Disruption of klotho Gene Causes an Abnormal Energy Homeostasis in Mice
Abstract
klotho mice, which genetically lack klotho gene expression, are characterized with various systemic phenotypes resembling human aging, and also with growth retardation. Here we show that klotho mice have a barely detectable amount of the white adipose tissue but their brown adipose tissue (BAT) is comparably preserved. Glucose tolerance and insulin sensitivity in klotho mice are increased compared to those in wild-type mice as revealed by intraperitoneal glucose and insulin tolerance tests. Uncoupling protein-1 gene expression of BAT and body temperature in klotho mice are lower than those in wild-type mice, suggesting that klotho mice have less energy expenditure than wild-type mice. Histological examination suggests that klotho mice possess less energy storage than wild-type mice with respect to glycogen in the liver and lipid in BAT. All these changes of parameters for energy homeostasis in klotho mice are very similar to those reported under food-restricted conditions. However, the amount of food intake is not different between klotho and wild-type mice when normalized for body weight. The present study elucidates the importance of klotho gene expression for the maintenance of normal energy homeostasis.
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Disruption of the klotho gene in mice results in almost complete absence of WAT, while BAT is present but reduced in size and UCP1 protein expression; these mice also have lower core body temperature.
“Disruption of the klotho gene in mice results in almost complete absence of WAT, while BAT is present but reduced in size and UCP1 protein expression. These mice also have lower core body temperature 108.”
From Cellular bioenergetics as a target for obesity therapy (2010) · cites this paper as [108]
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- Authors
- Mori, K., Yahata, K., Mukoyama, M., Suganami, T., Makino, H., Nagae, T., Masuzaki, H., Ogawa, Y., Sugawara, A., Nabeshima, Y.-I., & Nakao, K.
- Affiliations
- Kyoto University (JP)
- Corresponding author
- Mori, Kiyoshi
- ORCID
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2000-11
- Volume
- 278
- Issue
- 3
- Pages
- 665-670
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2147279583
- Cited by
- 65
- References
- 20
- Citations by year
- 2026: 1 · 2025: 2 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 3 · 2019: 3 · 2016: 4 · 2015: 3 · 2013: 3 · 2012: 3
- FWCI
- 2.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Smoking Research Foundation
- Salt Science Research Foundation
- Japan Foundation for Aging and Health
- Japan Society for the Promotion of Science
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Nephrology › Parathyroid Disorders and Treatments
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Keywords
- Klotho gene, energy homeostasis, brown adipose tissue, white adipose tissue, UCP1, glucose tolerance, insulin sensitivity, energy expenditure, glycogen storage, fat deposition, aging, dietary restriction
- MeSH terms
- Klotho Proteins, Adipose Tissue, Aging, Animals, Blood Glucose, Body Weight, Adipose Tissue, Brown, Energy Metabolism, Glucuronidase, Homeostasis, Humans, Insulin, Liver, Liver Glycogen, Male, Membrane Proteins, Mice, Mutant Strains, Organ Size, Pancreas, Phosphoenolpyruvate Carboxykinase (GTP), Models, Animal, Mice
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