Cited by 27Plant biochemistry and biosynthesisSesquiterpenes and Asteraceae StudiesPlant tissue culture and regeneration

Purification and Characterization of an enzyme involved in biochemical transformation of arteannuin B to artemisinin from Artemisia annua

Dhingra, Vikas, Lakshmi Narasu, M·2001·Biochemical and Biophysical Research Communications

Abstract

The protein involved in the conversion of arteannuin B to artemisinin has been purified from the leaves of Artemisia annua. The pure protein found to be homogenous on Native gel electrophoresis showed two major bands of 21 and 11 kDa on 12% SDS-PAGE. Molecular weight estimation of native protein indicated an apparent molecular mass of 66,000 Daltons. This protein is able to achieve 58% conversion. It has a K(m) of 0.5 mM for arteannuin B and a pH optima between 7.0-7.2. It is maximally active at 30 degrees C.

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Crossref · 10.1006/bbrc.2000.4197

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10 of 10 references verified0 citation errors · 0 not checkable

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  • A microbial system capable of converting arteannuin B (462) to artemisinin (495) has been described, and an enzyme with the appropriate activity has been purified.

    “A microbial system which is capable of converting arteannuin B (462) to artemisinin (495) has recently been described [309] and an enzyme with the appropriate activity has also been purified [310,311].”

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Authors
Dhingra, V., & Lakshmi Narasu, M.
Affiliations
Jawaharlal Nehru Technological University, Hyderabad (IN)
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2001-02
Volume
281
Issue
2
Pages
558-561
ISSN
0006-291X (ISSN-L)
OpenAlex
W2058023698
Cited by
27
References
11
Citations by year
2018: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2013: 2
FWCI
0.58
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
Keywords
arteannuin B, artemisinin, Artemisia annua, enzyme purification, enzyme characterization, enzyme kinetics, pH optimum, optimal temperature
MeSH terms
Biotransformation, Electrophoresis, Polyacrylamide Gel, Enzymes, Chemical Fractionation, Hydrogen-Ion Concentration, Kinetics, Plant Proteins, Plants, Medicinal, Sesquiterpenes, Temperature, Artemisia, Artemisinins

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