Purification and Characterization of an enzyme involved in biochemical transformation of arteannuin B to artemisinin from Artemisia annua
Abstract
The protein involved in the conversion of arteannuin B to artemisinin has been purified from the leaves of Artemisia annua. The pure protein found to be homogenous on Native gel electrophoresis showed two major bands of 21 and 11 kDa on 12% SDS-PAGE. Molecular weight estimation of native protein indicated an apparent molecular mass of 66,000 Daltons. This protein is able to achieve 58% conversion. It has a K(m) of 0.5 mM for arteannuin B and a pH optima between 7.0-7.2. It is maximally active at 30 degrees C.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A microbial system capable of converting arteannuin B (462) to artemisinin (495) has been described, and an enzyme with the appropriate activity has been purified.
“A microbial system which is capable of converting arteannuin B (462) to artemisinin (495) has recently been described [309] and an enzyme with the appropriate activity has also been purified [310,311].”
From The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao) (2010) · cites this paper as [309]
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 315,771 · see citing works
Protein Purification: Principles and Practice
Scopes, R. K. (1982) · Cited by 1,029 · see citing works
Protein purification: Principles and practice
Jakoby, W. B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90759-5 · Cited by 420 · see citing works
Current status of artemisinin and its derivatives as antimalarial drugs
Dhingra, V., Rao, K. V., & Narasu, M. L. (1999) · Life Sciences · doi:10.1016/s0024-3205(99)00356-2 · Cited by 241 · see citing works
Self-citationSELECTION AND BREEDING FOR HIGH ARTEMISININ (QINGHAOSU) YIELDING STRAINS OF ARTEMISIA ANNUA
Delabays, N., Collet, G. F., & Benakis, A. (1993) · Acta Horticulturae · doi:10.17660/actahortic.1993.330.24 · Cited by 72 · see citing works
Bioconversion of arteannuin B to artemisinin
Nair, M. S. R., & Basile, D. V. (1993) · Journal of Natural Products · doi:10.1021/np50099a015 · Cited by 62 · see citing works
Scopes, R. K. (1982) · Springer eBooks · doi:10.1007/978-1-4757-1770-9_3 · Cited by 46 · see citing works
Antimicrobial activity of artemisinin and its precursors.
Dhingra, V., Pakki, S., & Narasu, M. L. (2000) · Cited by 32 · see citing works
Self-citationArtemisinin: present status and perspectives
Dhingra, V., Rao, K. V., & Narasu, M. L. (1999) · Biochemical Education · doi:10.1016/s0307-4412(98)00282-9 · Cited by 21 · see citing works
Self-citationPartial purification of proteins involved in the bioconversion of arteannuin B to artemisinin
Dhingra, V (2000) · Bioresource Technology · doi:10.1016/s0960-8524(99)00152-2 · Cited by 17 · see citing works
Self-citation
Flagged first, then by citations
1 of the 11 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Production of isoprenoid pharmaceuticals by engineered microbes
Chang, M. C. Y., & Keasling, J. D. (2006) · Nature Chemical Biology · doi:10.1038/nchembio836 · Cited by 388 · see citing works
The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao)
Brown, G. D. (2010) · Molecules · doi:10.3390/molecules15117603 · Cited by 363 · see citing works
Artemisinin: current state and perspectives for biotechnological production of an antimalarial drug
Liu, C., Zhao, Y., & Wang, Y. (2006) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-006-0452-0 · Cited by 158 · see citing works
Shasany, A. K., Shukla, A. K., & Khanuja, S. P. S. (2007) · Springer eBooks · doi:10.1007/978-3-540-34538-1_9 · Cited by 147 · see citing works
Relative expression of genes of terpene metabolism in different tissues of Artemisia annuaL
Olofsson, L., Engström, A., Lundgren, A., & Brodelius, P. E. (2011) · BMC Plant Biology · doi:10.1186/1471-2229-11-45 · Cited by 144 · see citing works
A cDNA clone for β-caryophyllene synthase from Artemisia annua
Cai, Y., Jia, J.-W., Crock, J., Lin, Z.-X., Chen, X.-Y., & Croteau, R. (2002) · Phytochemistry · doi:10.1016/s0031-9422(02)00265-0 · Cited by 133 · see citing works
Production of artemisinin by genetically-modified microbes
Zeng, Q.-P., Qiu, F., & Yuan, L. (2007) · Biotechnology Letters · doi:10.1007/s10529-007-9596-y · Cited by 87 · see citing works
Effects of cobalt nanoparticles on artemisinin production and gene expression in Artemisia annua
GHASEMI, B., Hosseini, R., & NAYERI, F. D. (2015) · TURKISH JOURNAL OF BOTANY · doi:10.3906/bot-1410-9 · Cited by 86 · see citing works
Han, J., Wang, H., Ye, H., Liu, Y., Li, Z., Zhang, Y., Zhang, Y., Fang, Y., & Li, G. (2004) · Plant Science · doi:10.1016/j.plantsci.2004.07.020 · Cited by 67 · see citing works
Trichomes + roots + ROS = artemisinin: regulating artemisinin biosynthesis in Artemisia annua L.
Nguyen, K. T., Arsenault, P. R., & Weathers, P. J. (2011) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-011-9343-x · Cited by 66 · see citing works
Xie, D.-Y., Ma, D.-M., Judd, R., & Jones, A. L. (2016) · Phytochemistry Reviews · doi:10.1007/s11101-016-9480-2 · Cited by 63 · see citing works
Advances in the Plant Isoprenoid Biosynthesis Pathway and Its Metabolic Engineering
Liu, Y., Wang, H., Ye, H., & Li, G. (2005) · Journal of Integrative Plant Biology · doi:10.1111/j.1744-7909.2005.00111.x · Cited by 60 · see citing works
Suberu, J., Song, L., Slade, S. E., Sullivan, N., Barker, G. C., & Lapkin, A. A. (2013) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2013.06.025 · Cited by 48 · see citing works
Biotechnological approaches for artemisinin production in Artemisia
Kayani, W. K., Kiani, B. H., Dilshad, E., & Mirza, B. (2018) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-018-2432-9 · Cited by 37 · see citing works
Zeng, Q.-P., Chang, Z., Yin, L., Yang, R., Zeng, X.-M., Huang, Y., Feng, L.-L., & Yang, X. (2008) · Science in China Series C Life Sciences · doi:10.1007/s11427-008-0032-x · Cited by 34 · see citing works
Ma, D., Li, G., Alejos-González, F., Zhu, Y., Xue, Z. H., Wang, A., Zhang, H., Li, X., Ye, H., Wang, H., et al. (2017) · The Plant Journal · doi:10.1111/tpj.13583 · Cited by 32 · see citing works
Artemisinic acid: A promising molecule potentially suitable for the semi-synthesis of artemisinin
Kong, J., Yang, Y., Wang, W., Cheng, K.-D., & Zhu, P. (2013) · RSC Advances · doi:10.1039/c3ra40525g · Cited by 24 · see citing works
Zeng, Q.-P., Zeng, X.-M., Yin, L., Yang, R., Feng, L.-L., & Yang, X. (2008) · Plant Molecular Biology Reporter · doi:10.1007/s11105-008-0056-1 · Cited by 19 · see citing works
Chemistry, biosynthesis and biological activity of artemisinin and related natural peroxides
Rydén, A.-M., & Kayser, O. (2007) · Topics in Heterocyclic Chemistry · doi:10.1007/7081_2007_085 · Cited by 16 · see citing works
Sabir, F., Kumar, A., Tiwari, P., Pathak, N., Sangwan, R. S., Bhakuni, R. S., & Sangwan, N. S. (2010) · Zeitschrift für Naturforschung C · doi:10.1515/znc-2010-9-1013 · Cited by 13 · see citing works
Microbacterium trichotecenolyticum enzyme mediated transformation of arteannuin B to artemisinin
Tatineni, R., Doddapaneni, K. K., Dalavayi, S., Kulkarni, S. M., & Mangamoori, L. N. (2006) · Process Biochemistry · doi:10.1016/j.procbio.2006.05.025 · Cited by 11 · see citing works
Zhang, Y., Liu, B., Li, Z., Ye, H., Wang, H., Li, G., & Han, J. (2004) · Cited by 9 · see citing works
Effects of artificial polyploidy in transformed roots of Artemisia annua L.
Jesus, L. D. (2003) · Digital WPI · Cited by 5 · see citing works
Casteel, D. A. (2003) · Wiley eBooks · doi:10.1002/0471266949.bmc091 · Cited by 2 · see citing works
Biological Production of Artemisinin for Malaria Therapy
Zhao, Y., & Liu, C. (2006) · Wiley eBooks · doi:10.1002/9783527619771.ch23 · Cited by 1 · see citing works
Roots and hormones: synergistic control of artemisinin production in Artemisia annua L. shoots
Nguyen, K. V. (2011) · Digital WPI
青蒿素合成cDNA和新EST的克隆及其低温诱导表达的定量分析
曾庆平, 赵昌, 尹录录, 杨瑞仪, 曾晓梅, 黄瑛, 冯丽玲, & 杨雪芹 (2008) · Science in China Series C-Life Sciences (in Chinese) · doi:10.1360/zc2008-38-2-147
- Authors
- Dhingra, V., & Lakshmi Narasu, M.
- Affiliations
- Jawaharlal Nehru Technological University, Hyderabad (IN)
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2001-02
- Volume
- 281
- Issue
- 2
- Pages
- 558-561
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2058023698
- Cited by
- 27
- References
- 11
- Citations by year
- 2018: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2013: 2
- FWCI
- 0.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Keywords
- arteannuin B, artemisinin, Artemisia annua, enzyme purification, enzyme characterization, enzyme kinetics, pH optimum, optimal temperature
- MeSH terms
- Biotransformation, Electrophoresis, Polyacrylamide Gel, Enzymes, Chemical Fractionation, Hydrogen-Ion Concentration, Kinetics, Plant Proteins, Plants, Medicinal, Sesquiterpenes, Temperature, Artemisia, Artemisinins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review