Carbon nanotube rope with electrical stimulation promotes the differentiation and maturity of neural stem cells.
Abstract
In recent years, the utilization of nanomaterials such as carbon nanotubes (CNTs) in the field of neuroscience has forever changed the approach to nerve-related research. The array of novel properties CNTs possess allows them to interact with neurons at the nanodimensional scale. In this study, a CNT rope substrate is developed to allow the electrical stimulation of neural stem cells (NSCs) in culture medium and the in situ observation of the response of these stem cells after stimulation. CNTs are synthesized by chemical vapor deposition and prepared into a ropelike structure with a diameter of 1 mm and length of 1.5 cm. NSCs are differentiated on the CNT rope substrate while the direction of neurite outgrowth, phenotype, and maturity of the NSCs are analyzed. Fluorescence and scanning electron microscopy demonstrate that neurite extension favors the direction of the spiral topography on the CNT rope. NSCs plated on CNT ropes are boosted towards differentiated neurons in the early culture stage when compared to conventional tissue culture plates via the analysis of neuronal gene and protein expressions by quantitative polymerase chain reaction and immunostaining, respectively. Furthermore, a set of electrical stimulation parameters (5 mV, 0.5 mA, 25 ms intermittent stimulation) promotes neuronal maturity while also increasing the speed of neurite outgrowth. These results indicate that an electroconductive CNT rope substrate along with electrical stimulation may have a synergistic effect on promoting neurite elongation and boosting effects on the differentiation of NSCs into mature neuronal cells for therapeutic application in neural regeneration.
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Interaction of carbon nanotubes with stem cells provides new opportunities in nerve tissue engineering.
“The interaction of carbon nanotubes with stem cell provides new opportunities in nerve tissue engineering to explore and add to cell behavior”
From Nanotechnology: A Promising Approach for Delivery of Neuroprotective Drugs (2020) · cites this paper as Huang et al., 2012
Cites
Raman spectroscopy of carbon nanotubes
Dresselhaus, M. S., Dresselhaus, G., Saito, R., & Jório, A. (2005) · Physics Reports · doi:10.1016/j.physrep.2004.10.006 · Cited by 4,509 · see citing works
Ajayan, P. M. (1999) · Chemical Reviews · doi:10.1021/cr970102g · Cited by 3,163 · see citing works
Direct Spinning of Carbon Nanotube Fibers from Chemical Vapor Deposition Synthesis
Li, Y., Kinloch, I. A., & Windle, A. H. (2004) · Science · doi:10.1126/science.1094982 · Cited by 1,486 · see citing works
Lewis, J. D., Meehan, R. R., Henzel, W. J., Maurer-Fogy, I., Jeppesen, P., Klein, F., & Bird, A. (1992) · Cell · doi:10.1016/0092-8674(92)90610-o · Cited by 1,371 · see citing works
Controlling Cell Behavior Electrically: Current Views and Future Potential
McCaig, C., Rajnicek, A. M., Song, B., & Zhao, M. (2005) · Physiological Reviews · doi:10.1152/physrev.00020.2004 · Cited by 1,098 · see citing works
Stimulation of neurite outgrowth using an electrically conducting polymer
Schmidt, C. E., Shastri, V. P., Vacanti, J. P., & Langer, R. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.17.8948 · Cited by 1,072 · see citing works
Carbon nanotube applications for tissue engineering
Harrison, B. S., & Atala, A. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.07.044 · Cited by 1,068 · see citing works
Survival and differentiation of adult neuronal progenitor cells transplanted to the adult brain.
Gage, F. H., Coates, P. W., Palmer, T. D., Kuhn, H. G., Fisher, L. J., Suhonen, J., Peterson, D. A., Suhr, S. T., & Ray, J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.25.11879 · Cited by 1,020 · see citing works
The biocompatibility of carbon nanotubes
Smart, S. K., Cassady, A. I., Lu, G. Q., & Martin, D. J. (2006) · Carbon · doi:10.1016/j.carbon.2005.10.011 · Cited by 1,011 · see citing works
Biomolecule‐Functionalized Carbon Nanotubes: Applications in Nanobioelectronics
Katz, E. Y., & Willner, I. (2004) · ChemPhysChem · doi:10.1002/cphc.200400193 · Cited by 750 · see citing works
Enhanced Differentiation of Human Neural Stem Cells into Neurons on Graphene
Park, S. Y., Park, J., Sim, S. H., Sung, M. G., Kim, K. S., Hong, B. H., & Hong, S. (2011) · Advanced Materials · doi:10.1002/adma.201101503 · Cited by 703 · see citing works
Ghasemi‐Mobarakeh, L., Prabhakaran, M. P., Morshed, M., Nasr‐Esfahani, M. H., Baharvand, H., Kiani, S., Al‐Deyab, S. S., & Ramakrishna, S. (2011) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.383 · Cited by 699 · see citing works
Carbon Nanotube Substrates Boost Neuronal Electrical Signaling
Lovat, V., Pantarotto, D., Lagostena, L., Cacciari, B., Grandolfo, M., Righi, M., Spalluto, G., Prato, M., & Ballerini, L. (2005) · Nano Letters · doi:10.1021/nl050637m · Cited by 648 · see citing works
Camilli, P. V. D., Cameron, R. S., & Greengard, P. (1983) · The Journal of Cell Biology · doi:10.1083/jcb.96.5.1337 · Cited by 562 · see citing works
Toxicity issues in the application of carbon nanotubes to biological systems
Firme, C. P., & Bandaru, P. R. (2009) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2009.07.003 · Cited by 544 · see citing works
Multiwall carbon nanotube scaffolds for tissue engineering purposes
Abarrategi, A., Gutiérrez, M. C., Moreno-Vicente, C., Hortigüela, M. J., Ramos, V., López‐Lacomba, J. L., Ferrer, M. L., & del Monte, F. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.09.021 · Cited by 436 · see citing works
Electrical stimulation promotes sensory neuron regeneration and growth-associated gene expression
Geremia, N. M., Gordon, T., Brushart, T. M., Al-Majed, A. A., & Verge, V. M. K. (2007) · Experimental Neurology · doi:10.1016/j.expneurol.2007.01.040 · Cited by 430 · see citing works
Ando, Y., Zhao, X., Sugai, T., & Kumar, M. (2004) · Materials Today · doi:10.1016/s1369-7021(04)00446-8 · Cited by 234 · see citing works
The tubulin fraternity: alpha to eta
Dutcher, S. K. (2001) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(00)00173-3 · Cited by 224 · see citing works
Kam, N. W. S., Jan, E., & Kotov, N. A. (2008) · Nano Letters · doi:10.1021/nl802859a · Cited by 208 · see citing works
Carbon nanotubes promote neuron differentiation from human embryonic stem cells
Chao, T.-I., Xiang, S., Chen, C., Chin, W., Nelson, A. J. R., Wang, C., & Lu, J. Q. (2009) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2009.04.157 · Cited by 205 · see citing works
Conductive Single-Walled Carbon Nanotube Substrates Modulate Neuronal Growth
Malarkey, E. B., Fisher, K. A., Bekyarova, E. B., Liu, W., Haddon, R. C., & Parpura, V. (2008) · Nano Letters · doi:10.1021/nl802855c · Cited by 186 · see citing works
Electrotherapy: Evidence-Based Practice
Kitchen, S. S. (2001) · Medical Entomology and Zoology · Cited by 180 · see citing works
Regulation of intrinsic neuronal properties for axon growth and regeneration
Rossi, F., Gianola, S., & Corvetti, L. (2006) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2006.12.001 · Cited by 153 · see citing works
Chang, K.-A., Kim, J. W., Kim, J. A., Lee, S., Kim, S., Suh, W. H., Kim, H.-S., Kwon, S., Kim, S. J., & Suh, Y. (2011) · PLoS ONE · doi:10.1371/journal.pone.0018738 · Cited by 134 · see citing works
The Regulative Role of Neurite Mechanical Tension in Network Development
Anava, S., Greenbaum, A., Jacob, E. B., Hanein, Y., & Ayali, A. (2009) · Biophysical Journal · doi:10.1016/j.bpj.2008.10.058 · Cited by 128 · see citing works
Electrical fields, nerve growth and nerve regeneration
McCaig, C. D., & Rajnicek, A. (1991) · Experimental Physiology · doi:10.1113/expphysiol.1991.sp003514 · Cited by 104 · see citing works
Molecular evolution of type VI intermediate filament proteins
Guérette, D., Khan, P. A., Savard, P. E., & Vincent, M. (2007) · BMC Evolutionary Biology · doi:10.1186/1471-2148-7-164 · Cited by 95 · see citing works
Making sense of the multiple MAP-2 transcripts and their role in the neuron
Shafit-Zagardo, B., & Kalcheva, N. (1998) · Molecular Neurobiology · doi:10.1007/bf02740642 · Cited by 89 · see citing works
Complete structure of the human gene encoding neuron-specific enolase
Oliva, D., Calı̀, L., Feo, S., & Giallongo, A. (1991) · Genomics · doi:10.1016/0888-7543(91)90496-2 · Cited by 56 · see citing works
Interactions Between Cultured Neurons and Carbon Nanotubes: A Nanoneuroscience Vignette
Sucapane, A., Cellot, G., Prato, M., Giugliano, M., Parpura, V., & Ballerini, L. (2009) · Journal of Nanoneuroscience · doi:10.1166/jns.2009.002 · Cited by 53 · see citing works
Electrically Mediated Neuronal Guidance with Applied Alternating Current Electric Fields
Graves, M. S., Hassell, T. J. W., Beier, B. L., Albors, G. O., & Irazoqui, P. P. (2011) · Annals of Biomedical Engineering · doi:10.1007/s10439-011-0259-8 · Cited by 31 · see citing works
Stem cell niches and endogenous electric fields in tissue repair
Li, L., & Jiang, J. (2011) · Frontiers of Medicine · doi:10.1007/s11684-011-0108-z · Cited by 24 · see citing works
Wiring neurons with carbon nanotubes
Lee (2009) · Frontiers in Neuroengineering · doi:10.3389/neuro.16.008.2009 · Cited by 16 · see citing works
Structure and energetics of carbon nanotube ropes
Liu, Z., & Qin, L. (2005) · Carbon · doi:10.1016/j.carbon.2005.03.027 · Cited by 13 · see citing works
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Three-dimensional graphene foam as a biocompatible and conductive scaffold for neural stem cells
Li, N., Zhang, Q., Gao, S. W., Song, Q., Huang, R., Wang, L., Liu, L., Dai, J., Tang, M., & Cheng, G. (2013) · Scientific Reports · doi:10.1038/srep01604 · Cited by 659 · see citing works
Thrivikraman, G., Boda, S. K., & Basu, B. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.10.003 · Cited by 386 · see citing works
Nanotechnology: A Promising Approach for Delivery of Neuroprotective Drugs
Naqvi, S., Panghal, A., & Flora, S. J. S. (2020) · Frontiers in Neuroscience · doi:10.3389/fnins.2020.00494 · Cited by 305 · see citing works
Polymer-based composites by electrospinning: Preparation & functionalization with nanocarbons
Lee, J. K. Y., Chen, N., Peng, S., Li, L., Tian, L., Thakor, N. V., & Ramakrishna, S. (2018) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2018.07.002 · Cited by 284 · see citing works
Carbon‐Based Nanomaterials for Tissue Engineering
Ku, S. H., Lee, M., & Park, C. B. (2012) · Advanced Healthcare Materials · doi:10.1002/adhm.201200307 · Cited by 271 · see citing works
3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration
Lee, S., Zhu, W., Nowicki, M. A., Lee, G., Heo, D. N., Kim, J., Zuo, Y. Y., & Zhang, L. G. (2017) · Journal of Neural Engineering · doi:10.1088/1741-2552/aa95a5 · Cited by 238 · see citing works
Three-Dimensional Printing of Nanomaterial Scaffolds for Complex Tissue Regeneration
O'Brien, C. M., Holmes, B. B., Faucett, S. C., & Zhang, L. G. (2014) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2014.0168 · Cited by 201 · see citing works
Biomaterials and cells for neural tissue engineering: Current choices
Sensharma, P., Madhumathi, G., Jayant, R. D., & Jaiswal, A. K. (2017) · Materials Science and Engineering C · doi:10.1016/j.msec.2017.03.264 · Cited by 194 · see citing works
Electrical stimulation affects neural stem cell fate and function in vitro
Zhu, R., Sun, Z., Li, C., Ramakrishna, S., Chiu, K., & He, L. (2019) · Experimental Neurology · doi:10.1016/j.expneurol.2019.112963 · Cited by 191 · see citing works
Ferrigno, B. W., Bordett, R., Duraisamy, N., Moskow, J., Arul, M. R., Rudraiah, S., Nukavarapu, S. P., Vella, A. T., & Kumbar, S. G. (2020) · Bioactive Materials · doi:10.1016/j.bioactmat.2020.03.010 · Cited by 188 · see citing works
Are Carbon Nanotubes a Natural Solution? Applications in Biology and Medicine
Heister, E., Brunner, E. W., Dieckmann, G. R., Jurewicz, I., & Dalton, A. Β. (2013) · ACS Applied Materials & Interfaces · doi:10.1021/am302902d · Cited by 181 · see citing works
Carbon nanotubes in neuroregeneration and repair
Fabbro, A., Prato, M., & Ballerini, L. (2013) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2013.07.002 · Cited by 173 · see citing works
Graphene‐Based Materials in Regenerative Medicine
Ding, X., Liu, H., & Fan, Y. (2015) · Advanced Healthcare Materials · doi:10.1002/adhm.201500203 · Cited by 172 · see citing works
Carbon nanotubes leading the way forward in new generation 3D tissue engineering
Hopley, E. L., Salmasi, S., Kalaskar, D. M., & Seifalian, A. M. (2014) · Biotechnology Advances · doi:10.1016/j.biotechadv.2014.05.003 · Cited by 167 · see citing works
Yang, C., Huang, W., Chen, L.-H., Liang, N., Wang, H.-C., Lü, J., Wang, X., & Wang, T. (2020) · Journal of Materials Chemistry B · doi:10.1039/d0tb01605e · Cited by 162 · see citing works
Guo, R., Xiao, M., Zhao, W., Zhou, S., Hu, Y., Liao, M., Wang, S., Yang, X., Chai, R., & Tang, M. (2020) · Acta Biomaterialia · doi:10.1016/j.actbio.2020.12.035 · Cited by 150 · see citing works
Qian, Y., Yuan, W., Cheng, Y. G., Yang, Y., Qu, X., & Fan, C. (2019) · Nano Letters · doi:10.1021/acs.nanolett.9b03980 · Cited by 145 · see citing works
Koppes, A. N., Keating, K. W., McGregor, A. L., Koppes, R. A., Kearns, K. R., Ziemba, A. M., McKay, C. A., Zuidema, J. M., Rivet, C. J., Gilbert, R. J., et al. (2016) · Acta Biomaterialia · doi:10.1016/j.actbio.2016.05.014 · Cited by 141 · see citing works
Wu, C., Liu, A., Chen, S., Zhang, X., Chen, L., Zhu, Y., Xiao, Z., Sun, J., Luo, H., & Fan, H. (2019) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.9b05520 · Cited by 132 · see citing works
Carbon-nanotube-interfaced glass fiber scaffold for regeneration of transected sciatic nerve
Ahn, H.-S., Hwang, J., Kim, M. S., Lee, J.-Y., Kim, J., Kim, H.-S., Shin, U. S., Knowles, J. C., Kim, H., & Hyun, J. K. (2014) · Acta Biomaterialia · doi:10.1016/j.actbio.2014.11.026 · Cited by 122 · see citing works
Zhang, B., Yan, W., Zhu, Y., Yang, W., Le, W., Chen, B., Zhu, R., & Cheng, L. (2018) · Advanced Materials · doi:10.1002/adma.201705694 · Cited by 113 · see citing works
Regulation of stem cell fate using nanostructure-mediated physical signals
Kong, Y., Duan, J., Liu, F., Han, L. G., Li, G., Sun, C., Sang, Y., Wang, S., Yi, F., & Liu, H. (2021) · Chemical Society Reviews · doi:10.1039/d1cs00572c · Cited by 112 · see citing works
Zhang, S., Ma, B., Liu, F., Duan, J., Wang, S., Qiu, J., Li, D., Sang, Y., Liu, C., Liu, D., et al. (2018) · Nano Letters · doi:10.1021/acs.nanolett.7b04747 · Cited by 110 · see citing works
Liu, X., George, M. N., Park, S., Miller, A. L., Gaihre, B., Li, L., Waletzki, B. E., Terzic, A., Yaszemski, M. J., & Lu, L. (2020) · Acta Biomaterialia · doi:10.1016/j.actbio.2020.04.047 · Cited by 109 · see citing works
Electrical Stimuli in the Central Nervous System Microenvironment
Thompson, D. M., Koppes, A. N., Hardy, J. G., & Schmidt, C. E. (2014) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-121813-120655 · Cited by 107 · see citing works
Zhou, Z., Liu, X., Wu, W., Park, S., Miller, A. L., Terzic, A., & Lu, L. (2018) · Biomaterials Science · doi:10.1039/c8bm00553b · Cited by 93 · see citing works
Yang, K., Yu, S. J., Lee, J. S., Lee, H.-R., Chang, G.-E., Seo, J., Lee, T., Cheong, E., Im, S. G., & Cho, S. (2017) · Nanoscale · doi:10.1039/c7nr05446g · Cited by 90 · see citing works
Role of polymers in the design of 3D carbon nanotube-based scaffolds for biomedical applications
Serrano, M. C., Gutiérrez, M. C., & del Monte, F. (2014) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2014.02.004 · Cited by 91 · see citing works
Shao, H., Li, T., Zhu, R., Xu, X., Yu, J., Chen, S., Song, L., Ramakrishna, S., Lei, Z., Ruan, Y., et al. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.05.028 · Cited by 90 · see citing works
Nanotechnology in peripheral nerve repair and reconstruction
Carvalho, C. R., Silva‐Correia, J., Oliveira, J. M., & Reis, R. L. (2019) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2019.01.006 · Cited by 85 · see citing works
Review: Bioengineering approach for the repair and regeneration of peripheral nerve
Moskow, J., Ferrigno, B. W., Mistry, N., Jaiswal, D., Bulsara, K. R., Rudraiah, S., & Kumbar, S. G. (2018) · Bioactive Materials · doi:10.1016/j.bioactmat.2018.09.001 · Cited by 84 · see citing works
Electroactive nanomaterials in the peripheral nerve regeneration
Yao, X., Qian, Y., & Fan, C. (2021) · Journal of Materials Chemistry B · doi:10.1039/d1tb00686j · Cited by 80 · see citing works
He, L., Sun, Z., Li, J., Zhu, R., Niu, B., Tam, K. L., Xiao, Q., Li, J., Wang, W., Tsui, C.-Y., et al. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120585 · Cited by 77 · see citing works
Advances in Electrical and Magnetic Stimulation on Nerve Regeneration
Qian, Y., Cheng, Y. G., Cai, J., Zhao, X., Ouyang, Y., Yuan, W., & Fan, C. (2019) · Regenerative Medicine · doi:10.2217/rme-2018-0079 · Cited by 78 · see citing works
Zhang, K., Li, J., Jin, J., Dong, J. P., Li, L., Xue, B., Wang, W., Jiang, Q. Y., & Cao, Y. (2020) · Materials & Design · doi:10.1016/j.matdes.2020.109092 · Cited by 75 · see citing works
Liu, X., Kim, J. C., Miller, A. L., Waletzki, B. E., & Lu, L. (2018) · New Journal of Chemistry · doi:10.1039/c8nj03038c · Cited by 74 · see citing works
Wang, J., Li, X., Song, Y., Su, Q., Xiaohalati, X., Yang, W., Xu, L., Cai, B., Wang, G., Wang, Z., et al. (2020) · Bioactive Materials · doi:10.1016/j.bioactmat.2020.12.017 · Cited by 74 · see citing works
Selective neuronal differentiation of neural stem cells induced by nanosecond microplasma agitation
Xiong, Z., Zhao, S., Mao, X., Lu, X., He, G., Yang, G., Chen, M., Ishaq, M., & Ostrikov, K. (2013) · Stem Cell Research · doi:10.1016/j.scr.2013.11.003 · Cited by 73 · see citing works
Neural responses to electrical stimulation in 2D and 3D in vitro environments
Bertucci, C., Koppes, R. A., Dumont, C. M., & Koppes, A. N. (2019) · Brain Research Bulletin · doi:10.1016/j.brainresbull.2019.07.016 · Cited by 69 · see citing works
Farokhi, M., Mottaghitalab, F., Saeb, M. R., Shojaei, S., Zarrin, N. K., Thomas, S., & Ramakrishna, S. (2020) · Macromolecular Bioscience · doi:10.1002/mabi.202000123 · Cited by 70 · see citing works
Utilizing stem cells for three-dimensional neural tissue engineering
Knowlton, S. M., Cho, Y. K., Li, X. J., Khademhosseini, A., & Taşoğlu, S. (2016) · Biomaterials Science · doi:10.1039/c5bm00324e · Cited by 69 · see citing works
Hsu, C., Serio, A., Amdursky, N., Besnard, C., & Stevens, M. M. (2018) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.7b18179 · Cited by 66 · see citing works
Sordini, L., Garrudo, F. F. F., Rodrigues, C. A. V., Linhardt, R. J., Cabral, J. M. S., Ferreira, F. C., & Morgado, J. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.591838 · Cited by 66 · see citing works
A novel electrospun nerve conduit enhanced by carbon nanotubes for peripheral nerve regeneration
Yu, W., Jiang, X., Cai, M., Zhao, W. H., Ye, D., Zhou, Y., Zhu, C., Zhang, X., Lu, X., & Zhang, Z. (2014) · Nanotechnology · doi:10.1088/0957-4484/25/16/165102 · Cited by 66 · see citing works
Towards the translation of electroconductive organic materials for regeneration of neural tissues
Manousiouthakis, E., Park, J., Hardy, J. G., Lee, J. Y., & Schmidt, C. E. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2021.07.065 · Cited by 65 · see citing works
Imaninezhad, M., Pemberton, K., Xu, F., Kalinowski, K., Bera, R., & Zustiak, S. P. (2018) · Journal of Neural Engineering · doi:10.1088/1741-2552/aad65b · Cited by 62 · see citing works
Chondroitin sulphate-based 3D scaffolds containing MWCNTs for nervous tissue repair
Serrano, M. C., Nardecchia, S., García‐Rama, C., Ferrer, M. L., Collazos-Castro, J. E., del Monte, F., & Gutiérrez, M. C. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.11.017 · Cited by 63 · see citing works
Electrical Stimulation Elicits Neural Stem Cells Activation: New Perspectives in CNS Repair
Huang, Y. H., Li, Y., Chen, J., Zhou, H., & Tan, S. H. D. (2015) · Frontiers in Human Neuroscience · doi:10.3389/fnhum.2015.00586 · Cited by 60 · see citing works
Rational design of electrically conductive biomaterials toward excitable tissues regeneration
Zhao, G., Zhou, H., Jin, G., Jin, B., Geng, S., Luo, Z., Ge, Z., & Xu, F. (2022) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2022.101573 · Cited by 61 · see citing works
Sun, Y., Liu, X., George, M. N., Park, S., Gaihre, B., Terzic, A., & Lu, L. (2020) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.37016 · Cited by 61 · see citing works
Three Potential Elements of Developing Nerve Guidance Conduit for Peripheral Nerve Regeneration
Zhang, C., Gong, J., Zhang, J., Zhu, Z., Qian, Y., Lu, K., Zhou, S., Gu, T., Wang, H., He, Y., et al. (2023) · Advanced Functional Materials · doi:10.1002/adfm.202302251 · Cited by 59 · see citing works
Li, J., Zhang, L., Yu, L., Minami, I., Miyagawa, S., Hörning, M., Dong, J., Qiao, J., Qu, X., Hua, Y., et al. (2020) · Communications Biology · doi:10.1038/s42003-020-0853-0 · Cited by 59 · see citing works
Theranostic implications of nanotechnology in multiple sclerosis: a future perspective.
Singh, A. V., Khare, M., Gade, W. N., & Zamboni, P. (2012) · Autoimmune Diseases · doi:10.1155/2012/160830 · Cited by 58 · see citing works
Approaches for Neural Tissue Regeneration
Binan, L., Ajji, A., Crescenzo, G. D., & Jolicœur, M. (2013) · Stem Cell Reviews and Reports · doi:10.1007/s12015-013-9474-z · Cited by 56 · see citing works
Repairing Peripheral Nerves: Is there a Role for Carbon Nanotubes?
Oprych, K. M., Whitby, R. L. D., Mikhalovsky, S. V., Tomlins, P., & Adu, J. (2016) · Advanced Healthcare Materials · doi:10.1002/adhm.201500864 · Cited by 56 · see citing works
Bierman, R. D., Safarians, G., Huang, J., Rajput, B., Chen, J. Y., Wang, Z. Z., & Seidlits, S. K. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202101577 · Cited by 57 · see citing works
Enabling nanomaterial, nanofabrication and cellular technologies for nanoneuromedicines
Mallapragada, S. K., Brenza, T. M., McMillan, J., Narasimhan, B., Sakaguchi, D. S., Sharma, A. D., Zbarska, S., & Gendelman, H. E. (2015) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2014.12.013 · Cited by 54 · see citing works
Conductive hydrogel/nanowire micropattern-based sensor for neural stem cell differentiation
Lee, J. M., Moon, J. Y., Kim, T. H., Lee, S. W., Ahrberg, C. D., & Chung, B. G. (2017) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2017.11.151 · Cited by 54 · see citing works
Ascl1 Regulates Electric Field-Induced Neuronal Differentiation Through PI3K/Akt Pathway
Dong, Z.-Y., Pei, Z., Wang, Y., Li, Z., Khan, A., & Meng, X. (2019) · Neuroscience · doi:10.1016/j.neuroscience.2019.02.004 · Cited by 53 · see citing works
Yildirimer, L., Zhang, Q., Kuang, S., Cheung, J. C., Chu, K. A., He, Y., Yang, M., & Zhao, X. (2019) · Biofabrication · doi:10.1088/1758-5090/ab17aa · Cited by 53 · see citing works
Mechanoregulation of Stem Cell Fate Via Micro-/Nano-Scale Manipulation for Regenerative Medicine
Tay, C. Y., Koh, C.-G., Tan, N. S., Leong, D. T., & Tan, L. P. (2013) · Nanomedicine · doi:10.2217/nnm.13.31 · Cited by 51 · see citing works
Electrospun hyaluronic acid-carbon nanotube nanofibers for neural engineering
Steel, E. M., Azar, J.-Y., & Sundararaghavan, H. G. (2019) · Materialia · doi:10.1016/j.mtla.2019.100581 · Cited by 48 · see citing works
Piezotronic effect determined neuron-like differentiation of adult stem cells driven by ultrasound
Liang, L., Sun, C., Zhang, R., Han, S., Wang, J., Ren, N., & Liu, H. (2021) · Nano Energy · doi:10.1016/j.nanoen.2021.106634 · Cited by 47 · see citing works
Controlling properties of human neural progenitor cells using 2D and 3D conductive polymer scaffolds
Song, S., Amores, D., Chen, C., McConnell, K. W., Oh, B., Poon, A. S. Y., & George, P. M. (2019) · Scientific Reports · doi:10.1038/s41598-019-56021-w · Cited by 48 · see citing works
Tissue Response to Deep Brain Stimulation and Microlesion: A Comparative Study
Vedam‐Mai, V., Baradaran-Shoraka, M., Reynolds, B. A., & Okun, M. S. (2016) · Neuromodulation Technology at the Neural Interface · doi:10.1111/ner.12406 · Cited by 48 · see citing works
Neural responses to electrical stimulation on patterned silk films
Hronik‐Tupaj, M., Raja, W. K., Tang‐Schomer, M. D., Omenetto, F. G., & Kaplan, D. L. (2013) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.34565 · Cited by 46 · see citing works
Electroconductive materials as biomimetic platforms for tissue regeneration
Hosoyama, K., Ahumada, M., Goël, K., Ruel, M., Suuronen, E. J., & Alarcon, E. I. (2019) · Biotechnology Advances · doi:10.1016/j.biotechadv.2019.02.011 · Cited by 47 · see citing works
Ma, X., Luan, Z., & Li, J. (2023) · International Journal of Nanomedicine · doi:10.2147/ijn.s384343 · Cited by 45 · see citing works
Landers, J., Turner, J. T., Heden, G. J., Carlson, A. L., Bennett, N. K., Moghe, P. V., & Neimark, A. V. (2014) · Advanced Healthcare Materials · doi:10.1002/adhm.201400042 · Cited by 42 · see citing works
Suh, T. C., Twiddy, J. S., Mahmood, N., Ali, K. M., Lubna, M. M., Bradford, P. D., Daniele, M. A.-A., & Gluck, J. M. (2022) · ACS Omega · doi:10.1021/acsomega.2c01807 · Cited by 41 · see citing works
Electrical Stimulation of Cells: Drivers, Technology, and Effects
Kadan-Jamal, K., Wronowski, F., Jin, A. T., Naegele, T. E., Montes, V. R., Tao, X., Dominguez‐Alfaro, A., Lee, C., & Malliaras, G. G. (2025) · Chemical Reviews · doi:10.1021/acs.chemrev.4c00468 · Cited by 38 · see citing works
Tissue engineering of the retina: from organoids to microfluidic chips
Marcos, L. F., Wilson, S. L., & Roach, P. (2021) · Journal of Tissue Engineering · doi:10.1177/20417314211059876 · Cited by 33 · see citing works
Liang, L., Liu, C., Cai, P., Han, S., Zhang, R., Ren, N., Wang, J., Yu, J., Shang, S., Zhou, W., et al. (2022) · Nano Energy · doi:10.1016/j.nanoen.2022.107483 · Cited by 34 · see citing works
Platinum and palladium on carbon nanotubes: Experimental and theoretical studies
Adjizian, J.-J., Marco, P. D., Suarez‐Martinez, I., Mel, A. E., Snyders, R., Gengler, R. Y. N., Rudolf, P., Ke, X., TENDELOO, G. V., Bittencourt, C., et al. (2013) · Chemical Physics Letters · doi:10.1016/j.cplett.2013.03.079 · Cited by 31 · see citing works
Zhang, Q., Xu, J., Song, Q., Li, N., Zhang, Z., Li, K., Du, Y., Wu, L., Tang, M., Liu, L., et al. (2014) · Journal of Materials Chemistry B · doi:10.1039/c4tb00279b · Cited by 31 · see citing works
Zhou, J., Cheng, L., Sun, X., Wang, X., Jin, S., Li, J., & Wu, Q. (2016) · Frontiers of Materials Science · doi:10.1007/s11706-016-0348-6 · Cited by 30 · see citing works
Ghorbani, S., Tiraihi, T., & Soleimani, M. (2017) · Journal of Biomaterials Applications · doi:10.1177/0885328217741903 · Cited by 30 · see citing works
Carbon Nanotubes and Their Polymer Nanocomposites
Moses, J. C., Gangrade, A., & Mandal, B. B. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-814615-6.00005-9 · Cited by 28 · see citing works
Electroactive biocompatible materials for nerve cell stimulation
Yang, M., Liang, Y., Gui, Q., Chen, J., & Liu, Y. (2015) · Materials Research Express · doi:10.1088/2053-1591/2/4/042001 · Cited by 28 · see citing works
Jalili, R., Kanneganti, A., Romero‐Ortega, M. I., & Wallace, G. G. (2017) · Current Opinion in Electrochemistry · doi:10.1016/j.coelec.2017.07.003 · Cited by 28 · see citing works
Feng, L., Hao, Y., Zhu, M., Zhai, Y., Yang, L., Liu, Y., & Cheng, G. (2019) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.9b00752 · Cited by 27 · see citing works
Geometry–Force Control of Stem Cell Fate
Worley, K. E., Certo, A., & Wan, L. Q. (2012) · BioNanoScience · doi:10.1007/s12668-012-0067-0 · Cited by 26 · see citing works
Progress in Research of Flexible MEMS Microelectrodes for Neural Interface
Tang, L., Wang, M., Tian, H., Kang, X., Hong, W., & Liu, J. (2017) · Micromachines · doi:10.3390/mi8090281 · Cited by 23 · see citing works
Cesano, F., Uddin, M. J., Damin, A., & Scarano, D. (2021) · Nanomaterials · doi:10.3390/nano11030604 · Cited by 24 · see citing works
Seiti, M., Ginestra, P. S., Ferraro, R. M., Ceretti, E., & Ferraris, E. (2020) · Biofabrication · doi:10.1088/1758-5090/ab71e0 · Cited by 23 · see citing works
Wang, Q., Zhang, J., Yao, G., Lou, W., Zhang, T., Zhang, Z., Xie, M., Gan, X., Pan, T., Gao, M., et al. (2023) · ACS Nano · doi:10.1021/acsnano.3c03385 · Cited by 23 · see citing works
Biomimicry in 3D Printing Design: Implications for Peripheral Nerve Regeneration
Yan, Z., Qian, Y., & Fan, C. (2021) · Regenerative Medicine · doi:10.2217/rme-2020-0182 · Cited by 22 · see citing works
Realisation and characterization of conductive hollow fibers for neuronal tissue engineering
Gattazzo, F., Maria, C. D., Whulanza, Y., Taverni, G., Ahluwalia, A. D., & Vozzi, G. (2014) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.33297 · Cited by 21 · see citing works
Development of plasma-on-chip: Plasma treatment for individual cells cultured in media
Kumagai, S., Chang, C.-Y., Jeong, J., Kobayashi, M., Shimizu, T., & Sasaki, M. (2015) · Japanese Journal of Applied Physics · doi:10.7567/jjap.55.01af01 · Cited by 21 · see citing works
Fraczek–Szczypta, A., Jantas, D., Ciepiela, F., & Grzonka, J. (2019) · Diamond and Related Materials · doi:10.1016/j.diamond.2019.107663 · Cited by 20 · see citing works
Wu, F., Jin, L., Zheng, X., Yan, B., Tang, P., Yang, H., Deng, W., & Yang, W. (2017) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.7b12854 · Cited by 20 · see citing works
Kwan, R. L., Lu, S., Choi, H., Kloth, L. C., & Cheing, G. L. Y. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20020368 · Cited by 19 · see citing works
Physiological Electric Field: A Potential Construction Regulator of Human Brain Organoids
Yu, X., Meng, X., Pei, Z., Wang, G., Liu, R., Qi, M., Zhou, J., & Wang, F. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23073877 · Cited by 18 · see citing works
Zhang, Y., Zhang, M., Zhang, R., Liu, H., Chen, H., Zhang, X., Li, C., Zeng, Q., Chen, Y., & Huang, G. (2022) · Frontiers in Materials · doi:10.3389/fmats.2022.914994 · Cited by 19 · see citing works
Novel Electrode Designs for Neurostimulation in Regenerative Medicine: Activation of Stem Cells
Iwasa, S. N., Shi, H., Hong, S., Chen, T., Marquez-Chin, M., Iorio-Morin, C., Kalia, S., Popovic, M., Naguib, H. E., & Morshead, C. (2020) · Bioelectricity · doi:10.1089/bioe.2020.0034 · Cited by 18 · see citing works
Zieliński, A., & Majkowska-Marzec, B. (2022) · Coatings · doi:10.3390/coatings12111643 · Cited by 18 · see citing works
Rowland, N. C., Kalia, S. K., Kalia, L. V., Larson, P., Lim, D. A., & Bankiewicz, K. S. (2016) · Molecular Therapy — Methods & Clinical Development · doi:10.1038/mtm.2015.51 · Cited by 18 · see citing works
Ma, X., Xiao, M., Hao, Y., & Cheng, G. (2019) · Carbon · doi:10.1016/j.carbon.2019.01.006 · Cited by 18 · see citing works
Panghal, A., & Flora, S. J. S. (2024) · Discover Nano · doi:10.1186/s11671-024-04135-0 · Cited by 19 · see citing works
Züger, F., Marsano, A., Poggio, M., & Gullo, M. R. (2021) · Advanced NanoBiomed Research · doi:10.1002/anbr.202100108 · Cited by 17 · see citing works
- Authors
- Huang, Y., Wu, H., Tai, N., & Wang, T.
- Affiliations
- National Tsing Hua University (TW)
- Tatung University (TW)
- Corresponding author
- Wang, Tzu‐Wei
- Venue
- Small
- Publisher
- Wiley
- Published
- 2012-07-02
- Volume
- 8
- Issue
- 18
- Pages
- 2869-2877
- ISSN
- 1613-6810 (ISSN-L)
- 1613-6829
- OpenAlex
- W2118948952
- Cited by
- 149
- References
- 35
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 5 · 2023: 6 · 2022: 11 · 2021: 14 · 2020: 16 · 2019: 16 · 2018: 14 · 2017: 15 · 2016: 12 · 2015: 6 · 2014: 13 · 2013: 13 · 2012: 3
- FWCI
- 4.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Small (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Council
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- neural stem cells, electrical stimulation, neuronal differentiation, neurite outgrowth, neuronal maturation, substrate topography, nerve regeneration, chemical vapor deposition
- MeSH terms
- Animals, Cell Differentiation, Electric Stimulation, Microscopy, Electron, Scanning, Neurites, Nanotubes, Carbon, Rats, Neural Stem Cells
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