Cited by 255Nitric Oxide and Endothelin EffectsAntioxidant Activity and Oxidative StressRedox biology and oxidative stress

The L-Arginine: Nitric Oxide Pathway Is the Major Source of Plasma Nitrite in Fasted Humans

Rhodes, P.M., Leone, A.M., Francis, P.L., Struthers, A.D., Moncada, S.·1995·Biochemical and Biophysical Research Communications

Abstract

15N guanidino-labelled L-arginine was infused into fasted human volunteers giving, at equilibrium, a stable 1:10 ratio of 15N to 14N arginine in the plasma. Separate GC-MS assays were used to compare the degree of enrichment of plasma arginine, nitrite and nitrate and thus define the quantitative relationship between the L-arginine:nitric oxide (NO) pathway and the formation of these oxides of nitrogen. 15N nitrite enrichments rose to 8.3% (SD 0.5), five hours after the start of the infusion. In contrast, 15N nitrate enrichments apparently rose to only 1.6% (SD 0.4) at this time. This discrepancy could be explained by our finding that the commonly used Tesch GC-MS nitrate assay is subject to considerable interference from non-nitrate sources in plasma. Taking this into account, nitrate enrichments were similar to those observed for plasma nitrite. These results therefore indicate that the measurement of these compounds in plasma is a valid indicator of NO generation in fasted humans.

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Crossref · 10.1006/bbrc.1995.1541

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Authors
Rhodes, P. M., Leone, A. M., Francis, P. L., Struthers, A. D., & Moncada, S.
Affiliations
Ninewells Hospital (GB)
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
1995-04
Volume
209
Issue
2
Pages
590-596
ISSN
0006-291X (ISSN-L)
OpenAlex
W2049563995
Cited by
254
References
0
Citations by year
2025: 2 · 2024: 3 · 2022: 4 · 2021: 3 · 2020: 5 · 2019: 5 · 2018: 8 · 2017: 6 · 2016: 3 · 2015: 9 · 2014: 12 · 2013: 9 · 2012: 9
FWCI
3.65
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
  • Discovery Research Portal (University of Dundee) (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
L-arginine, nitric oxide, nitrite, nitrate, arginine infusion, fasting state, plasma nitrite, nitrogen oxides
MeSH terms
Adult, Arginine, Fasting, Humans, Male, Gas Chromatography-Mass Spectrometry, Nitric Oxide, Nitrites

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