Cited by 163Vitamin C and Antioxidants ResearchAmino Acid Enzymes and MetabolismDrug Transport and Resistance Mechanisms

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Human Placental Sodium-Dependent Vitamin C Transporter (SVCT2): Molecular Cloning and Transport Function

Rajan, D.Prasanna, Huang, Wei, Dutta, Binita, Devoe, Lawrence D., Leibach, Frederich H., Ganapathy, Vadivel, Prasad, Puttur D.·1999·Biochemical and Biophysical Research Communications

DOI 10.1006/bbrc.1999.1272

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Abstract

We report here on the cloning and functional characterization of human SVCT2, a sodium-dependent vitamin C (ascorbate) transporter. The hSVCT2 cDNA obtained from a human placental choriocarcinoma cell cDNA library, codes for a protein of 650 amino acids with a predicted molecular mass of 70 kDa. At the level of amino acid sequence, the human SVCT2 exhibits 95% identity to its rat homolog. When functionally expressed in mammalian cells, hSVCT2 induces the transport of ascorbic acid. The transport process induced by hSVCT2 is Na(+)-dependent and is specific for ascorbate. The Michaelis-Menton constant (K(t)) for the transport of ascorbate in cDNA-transfected cells is 69 +/- 5 microM. The relationship between the cDNA-specific uptake rate of ascorbate and Na(+) concentration is sigmoidal with a Na(+):ascorbate stoichiometry of 2:1. Northern blot analysis shows that SVCT2-specific transcripts are present in heart, brain, placenta, and liver and is absent in lung and skeletal muscle. The size of the principal transcript is approximately 7.5 kb.

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Authors: Rajan, D. P., Huang, W., Dutta, B., Devoe, L. D., Leibach, F. H., Ganapathy, V., & Prasad, P. D.
Affiliations: Augusta University (US)
Corresponding author: Prasad, Puttur D.
ORCID: Huang, Wei
Devoe, Lawrence D.
Ganapathy, Vadivel
Prasad, Puttur D.
Venue: Biochemical and Biophysical Research Communications
Publisher: Elsevier BV
Published: 1999-09
Volume: 262
Issue: 3
Pages: 762-768
ISSN: 0006-291X (ISSN-L)
DOI: 10.1006/bbrc.1999.1272
OpenAlex: W2005413052
Cited by: 162
References: 15
Citations by year: 2025: 2 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 7 · 2020: 5 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 6 · 2015: 1 · 2014: 5 · 2013: 11 · 2012: 9
FWCI: 6.50
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Biochemical and Biophysical Research Communications (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health
Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD 33347, R01HD033347)
Topics: Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Keywords: SVCT2, sodium-dependent vitamin C transporters, ascorbate transport, vitamin C, placenta, gene cloning, sodium dependence, Michaelis-Menten kinetics, tissue distribution, cDNA expression, choriocarcinoma cells
MeSH terms: Amino Acid Sequence, Animals, Ascorbic Acid, Base Sequence, Biological Transport, Cell Line, Choriocarcinoma, Cloning, Molecular, Female, Humans, Kinetics, Molecular Sequence Data, Pregnancy, Proteins, Recombinant Proteins, Sodium, Substrate Specificity, Transfection, Tumor Cells, Cultured, Uterine Neoplasms, Gene Library, Symporters, Organic Anion Transporters, Sodium-Dependent, Rats, Sodium-Coupled Vitamin C Transporters

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Human Placental Sodium-Dependent Vitamin C Transporter (SVCT2): Molecular Cloning and Transport Function

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