C-Phycocyanin: A potent peroxyl radical scavenger in Vivo and in Vitro
Abstract
C-Phycocyanin (from Spirulina platensis) effectively inhibited CCl(4)-induced lipid peroxidation in rat liver in vivo. Both native and reduced phycocyanin significantly inhibited peroxyl radical-induced lipid peroxidation in rat liver microsomes and the inhibition was concentration dependent with an IC(50) of 11.35 and 12.7 microM, respectively. The radical scavenging property of phycocyanin was established by studying its reactivity with peroxyl and hydroxyl radicals and also by competition kinetics of crocin bleaching. These studies have demonstrated that phycocyanin is a potent peroxyl radical scavenger with an IC(50) of 5.0 microM and the rate constant ratios obtained for phycocyanin and uric acid (a known peroxyl radical scavenger) were 1.54 and 3.5, respectively. These studies clearly suggest that the covalently linked chromophore, phycocyanobilin, is involved in the antioxidant and radical scavenging activity of phycocyanin.
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Algae-derived C-phycocyanin successfully reduced lipid peroxidation in liver microsomes from CCl4-intoxicated rats.
“Algae-derived C-phycocyanin successfully reduced lipid peroxidation in liver microsomes from CCl4-intoxicated rats [116].”
From Application of Arthrospira platensis for Medicinal Purposes and the Food Industry: A Review of the Literature (2023) · cites this paper as [116]
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- Authors
- Bhat, V. B., & Madyastha, K. M.
- Affiliations
- Indian Institute of Science Bangalore (IN)
- Corresponding author
- Madyastha, K.M.
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2000-08
- Volume
- 275
- Issue
- 1
- Pages
- 20-25
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2085946002
- Cited by
- 477
- References
- 22
- Citations by year
- 2026: 14 · 2025: 18 · 2024: 15 · 2023: 16 · 2022: 29 · 2021: 13 · 2020: 15 · 2019: 21 · 2018: 24 · 2017: 17 · 2016: 16 · 2015: 31 · 2014: 23 · 2013: 24 · 2012: 23
- FWCI
- 3.31
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- NOT FOUND REPOSITORY (Indian Institute of Science Bangalore) (acceptedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- C-phycocyanin, phycocyanin, peroxyl radical scavenging, lipid peroxidation, antioxidant activity, phycocyanobilin, Spirulina platensis, liver microsomes, hydroxyl radicals, uric acid
- MeSH terms
- Animals, Antioxidants, Bacterial Proteins, Borohydrides, Carbon Tetrachloride, Carotenoids, Free Radicals, Kinetics, Microsomes, Liver, Oxidation-Reduction, Peroxides, Phycocyanin, Pyrroles, Spectrophotometry, Mass Spectrometry, Uric Acid, Lipid Peroxidation, Free Radical Scavengers, Hydroxyl Radical, Inhibitory Concentration 50, Tetrapyrroles, Rats, Phycobilins, Spirulina
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