Cited by 477Antioxidant Activity and Oxidative StressPhytochemicals and Antioxidant ActivitiesSeaweed-derived Bioactive Compounds

C-Phycocyanin: A potent peroxyl radical scavenger in Vivo and in Vitro

Bhat, Vadiraja B., Madyastha, K.M.·2000·Biochemical and Biophysical Research Communications

Abstract

C-Phycocyanin (from Spirulina platensis) effectively inhibited CCl(4)-induced lipid peroxidation in rat liver in vivo. Both native and reduced phycocyanin significantly inhibited peroxyl radical-induced lipid peroxidation in rat liver microsomes and the inhibition was concentration dependent with an IC(50) of 11.35 and 12.7 microM, respectively. The radical scavenging property of phycocyanin was established by studying its reactivity with peroxyl and hydroxyl radicals and also by competition kinetics of crocin bleaching. These studies have demonstrated that phycocyanin is a potent peroxyl radical scavenger with an IC(50) of 5.0 microM and the rate constant ratios obtained for phycocyanin and uric acid (a known peroxyl radical scavenger) were 1.54 and 3.5, respectively. These studies clearly suggest that the covalently linked chromophore, phycocyanobilin, is involved in the antioxidant and radical scavenging activity of phycocyanin.

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Crossref · 10.1006/bbrc.2000.3270

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Authors
Bhat, V. B., & Madyastha, K. M.
Affiliations
Indian Institute of Science Bangalore (IN)
Corresponding author
Madyastha, K.M.
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2000-08
Volume
275
Issue
1
Pages
20-25
ISSN
0006-291X (ISSN-L)
OpenAlex
W2085946002
Cited by
477
References
22
Citations by year
2026: 14 · 2025: 18 · 2024: 15 · 2023: 16 · 2022: 29 · 2021: 13 · 2020: 15 · 2019: 21 · 2018: 24 · 2017: 17 · 2016: 16 · 2015: 31 · 2014: 23 · 2013: 24 · 2012: 23
FWCI
3.31
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
  • NOT FOUND REPOSITORY (Indian Institute of Science Bangalore) (acceptedVersion)
Topics
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
Keywords
C-phycocyanin, phycocyanin, peroxyl radical scavenging, lipid peroxidation, antioxidant activity, phycocyanobilin, Spirulina platensis, liver microsomes, hydroxyl radicals, uric acid
MeSH terms
Animals, Antioxidants, Bacterial Proteins, Borohydrides, Carbon Tetrachloride, Carotenoids, Free Radicals, Kinetics, Microsomes, Liver, Oxidation-Reduction, Peroxides, Phycocyanin, Pyrroles, Spectrophotometry, Mass Spectrometry, Uric Acid, Lipid Peroxidation, Free Radical Scavengers, Hydroxyl Radical, Inhibitory Concentration 50, Tetrapyrroles, Rats, Phycobilins, Spirulina

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