The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
Optic Vesicle-like Structures Derived from Human Pluripotent Stem Cells Facilitate a Customized Approach to Retinal Disease Treatment
Open accessAbstract
Differentiation methods for human induced pluripotent stem cells (hiPSCs) typically yield progeny from multiple tissue lineages, limiting their use for drug testing and autologous cell transplantation. In particular, early retina and forebrain derivatives often intermingle in pluripotent stem cell cultures, owing to their shared ancestry and tightly coupled development. Here, we demonstrate that three-dimensional populations of retinal progenitor cells (RPCs) can be isolated from early forebrain populations in both human embryonic stem cell and hiPSC cultures, providing a valuable tool for developmental, functional, and translational studies. Using our established protocol, we identified a transient population of optic vesicle (OV)-like structures that arose during a time period appropriate for normal human retinogenesis. These structures were independently cultured and analyzed to confirm their multipotent RPC status and capacity to produce physiologically responsive retinal cell types, including photoreceptors and retinal pigment epithelium (RPE). We then applied this method to hiPSCs derived from a patient with gyrate atrophy, a retinal degenerative disease affecting the RPE. RPE generated from these hiPSCs exhibited a disease-specific functional defect that could be corrected either by pharmacological means or following targeted gene repair. The production of OV-like populations from human pluripotent stem cells should facilitate the study of human retinal development and disease and advance the use of hiPSCs in personalized medicine.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The Metabolic and Molecular Bases of Inherited Disease
Scriver, R. C. (1995) · Medical Entomology and Zoology · Cited by 12,101
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007) · Science · doi:10.1126/science.1151526 · Cited by 10,310
Self-citationHuman induced pluripotent stem cells free of vector and transgene sequences
Yu, J., Hu, K., Smuga-Otto, K., Tian, S., Stewart, R., Slukvin, I. I., & Thomson, J. A. (2009) · Science · doi:10.1126/science.1172482 · Cited by 2,386
Self-citationDisease-Specific Induced Pluripotent Stem Cells.
Park, I.-H., Arora, N., Huo, H., Maherali, N., Ahfeldt, T., Shimamura, A., Lensch, M. W., Cowan, C., Hochedlinger, K., & Daley, G. Q. (2008) · Cell · doi:10.1016/j.cell.2008.07.041 · Cited by 2,196
Self-organizing optic-cup morphogenesis in three-dimensional culture
Eiraku, M., Takata, N., Ishibashi, H., Kawada, M., Sakakura, E., Okuda, S., Sekiguchi, K., Adachi, T., & Sasai, Y. (2011) · Nature · doi:10.1038/nature09941 · Cited by 2,001
Induced pluripotent stem cells from a spinal muscular atrophy patient
Ebert, A. D., Yu, J., Rose, F. F., Mattis, V. B., Lorson, C. L., Thomson, J. A., & Svendsen, C. N. (2009) · Nature · doi:10.1038/nature07677 · Cited by 1,441
Self-citationEmbryonic stem cell differentiation: emergence of a new era in biology and medicine
Keller, G. (2005) · Genes & Development · doi:10.1101/gad.1303605 · Cited by 1,151
Elementary and global aspects of calcium signalling.
Berridge, M. J. (1997) · The Journal of Physiology · doi:10.1113/jphysiol.1997.sp021927 · Cited by 1,018
Hu, B.-Y., Weick, J. P., Yu, J., Ma, L.-X., Zhang, X.-Q., Thomson, J. A., & Zhang, S.-C. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0910012107 · Cited by 1,013
Self-citationInduced pluripotency: history, mechanisms, and applications
Stadtfeld, M., & Hochedlinger, K. (2010) · Genes & Development · doi:10.1101/gad.1963910 · Cited by 829
Pluripotency and Cellular Reprogramming: Facts, Hypotheses, Unresolved Issues
Hanna, J. H., Saha, K., & Jaenisch, R. (2010) · Cell · doi:10.1016/j.cell.2010.10.008 · Cited by 698
Efficient generation of retinal progenitor cells from human embryonic stem cells
Lamba, D. A., Karl, M. O., Ware, C. B., & Reh, T. A. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0601990103 · Cited by 689
Early Eye Development in Vertebrates
Chow, R. L., & Lang, R. A. (2001) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.17.1.255 · Cited by 642
Osakada, F., Ikeda, H., Mandai, M., Wataya, T., Watanabe, K., Yoshimura, N., Akaike, A., Sasai, Y., & Takahashi, M. (2008) · Nature Biotechnology · doi:10.1038/nbt1384 · Cited by 633
Modeling early retinal development with human embryonic and induced pluripotent stem cells
Meyer, J. S., Shearer, R. L., Capowski, E. E., Wright, L. S., Wallace, K. A., McMillan, E. L., Zhang, S.-C., & Gamm, D. M. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0905245106 · Cited by 601
Self-citationCyclic GMP-Activated Conductance of Retinal Photoreceptor Cells
Yau, K. W., & Baylor, D. A. (1989) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.12.030189.001445 · Cited by 595
Phototransduction Motifs and Variations
Yau, K.-W., & Hardie, R. C. (2009) · Cell · doi:10.1016/j.cell.2009.09.029 · Cited by 492
Idelson, M., Alper, R., Obolensky, A., Ben-Shushan, E., Hemo, I., Yachimovich-Cohen, N., Khaner, H., Smith, Y., Wiser, O., Gropp, M., et al. (2009) · Cell stem cell · doi:10.1016/j.stem.2009.07.002 · Cited by 486
Klimanskaya, I., Hipp, J., Rezai, K. A., West, M., Atala, A., & Lanza, R. (2004) · Cloning and Stem Cells · doi:10.1089/clo.2004.6.217 · Cited by 437
Derivation of functional retinal pigmented epithelium from induced pluripotent stem cells
Buchholz, D. E., Hikita, S. T., Rowland, T. J., Friedrich, A. M., Hinman, C. R., Johnson, L. V., & Clegg, D. O. (2009) · Stem Cells · doi:10.1002/stem.189 · Cited by 417
Lamba, D. A., McUsic, A., Hirata, R. K., Wang, P.-R., Russell, D., & Reh, T. A. (2010) · PLoS ONE · doi:10.1371/journal.pone.0008763 · Cited by 394
Generating neuronal diversity in the retina: one for nearly all
Marquardt, T., & Gruss, P. (2002) · Trends in Neurosciences · doi:10.1016/s0166-2236(00)02028-2 · Cited by 361
Phototransduction mechanism in retinal rods and cones. The Friedenwald Lecture.
Yau, K. (1994) · PubMed · Cited by 331
Nguyen, M.-T. T., & Arnheiter, H. (2000) · Development · doi:10.1242/dev.127.16.3581 · Cited by 287
Fuhrmann, S., Levine, E. M., & Reh, T. A. (2000) · Development · doi:10.1242/dev.127.21.4599 · Cited by 278
Retinal cell fate determination and bHLH factors
Hatakeyama, J., & Kageyama, R. (2004) · Seminars in Cell & Developmental Biology · doi:10.1016/j.semcdb.2003.09.005 · Cited by 269
Human embryonic stem cells: prospects for development
Pera, M. F., & Trounson, A. O. (2004) · Development · doi:10.1242/dev.01451 · Cited by 237
Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors
Ahmad, I., Leinders-Zufall, T., Kocsis, J. D., Shepherd, G. M., Zufall, F., & Barnstable, C. J. (1994) · Neuron · doi:10.1016/0896-6273(94)90160-0 · Cited by 235
Chx10 repression of Mitf is required for the maintenance of mammalian neuroretinal identity
Horsford, D. J., Nguyen, M.-T. T., Sellar, G. C., Kothary, R., Arnheiter, H., & McInnes, R. R. (2005) · Development · doi:10.1242/dev.01571 · Cited by 217
Modeling Retinal Degeneration Using Patient-Specific Induced Pluripotent Stem Cells
Jin, Z.-B., Okamoto, S., Osakada, F., Homma, K., Assawachananont, J., Hirami, Y., Iwata, T., & Takahashi, M. (2011) · PLoS ONE · doi:10.1371/journal.pone.0017084 · Cited by 216
Banin, E., Obolensky, A., Idelson, M., Hemo, I., Reinhardtz, E., Pikarsky, E., Ben-Hur, T., & Reubinoff, B. (2006) · Stem Cells · doi:10.1634/stemcells.2005-0009 · Cited by 214
Molecular signature of primary retinal pigment epithelium and stem-cell-derived RPE cells
Liao, J.-L., Yu, J., Huang, K., Hu, J., Diemer, T., Ma, Z., Dvash, T., Yang, X.-J., Travis, G. H., Williams, D. S., et al. (2010) · Human Molecular Genetics · doi:10.1093/hmg/ddq341 · Cited by 190
Rowan, S., Chen, C.-M. A., Young, T. L., Fisher, D. E., & Cepko, C. L. (2004) · Development · doi:10.1242/dev.01300 · Cited by 162
Bharti, K., Liu, W., Csermely, T., Bertuzzi, S., & Arnheiter, H. (2008) · Development · doi:10.1242/dev.014142 · Cited by 160
Genetic correction and analysis of induced pluripotent stem cells from a patient with gyrate atrophy
Howden, S. E., Gore, A., Li, Z., Fung, H.-L., Nisler, B. S., Nie, J., Chen, G., McIntosh, B. E., Gulbranson, D. R., Diol, N. R., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1103388108 · Cited by 154
Self-citationFujimura, N., Taketo, M. M., Mori, M., Kořínek, V., & Kozmík, Z. (2009) · Developmental Biology · doi:10.1016/j.ydbio.2009.07.002 · Cited by 149
Embryology of the eye and its adnexae.
Barishak, Y. R. (1992) · PubMed · Cited by 145
Peterson, W. M., Meggyesy, C., Yu, K., & Miller, S. S. (1997) · Journal of Neuroscience · doi:10.1523/jneurosci.17-07-02324.1997 · Cited by 130
Current views on eye development
Oliver, G. C., & Gruß, P. (1997) · Trends in Neurosciences · doi:10.1016/s0166-2236(97)01082-5 · Cited by 109
Induced pluripotent stem cells and reprogramming: seeing the science through the hype
Belmonte, J. C. I., Ellis, J., Hochedlinger, K., & Yamanaka, S. (2009) · Nature Reviews Genetics · doi:10.1038/nrg2700 · Cited by 98
Wang, T., Steel, G., Milam, A. H., & Valle, D. L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.3.1224 · Cited by 91
Wang, T., Milam, A. H., Steel, G., & Valle, D. L. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118730 · Cited by 80
Picaud, S. A., Pattnaik, B. R., Hicks, D., et al. (1998) · The Journal of Physiology · doi:10.1111/j.1469-7793.1998.033by.x · Cited by 75
Self-citationA Novel Serum-Free Method for Culturing Human Prenatal Retinal Pigment Epithelial Cells
Gamm, D. M., Melvan, J. N., Shearer, R. L., Pinilla, I., Sabat, G., Svendsen, C. N., & Wright, L. S. (2008) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.07-0777 · Cited by 73
Self-citationMicroarray Characterization of Human Embryonic Stem Cell–Derived Retinal Cultures
Lamba, D. A., & Reh, T. A. (2011) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.10-6504 · Cited by 50
The developing and evolving retina: Using time to organize form
Finlay, B. L. (2007) · Brain Research · doi:10.1016/j.brainres.2007.07.005 · Cited by 48
Cyclic nucleotide-gated channels in visual and olfactory transduction
Menini, A. (1995) · Biophysical Chemistry · doi:10.1016/0301-4622(94)00153-b · Cited by 42
Molecular Aspects of Vertebrate Retinal Development
Zhang, S. S.-M., Fu, X.-Y., & Barnstable, C. J. (2002) · Molecular Neurobiology · doi:10.1385/mn:26:2-3:137 · Cited by 40
Balse, E., Tessier, L., Fuchs, C., Forster, V., Sahel, J., & Picaud, S. A. (2004) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.04-0695 · Cited by 29
Glycine receptors in a population of adult mammalian cones
Balse, E., Tessier, L., Forster, V., Roux, M. J., Sahel, J., & Picaud, S. A. (2006) · The Journal of Physiology · doi:10.1113/jphysiol.2005.100891 · Cited by 23
Directed Differentiation of Human Induced Pluripotent Stem Cells: A Retina Perspective
Gamm, D. M., & Meyer, J. S. (2010) · Regenerative Medicine · doi:10.2217/rme.10.28 · Cited by 22
Self-citation
Cited by
Self-formation of optic cups and storable stratified neural retina from human ESCs
Nakano, T., Ando, S., Takata, N., Kawada, M., Muguruma, K., Sekiguchi, K., Saito, K., Yonemura, S., Eiraku, M., & Sasai, Y. (2012) · Cell stem cell · doi:10.1016/j.stem.2012.05.009 · Cited by 1,447
Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs
Zhong, X., Gutierrez, C., Xue, T., Hampton, C., Vergara, M. N., Cao, L.-H., Peters, A., Park, T. S., Zambidis, E. T., Meyer, J. S., et al. (2014) · Nature Communications · doi:10.1038/ncomms5047 · Cited by 970
Self-citationIn vitro generation of human pluripotent stem cell derived lung organoids
Dye, B. R., Hill, D. R., Ferguson, M. A., Tsai, Y.-H., Nagy, M. S., Dyal, R., Wells, J. M., Mayhew, C. N., Nattiv, R., Klein, O. D., et al. (2015) · eLife · doi:10.7554/elife.05098 · Cited by 819
Cell Types of the Human Retina and Its Organoids at Single-Cell Resolution
Cowan, C. S., Renner, M., De Gennaro, M., Gross-Scherf, B., Goldblum, D., Hou, Y., Munz, M., Rodrigues, T. M., Krol, J., Szikra, T., et al. (2020) · Cell · doi:10.1016/j.cell.2020.08.013 · Cited by 654
Cuenca, N., Fernández-Sánchez, L., Campello, L., Maneu, V., De la Villa, P., Lax, P., & Pinilla, I. (2014) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2014.07.001 · Cited by 401
Self-citation
Metadata
- Authors
- Meyer, J. S., Howden, S. E., Wallace, K. A., Verhoeven, A. D., Wright, L. S., Capowski, E. E., Pinilla, I., Martin, J. M., Tian, S., Stewart, R., Pattnaik, B., Thomson, J. A., & Gamm, D. M.
- Venue
- Stem Cells
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-07-26
- Volume
- 29
- Issue
- 8
- Pages
- 1206-1218
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W1994009311
- Cited by
- 469
- References
- 51
- FWCI
- 12.11
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
