Effects of sleep-deprivation on the activity of selected metabolic enzymes in skeletal-muscle
Abstract
The effect of 120-h sleep deprivation on the activity of selected enzymes of energy metabolism in skeletal muscle was studied in seven healthy volunteers. The results showed a significant decrease in the activity of malate dehydrogenase, citrate synthase, glycerol-3-phosphate dehydrogenase and lactate dehydrogenase. Triosephosphate dehydrogenase, hexokinase, and hydroxyacyl-CoA-dehydrogenase activities showed an insignificant decrease. The findings are indicative of (1) decreased aerobic oxidation capacity; (2) reduced function of reducing-equivalent carriers from cytosol across the mitochondrial membrane; (3) relative accentuation of the non-aerobic glycolytic pathway; (4) a prediabetic type of muscle metabolism.
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120 h of sleep deprivation was associated with a 24% reduction in citrate synthase activity in human skeletal muscle.
“In support of this, 120 h of sleep deprivation was associated with a 24% reduction in citrate synthase activity in human skeletal muscle [18].”
From Exercise mitigates sleep-loss-induced changes in glucose tolerance, mitochondrial function, sarcoplasmic protein synthesis, and diurnal rhythms (2021) · cites this paper as [18]
Increased fasting glucose was reported after 120 hours of total sleep deprivation but not after 72-126 hours or one night of total sleep deprivation.
“Increased fasting glucose was reported after 120 h of TSD [200] but not after 72–126 h [86] or one single night of TSD [5, 143, 195, 204]”
From Sleep and metabolic function (2012) · cites this paper as [200]
Fasting blood glucose levels were increased at the end of 120 hours of total sleep deprivation.
“fasting blood glucose levels were increased at the end of the sleep deprivation period [13].”
From Role of sleep duration in the regulation of glucose metabolism and appetite (2010) · cites this paper as [13]
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- Authors
- Vondra, K., Brodan, V., Bass, A., Kuhn, E., Teisinger, J., AndĚl, M., & Veselková, A.
- Affiliations
- Institute of Clinical and Experimental Medicine (CZ)
- Czech Academy of Sciences (CZ)
- ORCID
- Veselková, A.
- Venue
- European Journal of Applied Physiology and Occupational Physiology
- Published
- 1981-08
- Volume
- 47
- Issue
- 1
- Pages
- 41-46
- ISSN
- 0020-9376 (ISSN-L)
- 0301-5548
- 1439-6327
- OpenAlex
- W2023765776
- Cited by
- 53
- References
- 7
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 4 · 2017: 1 · 2016: 1 · 2015: 4 · 2014: 1 · 2013: 1 · 2012: 3
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- European Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Keywords
- sleep deprivation, skeletal muscle, energy metabolism enzymes, malate dehydrogenase, citrate synthase, glycerol-3-phosphate dehydrogenase, lactate dehydrogenase, triosephosphate dehydrogenase, hexokinase, hydroxyacyl-CoA dehydrogenase, glycolysis, prediabetes, mitochondrial membrane transport
- MeSH terms
- Adult, Citrate (si)-Synthase, Energy Metabolism, Glyceraldehyde-3-Phosphate Dehydrogenases, Glycerolphosphate Dehydrogenase, Hexokinase, Humans, L-Lactate Dehydrogenase, Malate Dehydrogenase, Male, Muscles, Sleep Deprivation, 3-Hydroxyacyl CoA Dehydrogenases
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