together, our study suggested that resveratrol reduced HPA-v proliferation and differentiation, as well as LMO3, which was partially responsible for the reduction of resveratrol-mediated adipocyte differentiation.
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Resveratrol inhibits adipocyte proliferation.
“In addition, the polyphenol resveratrol was able to inhibit adipocyte proliferation [48,49].”
From Mediterranean Diet as an Antioxidant: The Impact on Metabolic Health and Overall Wellbeing (2021) · cites this paper as [48]
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- Authors
- Sun, W., Yu, S., Han, H., Yuan, Q., Chen, J., & Xu, G.
- Affiliations
- Nantong University (CN)
- Hebei University of Engineering (CN)
- Corresponding author
- Xu, Guangfei
- Venue
- Lipids
- Publisher
- Wiley
- Published
- 2019-10
- Volume
- 54
- Issue
- 11-12
- Pages
- 679-686
- ISSN
- 0024-4201 (ISSN-L)
- 1558-9307
- OpenAlex
- W2977789176
- Cited by
- 11
- References
- 46
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 4
- FWCI
- 0.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Lipids (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81502803, 21377062)
- Topics
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- resveratrol, adipogenesis, cell proliferation, PPARγ, visceral obesity, metabolic syndrome, CEBPA, FABP4, acetyl-CoA carboxylase, fatty acid synthase
- MeSH terms
- Resveratrol, Cell Differentiation, Cell Survival, Cells, Cultured, Dose-Response Relationship, Drug, Humans, Structure-Activity Relationship, Adipocytes, Cell Proliferation
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