Quantitative Gd‐DOTA uptake from cerebrospinal fluid into rat brain using 3D VFA‐SPGR at 9.4T
Open accessAbstract
Purpose: We propose a quantitative technique to assess solute uptake into the brain parenchyma based on dynamic contrast‐enhanced MRI (DCE‐MRI). With this approach, a small molecular weight paramagnetic contrast agent (Gd‐DOTA) is infused in the cerebral spinal fluid (CSF) and whole brain gadolinium concentration maps are derived. Methods: We implemented a 3D variable flip angle spoiled gradient echo (VFA‐SPGR) longitudinal relaxation time (T1) technique, the accuracy of which was cross‐validated by way of inversion recovery rapid acquisition with relaxation enhancement (IR‐RARE) using phantoms. Normal Wistar rats underwent Gd‐DOTA infusion into CSF via the cisterna magna and continuous MRI for approximately 130 min using T1‐weighted imaging. Dynamic Gd‐DOTA concentration maps were calculated and parenchymal uptake was estimated. Results: In the phantom study, T1 discrepancies between the VFA‐SPGR and IR‐RARE sequences were approximately 6% with a transmit coil inhomogeneity correction. In the in vivo study, contrast transport profiles indicated maximal parenchymal retention of approximately 19% relative to the total amount delivered into the cisterna magna. Conclusion: Imaging strategies for accurate 3D contrast concentration mapping at 9.4T were developed and whole brain dynamic concentration maps were derived to study solute transport via the glymphatic system. The newly developed approach will enable future quantitative studies of the glymphatic system in health and disease states. Magn Reson Med 79:1568–1578, 2018. © 2017 International Society for Magnetic Resonance in Medicine.
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In rats, from total amount of MRI contrast agent injected into cisterna magna, merely 19% enriched brain parenchyma, remainder escaped via other routes.
“It has, however, been demonstrated in rats that from the total amount of MRI contrast agent injected into the cisterna magna, merely 19% enriched the brain parenchyma, the remainder escaped via other routes22.”
From Cerebrospinal fluid tracer efflux to parasagittal dura in humans (2020) · cites this paper as [22]
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- Authors
- Lee, H., Mortensen, K., Sanggaard, S., Koch, P., Brunner, H., Quistorff, B., Nedergaard, M., & Benveniste, H.
- Affiliations
- Yale University (US)
- University of Copenhagen (DK)
- Section for Translational Neuroscience Faculty of Health and Medical Sciences, University of Copenhagen Copenhagen Denmark
- Corresponding author
- Lee, Hedok
- Venue
- Magnetic Resonance in Medicine
- Publisher
- Wiley
- Published
- 2017-06-19
- Volume
- 79
- Issue
- 3
- Pages
- 1568-1578
- ISSN
- 0740-3194 (ISSN-L)
- 1522-2594
- PMC
- PMC5736474
- OpenAlex
- W2625314800
- Cited by
- 110
- References
- 55
- Citations by year
- 2026: 7 · 2025: 9 · 2024: 16 · 2023: 11 · 2022: 14 · 2021: 13 · 2020: 17 · 2019: 10 · 2018: 11 · 2017: 2
- FWCI
- 8.40
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Magnetic Resonance in Medicine (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, other-oa)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (NIH‐R01AG048769, NIH 1R01NS100366‐01, NIH‐RF1AG053991)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Physical Sciences › Physics and Astronomy › Nuclear and High Energy Physics › NMR spectroscopy and applications
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Cerebrospinal fluid and hydrocephalus
- Keywords
- Gd-DOTA, cerebrospinal fluid, solute transport, glymphatic system, dynamic contrast-enhanced MRI, T1 mapping, high-field MRI, rat brain
- MeSH terms
- Algorithms, Animals, Brain, Brain Chemistry, Brain Mapping, Heterocyclic Compounds, Image Processing, Computer-Assisted, Magnetic Resonance Imaging, Male, Organometallic Compounds, Rats, Wistar, Phantoms, Imaging, Rats
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