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Cited by 163Vitamin C and Antioxidants ResearchAmino Acid Enzymes and MetabolismDrug Transport and Resistance Mechanisms

The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.

Human Placental Sodium-Dependent Vitamin C Transporter (SVCT2): Molecular Cloning and Transport Function

Rajan, D.Prasanna, Huang, Wei, Dutta, Binita, Devoe, Lawrence D., Leibach, Frederich H., Ganapathy, Vadivel, Prasad, Puttur D.·1999·Biochemical and Biophysical Research Communications
DOI 10.1006/bbrc.1999.1272
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Abstract

We report here on the cloning and functional characterization of human SVCT2, a sodium-dependent vitamin C (ascorbate) transporter. The hSVCT2 cDNA obtained from a human placental choriocarcinoma cell cDNA library, codes for a protein of 650 amino acids with a predicted molecular mass of 70 kDa. At the level of amino acid sequence, the human SVCT2 exhibits 95% identity to its rat homolog. When functionally expressed in mammalian cells, hSVCT2 induces the transport of ascorbic acid. The transport process induced by hSVCT2 is Na(+)-dependent and is specific for ascorbate. The Michaelis-Menton constant (K(t)) for the transport of ascorbate in cDNA-transfected cells is 69 +/- 5 microM. The relationship between the cDNA-specific uptake rate of ascorbate and Na(+) concentration is sigmoidal with a Na(+):ascorbate stoichiometry of 2:1. Northern blot analysis shows that SVCT2-specific transcripts are present in heart, brain, placenta, and liver and is absent in lung and skeletal muscle. The size of the principal transcript is approximately 7.5 kb.

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  1. Huang, 1997, Invest. Ophthalmol. Visual Sci., 38, 1578
  2. Altschul, 1990, J. Mol. Biol., 215, 403, 10.1016/S0022-2836(05)80360-2 · DOI · Paper page

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Metadata

Authors
Rajan, D. P., Huang, W., Dutta, B., Devoe, L. D., Leibach, F. H., Ganapathy, V., & Prasad, P. D.
Affiliations
Augusta University (US)
Corresponding author
Prasad, Puttur D.
ORCID
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
1999-09
Volume
262
Issue
3
Pages
762-768
ISSN
0006-291X (ISSN-L)
DOI
10.1006/bbrc.1999.1272
OpenAlex
W2005413052
Cited by
162
References
15
Citations by year
2025: 2 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 7 · 2020: 5 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 6 · 2015: 1 · 2014: 5 · 2013: 11 · 2012: 9
FWCI
6.50
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD 33347, R01HD033347)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Keywords
SVCT2, sodium-dependent vitamin C transporters, ascorbate transport, vitamin C, placenta, gene cloning, sodium dependence, Michaelis-Menten kinetics, tissue distribution, cDNA expression, choriocarcinoma cells
MeSH terms
Amino Acid Sequence, Animals, Ascorbic Acid, Base Sequence, Biological Transport, Cell Line, Choriocarcinoma, Cloning, Molecular, Female, Humans, Kinetics, Molecular Sequence Data, Pregnancy, Proteins, Recombinant Proteins, Sodium, Substrate Specificity, Transfection, Tumor Cells, Cultured, Uterine Neoplasms, Gene Library, Symporters, Organic Anion Transporters, Sodium-Dependent, Rats, Sodium-Coupled Vitamin C Transporters

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