Cited by 149Neuroscience and Neural EngineeringElectrospun Nanofibers in Biomedical ApplicationsTissue Engineering and Regenerative Medicine

Carbon nanotube rope with electrical stimulation promotes the differentiation and maturity of neural stem cells.

Huang, Yu‐Jie, Wu, Hsi‐Chin, Tai, Nyan‐Hwa, Wang, Tzu‐Wei·2012·Small

Abstract

In recent years, the utilization of nanomaterials such as carbon nanotubes (CNTs) in the field of neuroscience has forever changed the approach to nerve-related research. The array of novel properties CNTs possess allows them to interact with neurons at the nanodimensional scale. In this study, a CNT rope substrate is developed to allow the electrical stimulation of neural stem cells (NSCs) in culture medium and the in situ observation of the response of these stem cells after stimulation. CNTs are synthesized by chemical vapor deposition and prepared into a ropelike structure with a diameter of 1 mm and length of 1.5 cm. NSCs are differentiated on the CNT rope substrate while the direction of neurite outgrowth, phenotype, and maturity of the NSCs are analyzed. Fluorescence and scanning electron microscopy demonstrate that neurite extension favors the direction of the spiral topography on the CNT rope. NSCs plated on CNT ropes are boosted towards differentiated neurons in the early culture stage when compared to conventional tissue culture plates via the analysis of neuronal gene and protein expressions by quantitative polymerase chain reaction and immunostaining, respectively. Furthermore, a set of electrical stimulation parameters (5 mV, 0.5 mA, 25 ms intermittent stimulation) promotes neuronal maturity while also increasing the speed of neurite outgrowth. These results indicate that an electroconductive CNT rope substrate along with electrical stimulation may have a synergistic effect on promoting neurite elongation and boosting effects on the differentiation of NSCs into mature neuronal cells for therapeutic application in neural regeneration.

Integrity signals

Registry

Crossref · 10.1002/smll.201200715

Cites

35 of 35 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Interaction of carbon nanotubes with stem cells provides new opportunities in nerve tissue engineering.

    “The interaction of carbon nanotubes with stem cell provides new opportunities in nerve tissue engineering to explore and add to cell behavior”

    From Nanotechnology: A Promising Approach for Delivery of Neuroprotective Drugs (2020) · cites this paper as Huang et al., 2012

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Huang, Y., Wu, H., Tai, N., & Wang, T.
Affiliations
  • National Tsing Hua University (TW)
  • Tatung University (TW)
Corresponding author
Wang, Tzu‐Wei
Venue
Small
Publisher
Wiley
Published
2012-07-02
Volume
8
Issue
18
Pages
2869-2877
ISSN
  • 1613-6810 (ISSN-L)
  • 1613-6829
OpenAlex
W2118948952
Cited by
149
References
35
Citations by year
2026: 3 · 2025: 2 · 2024: 5 · 2023: 6 · 2022: 11 · 2021: 14 · 2020: 16 · 2019: 16 · 2018: 14 · 2017: 15 · 2016: 12 · 2015: 6 · 2014: 13 · 2013: 13 · 2012: 3
FWCI
4.61
Type
journal article
Language
en
Open access
closed
Locations
  • Small (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Science Council
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
  • Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
neural stem cells, electrical stimulation, neuronal differentiation, neurite outgrowth, neuronal maturation, substrate topography, nerve regeneration, chemical vapor deposition
MeSH terms
Animals, Cell Differentiation, Electric Stimulation, Microscopy, Electron, Scanning, Neurites, Nanotubes, Carbon, Rats, Neural Stem Cells

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review