The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
En Passant Mutagenesis: A Two Step Markerless Red Recombination System
Abstract
Bacterial artificial chromosomes are used to maintain and modify large sequences of different origins in Escherichia coli. In addition to RecA-based shuttle mutagenesis, Red recombination is commonly used for sequence modification. Since foreign sequences, such as antibiotic resistance genes as well as frt- or loxP-sites are often unwanted in mutant BAC clones, we developed a Red-based technique that allows for the scarless generation of point mutations, deletions, and insertion of smaller and larger sequences. The method employs a sequence duplication that is inserted into the target sequence in the first recombination step and the excision of the selection marker by in vivo I-SceI cleavage and the second Red recombination. To allow for convenient and highly efficient mutagenesis without the use of additional plasmids, the E. coli strain GS1783 with a chromosomal encoded inducible Red- and I-SceI-expression was created.
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Self-citationUse of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli
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Hammer, Q., Rückert, T., Borst, E. M., Dunst, J., Haubner, A., Durek, P., Heinrich, F., Gasparoni, G., Babic, M., Tomic, A., et al. (2018) · Nature Immunology · doi:10.1038/s41590-018-0082-6 · Cited by 423
Gassen, N. C., Niemeyer, D., Muth, D., Corman, V. M., Martinelli, S., Gassen, A., Hafner, K., Papies, J., Mösbauer, K., Zellner, A., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-13659-4 · Cited by 356
Ogando, N. S., Zevenhoven-Dobbe, J. C., van der Meer, Y., Bredenbeek, P. J., Posthuma, C. C., & Snijder, E. J. (2020) · Journal of Virology · doi:10.1128/jvi.01246-20 · Cited by 282
Multi-layered stochasticity and paracrine signal propagation shape the type-I interferon response
Rand, U., Rinas, M., Schwerk, J., Nöhren, G., Linnes, M., Kröger, A., Flossdorf, M., Kály‐Kullai, K., Hauser, H., Höfer, T., et al. (2012) · Molecular Systems Biology · doi:10.1038/msb.2012.17 · Cited by 147
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Metadata
- Authors
- Tischer, B. K., Smith, G. A., & Osterrieder, N.
- Venue
- Methods in Molecular Biology
- Publisher
- Humana Press
- Published
- 2010
- Volume
- 634
- Pages
- 421-430
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W1851333263
- Cited by
- 599
- References
- 12
- FWCI
- 27.56
- Type
- book chapter
- Language
- en
- Open access
- closed
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- Tischer, B. K., Smith, G. A., & Osterrieder, N. (2010). En Passant Mutagenesis: A Two Step Markerless Red Recombination System. Methods in Molecular Biology. https://doi.org/10.1007/978-1-60761-652-8_30
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