Cited by 390Phytochemicals and Antioxidant ActivitiesTea Polyphenols and EffectsFree Radicals and Antioxidants

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Artifacts in Cell Culture: Rapid Generation of Hydrogen Peroxide on Addition of (−)-Epigallocatechin, (−)-Epigallocatechin Gallate, (+)-Catechin, and Quercetin to Commonly Used Cell Culture Media

Long, Lee Hua, Clement, Marie Veronique, Halliwell, Barry·2000·Biochemical and Biophysical Research Communications

DOI 10.1006/bbrc.2000.2895

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Abstract

There is considerable current interest in the possible beneficial health effects of quercetin, catechins, epigallocatechins, epigallocatechin gallates, and related phenolic compounds found in teas, wines, and other plant products. As a result, many laboratories are studying the effects of these compounds on cells in culture. The present paper shows that addition of these compounds to commonly used cell culture media leads to generation of substantial amounts of hydrogen peroxide (H(2)O(2)). Dulbecco's modified Eagle medium gives the highest H(2)O(2) level for all the compounds tested, with levels reaching >400 microM within 2 h for addition of 1 mM concentrations of gallic acid, epigallocatechin gallate, and epigallocatechin. Catechin and quercetin produced lower, but still significant, levels of H(2)O(2). McCoy's 5A and RPMI 1640 media also promoted H(2)O(2) production from the above phenolic compounds. This rapid generation of H(2)O(2) could account for some or all of the reported effects of phenolic compounds on cells in culture.

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Authors: Long, L. H., Clement, M. V., & Halliwell, B.
Affiliations: National University of Singapore (SG)
Corresponding author: Halliwell, Barry
ORCID: Halliwell, Barry
Venue: Biochemical and Biophysical Research Communications
Publisher: Elsevier BV
Published: 2000-06
Volume: 273
Issue: 1
Pages: 50-53
ISSN: 0006-291X (ISSN-L)
DOI: 10.1006/bbrc.2000.2895
OpenAlex: W2018264979
Cited by: 390
References: 49
Citations by year: 2026: 6 · 2025: 1 · 2024: 4 · 2023: 10 · 2022: 8 · 2021: 9 · 2020: 4 · 2019: 7 · 2018: 5 · 2017: 9 · 2016: 23 · 2015: 10 · 2014: 12 · 2013: 16 · 2012: 23
FWCI: 10.96
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Biochemical and Biophysical Research Communications (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
Life Sciences › Agricultural and Biological Sciences › Food Science › Tea Polyphenols and Effects
Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
Keywords: hydrogen peroxide production, cell culture media, epigallocatechin, epigallocatechin gallate, catechins, quercetin, phenolic compounds, gallic acid, oxidative stress, DMEM, RPMI 1640, tea polyphenols
MeSH terms: Catechin, Culture Media, Dose-Response Relationship, Drug, Flavonoids, Hydrogen Peroxide, Quercetin, Time Factors, Artifacts, Cell Culture Techniques

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Artifacts in Cell Culture: Rapid Generation of Hydrogen Peroxide on Addition of (−)-Epigallocatechin, (−)-Epigallocatechin Gallate, (+)-Catechin, and Quercetin to Commonly Used Cell Culture Media

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