The wide-Ranging phenotypes of ergosterol biosynthesis mutants, and implications for microbial cell factories
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Yeast strains have been used extensively as robust microbial cell factories for the production of bulk and fine chemicals, including biofuels (bioethanol), complex pharmaceuticals (antimalarial drug artemisinin and opioid pain killers), flavours, and fragrances (vanillin, nootkatone, and resveratrol). In many cases, it is of benefit to suppress or modify ergosterol biosynthesis during strain engineering, for example, to increase thermotolerance or to increase metabolic flux through an alternate pathway. However, the impact of modifying ergosterol biosynthesis on engineered strains is discussed sparsely in literature, and little attention has been paid to the implications of these modifications on the general health and well-being of yeast. Importantly, yeast with modified sterol content exhibit a wide range of phenotypes, including altered organization and dynamics of plasma membrane, altered susceptibility to chemical treatment, increased tolerance to high temperatures, and reduced tolerance to other stresses such as high ethanol, salt, and solute concentrations. Here, we review the wide-ranging phenotypes of viable Saccharomyces cerevisiae strains with altered sterol content and discuss the implications of these for yeast as microbial cell factories.
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The single deletion of the majority of ERG genes within the late pathway is lethal under standard growth conditions without the addition of ergosterol.
“The single deletion of the majority of ergosterol biosynthetic (ERG) genes within the late pathway is lethal under standard growth conditions without the addition of ergosterol.”
From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [16]
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Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae
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- Authors
- Johnston, E. J., Moses, T., & Rosser, S. J.
- Affiliations
- University of Edinburgh (GB)
- Corresponding author
- Johnston, Emily J.
- Venue
- Yeast
- Publisher
- Wiley
- Published
- 2020-01
- Volume
- 37
- Issue
- 1
- Pages
- 27-44
- ISSN
- 0749-503X (ISSN-L)
- 1097-0061
- OpenAlex
- W2992904553
- Cited by
- 74
- References
- 135
- Citations by year
- 2026: 7 · 2025: 13 · 2024: 15 · 2023: 15 · 2022: 11 · 2021: 8 · 2020: 5
- FWCI
- 0.53
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Yeast (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, cc-by)
- Funders
- Biotechnology and Biological Sciences Research Council (IBCatalyst Project 102297, Project BB/S017712/1)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Keywords
- ergosterol biosynthesis, Saccharomyces cerevisiae, yeast phenotypes, plasma membrane dynamics, heat tolerance, stress tolerance, ethanol tolerance, salt tolerance, sterol content, microbial cell factories, strain engineering, artemisinin production, bioethanol production
- MeSH terms
- Ergosterol, Fermentation, Mutation, Phenotype, Saccharomyces cerevisiae, Sterols, Biofuels, Metabolic Engineering
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