Influence of C-phycocyanin on hepatocellular parameters related to liver oxidative stress and Kupffer cell functioning
Open accessAbstract
Objectives Kupffer cells, liver macrophages involved in immunomodulation, phagocytosis, and biochemical attack, can induce cytotoxicity and inflammation when their activity is exacerbated. The aim of this study was to evaluate the effects of C-phycocyanin on Kupffer cell functioning considering its antioxidant and anti-inflammatory properties.Materials and methods Actions of C-phycocyanin on colloidal carbon phagocytosis, carbon-induced respiratory burst activity, and sinusoidal lactate dehydrogenase (LDH) release were studied in isolated perfused mouse liver. The influence of C-phycocyanin on tumor necrosis factor-a (TNF-alpha) and nitrite levels in serum and liver nitric oxide synthase (NOS) activity was assessed in rats subjected to thyroid hormone (T3) administration, a condition known to underlie hepatic oxidative stress comprising an increased Kupffer cell activity.Results C-phycocyanin elicited a concentration-dependent inhibition of carbon phagocytosis and carbon-induced O2 uptake (IC50 = 0.2 mg/ml) by perfused livers, with a 52% diminution in the carbon-induced sinusoidal release of LDH being found at a concentration of 0.25 mg/ml. Thyroid calorigenesis induced an 82-fold increase in serum TNF-alpha levels, an effect that was suppressed by pretreatment with C-phycocyanin, the antioxidant alpha-tocopherol, and by the Kupffer cell inactivator gadolinium chloride. C-phycocyanin also suppressed the T3-induced increases in serum nitrite levels (234%) and in the activity of hepatic NOS (75%).Conclusions C-phycocyanin significantly decreases Kupffer cell phagocytosis and the associated respiratory burst activity, effects that may contribute to the abolition of oxidative stress-induced TNF-alpha response and NO production by hyperthyroid state.
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C-phycocyanin strongly inhibits inflammation in macrophages and animal models.
“In particular, C-PC, a natural blue pigment (~14% of SP dry weight) [40, 43, 44], has shown to have a strong inhibitory property against inflammation in macrophages [45, 46] and in animal models [47, 48].”
From Edible blue-green algae reduce the production of pro-inflammatory cytokines by inhibiting NF-κB pathway in macrophages and splenocytes (2013) · cites this paper as [46]
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- Authors
- Remirez, D., Fernández, V., Tapia, G., González, R., & Videla, L. A.
- Affiliations
- University of Chile (CL)
- Centro Nacional de Investigaciones Científicas (CU)
- Venue
- Inflammation Research
- Published
- 2002-07
- Volume
- 51
- Issue
- 7
- Pages
- 351-356
- ISSN
- 1023-3830 (ISSN-L)
- OpenAlex
- W2033555258
- Cited by
- 63
- References
- 30
- Citations by year
- 2025: 3 · 2024: 4 · 2023: 1 · 2022: 5 · 2021: 2 · 2020: 3 · 2019: 5 · 2018: 1 · 2017: 5 · 2016: 5 · 2015: 5 · 2014: 2 · 2013: 4 · 2012: 1
- FWCI
- 0.19
- Type
- journal article
- Language
- en
- Open access
- green
- americanae.aecid.es
- Licence
- other-oa
- www.springer.com/tdm (tdm)
- Locations
- Inflammation Research (publishedVersion)
- PubMed (publishedVersion)
- Americanae (AECID Library) (submittedVersion, other-oa)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
- Universidad de Chile (submittedVersion, cc-by-nc-nd)
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
- Keywords
- C-phycocyanin, Kupffer cells, phagocytosis, respiratory burst, lactate dehydrogenase release, TNF-α, nitric oxide, nitric oxide synthase, thyroid hormones, hyperthyroidism, antioxidant activity, anti-inflammatory activity, alpha-tocopherol, gadolinium chloride, liver inflammation
- MeSH terms
- Animals, Carbon, Enzyme-Linked Immunosorbent Assay, Kupffer Cells, L-Lactate Dehydrogenase, Liver, Nitrites, Oxygen Consumption, Phycocyanin, Triiodothyronine, Tumor Necrosis Factor-alpha, Respiratory Burst, Oxidative Stress, Nitric Oxide Synthase, Hepatocytes, Mice, Rats, In Vitro Techniques
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