The DOI matches its Crossref record; 48 of the 49 references it cites were found in a registry or an index.
Artifacts in Cell Culture: Rapid Generation of Hydrogen Peroxide on Addition of (−)-Epigallocatechin, (−)-Epigallocatechin Gallate, (+)-Catechin, and Quercetin to Commonly Used Cell Culture Media
Abstract
There is considerable current interest in the possible beneficial health effects of quercetin, catechins, epigallocatechins, epigallocatechin gallates, and related phenolic compounds found in teas, wines, and other plant products. As a result, many laboratories are studying the effects of these compounds on cells in culture. The present paper shows that addition of these compounds to commonly used cell culture media leads to generation of substantial amounts of hydrogen peroxide (H(2)O(2)). Dulbecco's modified Eagle medium gives the highest H(2)O(2) level for all the compounds tested, with levels reaching >400 microM within 2 h for addition of 1 mM concentrations of gallic acid, epigallocatechin gallate, and epigallocatechin. Catechin and quercetin produced lower, but still significant, levels of H(2)O(2). McCoy's 5A and RPMI 1640 media also promoted H(2)O(2) production from the above phenolic compounds. This rapid generation of H(2)O(2) could account for some or all of the reported effects of phenolic compounds on cells in culture.
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“One interpretation for these disappointing results, that is consistent with the general theme of this review, involves the recognition that a variety of antioxidants can in fact act as pro-oxidants and thus actually promote the production of OH• radicals in the presence of inappropriately or inadequately liganded Fe(II) [130,528,1602,1604,1659,1660].”
From Iron behaving badly: inappropriate iron chelation as a major contributor to the aetiology of vascular and other progressive inflammatory and degenerative diseases (2009) · cites this paper as [1659]
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1 of the 49 references couldn’t be matched to a known paper.
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Halliwell, B., & Whiteman, M. (2004) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0705776 · Cited by 2,238
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Self-citation(–)-Epicatechin mediates beneficial effects of flavanol-rich cocoa on vascular function in humans
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Metadata
- Authors
- Long, L. H., Clement, M. V., & Halliwell, B.
- Affiliations
- National University of Singapore (SG)
- Corresponding author
- Halliwell, Barry
- ORCID
- Halliwell, Barry
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2000-06
- Volume
- 273
- Issue
- 1
- Pages
- 50-53
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2018264979
- Cited by
- 390
- References
- 49
- Citations by year
- 2026: 6 · 2025: 1 · 2024: 4 · 2023: 10 · 2022: 8 · 2021: 9 · 2020: 4 · 2019: 7 · 2018: 5 · 2017: 9 · 2016: 23 · 2015: 10 · 2014: 12 · 2013: 16 · 2012: 23
- FWCI
- 10.96
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Food Science › Tea Polyphenols and Effects
- Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
- Keywords
- hydrogen peroxide production, cell culture media, epigallocatechin, epigallocatechin gallate, catechins, quercetin, phenolic compounds, gallic acid, oxidative stress, DMEM, RPMI 1640, tea polyphenols
- MeSH terms
- Catechin, Culture Media, Dose-Response Relationship, Drug, Flavonoids, Hydrogen Peroxide, Quercetin, Time Factors, Artifacts, Cell Culture Techniques
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